1R8G


Conserved Protein Domain Family
GCS2

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pfam04107: GCS2 
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Glutamate-cysteine ligase family 2(GCS2)
Also known as gamma-glutamylcysteine synthetase and gamma-ECS (EC:6.3.2.2). This enzyme catalyzes the first and rate limiting step in de novo glutathione biosynthesis. Members of this family are found in archaea, bacteria and plants. May and Leaver discuss the possible evolutionary origins of glutamate-cysteine ligase enzymes in different organisms and suggest that it evolved independently in different eukaryotes, from an ancestral bacterial enzyme. They also state that Arabidopsis thaliana gamma-glutamylcysteine synthetase is structurally unrelated to mammalian, yeast and Escherichia coli homologs. In plants, there are separate cytosolic and chloroplast forms of the enzyme.
Statistics
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PSSM-Id: 397982
Aligned: 12 rows
Threshold Bit Score: 231.516
Threshold Setting Gi: 62899815
Created: 11-May-2020
Updated: 7-Aug-2020
Structure
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Program:
Drawing:
Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
1R8G_A    14 LGIELEXQVVNPPGYDLS--QdSSXLIDAVKNKIT---AGEVKHDITESXLELATD-VCRDINQAAGQFSAXQKVVLQAA 87  Escherichia coli
Q9I1T4    12 FGIEEEFFLLDASDLDIV--R-SAPAGFVAACRDTl--GEHFAEEMFECQVEVASP-VFSTLAEAARFHGQARQRLAHLA 85  Pseudomonas aerugin...
P74515     5 KGLEVEIYTGKKTGEIVG-------LSDRIVRDLS-----GFVREPDSRNVEYTTP-PLNSYDRLLCGILQPRRQLRHYL 71  Synechocystis sp. P...
Q9KY07     4 VGVEEELLLVDPATGEPR--AlSAAVLARAFLDDSe--QDVFEKELHEQMLEFATH-PQADMERLHAEIVRCREEAGRHA 78  Streptomyces coelic...
O27977    12 DENFNPVPISDEILKKIG-----GRTVNEAKLGRV-----VIGKELQKHVIELKPArPFESLSEFEETMQEGVEELLSVM 81  Archaeoglobus fulgi...
Q9A2Z2    50 VPYEGDKGVHALLTGLQRf-GwTPVMEGPTIIGLEr--NGANVSLEPGGQFELSGA-PLSTMHEICEETGQHLDEVKTVA 125 Caulobacter vibrioides
AVZ42721  53 pSYEEKGGICDLLQGFTRf-GwQPIYENDKIIGLSg--DDGAISLEPAGQFELSGA-PRSTIHESYDEICRHIQQTQEVG 128 Zymomonas mobilis s...
Q9RD01    37 LGVEVEWLVHELRAPRLPv---SPERLQAVYTALRavpLRSALTVEPGGQLELSSL-PAASLTECVRSVSADLEAVRAVL 112 Streptomyces coelic...
P9WPK7    46 VGLEMEAHCFDPADPFRRp-SwEEITE--VLEWLSplpGGSVVSVEPGGAVELSGP-PADGVLAAIGAMTRDQAVLRSAL 121 Mycobacterium tuber...
P9WPK9    20 LGVEWEFALVDSQTRDLS----NEATAVIAEIGEN----PRVHKELLRNTVEIVSG-ICECTAEAMQDLRDTLGPARQIV 90  Mycobacterium tuber...
Q9HPZ9    15 LGVEEEFFVVDEHGVPTAg---SDELVYEGEPPEPi--AGRLDHELFKFVVETQTP-TLNGVAEAPAAIREVRAALVAYA 88  Halobacterium salin...
Q89V83    50 VPYEGARGIGALLEGMKLllGwEPIMEKGNIIGLYdvtGGGAISLEPGGQFELSGA-PVENVHQTQSELMAHLAQVREIA 128 Bradyrhizobium japo...
1R8G_A    88 TDHHLEICGGGTHPFQKWQRQEVCDNERYQRTLENFGY----L-IQQATVFGQHVHVGCASGDDAI------YLLHGLSR 156 Escherichia coli
Q9I1T4    86 MDFGLRSLCVGTHPFADWRRARSNPAAHFARLFEDQGR----V-ARRSLVCGLHVHVEIPPSHDRM------AVLQRVLP 154 Pseudomonas aerugin...
P74515    72 SQLGEDYTLIPGSCLALGGTDTFYRSDPQNPYHTYIEQ----TySTQVVTASIHINIGIPDVEELI------RACRLVRM 141 Synechocystis sp. P...
Q9KY07    79 GGIGCAVAALATSP------LPVTPSIGVNRRYEWMAEqy-gVvVHEQLVLGCHVHVSVDS-DEEGv-----AVIDRVRP 145 Streptomyces coelic...
O27977    82 D--GYKLLGLGMHP-LLRLEDAKVWNHRDRRIYQAYDRl--fNiKQHGWLNIQSYQLNIPFSSEKEa----vELHNKIRV 152 Archaeoglobus fulgi...
Q9A2Z2   126 DELGLGFTGLGFSPKWTRGQVPIMPKGRYVIMRNYMPKv--gNlGLDMMLRTCTVQANLDFSSEADm----vAKFRMSLA 199 Caulobacter vibrioides
AVZ42721 129 DELGLGFLGLGLWPDKKRSDLPLMPKGRYKIMTEYMPKv--gKlGLDMMLRTCTIQSNIDYGSEADm----vKKFRVSLA 202 Zymomonas mobilis s...
Q9RD01   113 REDGLALVGLGHDP-WHPPRRYLRQP-RYDAMESCLDRt--gPgGRFMMCASASVQVCVDAGHEEPgplghvRRWWLAHH 188 Streptomyces coelic...
P9WPK7   122 ANAGLGLVFLGADP--LRSPVRVNPGARYRAMEQFFAAshsgVpGAAMMTSTAAIQVNLDAGPQEGw----aERVRLAHA 195 Mycobacterium tuber...
P9WPK9    91 RDRGMELFCAGTHPFARWSAQKLTDAPRYAELIKRTQW----W-GRQMLIWGVHVHVGIRSAHKVM------PIMTSLLN 159 Mycobacterium tuber...
Q9HPZ9    89 SEHGLRIAGAGLHPGARWREHEHAEKPRYRSQLDRIQY----P-QHRNTTAGLHIHVGVDD-PDKAv-----WVSNRMRW 157 Halobacterium salin...
Q89V83   129 APLGIGFLGLGMTPSWSRADIPVMPKGRYKIMTNYMPKv--gQyGLDMMYRTCTVQTNLDFSSEADm----vKKLRVSLA 202 Bradyrhizobium japo...
1R8G_A   157 FVPHFIALSAASPYXQGTDTRFASSRPNIFSAFPDn--GPXPWVSNWQQ-FEALFRCLSYTTXIDSIK------------ 221 Escherichia coli
Q9I1T4   155 WLPLLLALSASSPFRGGRRSGLASYRRALCGEWPR---MNIPPALPDEDaYRRHLALLRETGCIREDG------------ 219 Pseudomonas aerugin...
P74515   142 EAPLYLALSASSPFLNGQVTGYHSSRWQMFPKTPQ----EVPLFTSHGh-FVQWTEEQLTLGTMQNVR------------ 204 Synechocystis sp. P...
Q9KY07   146 WLPVLAALSANSPFWQGRDSSYSSYRSRVWQRWPSa--GPTELFGSA----ERYHRRVADMLATGTVLddgm-------- 211 Streptomyces coelic...
O27977   153 LLPYIAAVASASPICEGK-SYYVDTR-LYFYRINQ---KEVPEICNDVIp-ERISSLKEYRSILN--------------- 211 Archaeoglobus fulgi...
Q9A2Z2   200 LQPIATALFANSPFTEGKPNGFLSARANVWTDTDPnrtGLLDFVFEDGFdFERYARYALDVPMYFVKRgdkyid---vag 276 Caulobacter vibrioides
AVZ42721 203 LQPLATALFANSPFLEGHPNGFSSYRSHIWTDTDPhrtGILPFVFDDDFgYERYIDYMLSVPMYFVYRdgryid---asg 279 Zymomonas mobilis s...
Q9RD01   189 LGAVLLAAFANSPLSGGRPTGWRSTRQLRWTQIGAgraGGPPLDSDP---RGTWARHVLDAPVMCVRRdggpwdv--peg 263 Streptomyces coelic...
P9WPK7   196 LGPTMIAIAANSPMLGGRFSGWQSTRQRVWGQMDSarcGPILGASGDHP-GIDWAKYALKAPVMMVRSpdtqdtravtdy 274 Mycobacterium tuber...
P9WPK9   160 YYPHLLALSASSPWWGGEDTGYASNRAMMFQQLPT---AGLPFHFQRWAeFEGFVYDQKKTGIIDHMD------------ 224 Mycobacterium tuber...
Q9HPZ9   158 HMPVLLALSANSPYWNGFDTGLASARAKIFEGLPN---TGLPTAFESYAaFQAFERRMVEHGGIEDRGe----------- 223 Halobacterium salin...
Q89V83   203 LQPVATALFANSPFTEGKPNGFLSFRSEIWRDTDNaraGMMPWAFEDGMgFERYVDYALDVPMYFVKRgedyid---vsg 279 Bradyrhizobium japo...
1R8G_A   222 ---DLHWDIRPSP-HFG----TVEVRVXDTP-----LTLSHAVNXAGLIQATAHWLLTERPFKHQEKDYLL--YKFNRFQ 286 Escherichia coli
Q9I1T4   220 ---QVWWMIRPSS-HVP----TLELRICDAC-----PRLADALSLAGLFRALVGEALGADPRALPVARDAC--LEENYWQ 284 Pseudomonas aerugin...
P74515   205 ---HLWSAVRPNGdNRPhnlnRLELRICDLVtnplsLLAIVALLEARLQQMLADPTLDPLQQSQLTPSELLalADQNEIS 281 Synechocystis sp. P...
Q9KY07   212 vyfDVRLSQRYPT-VEFr---VADVCLDAST-----AVVLAALARALVDTAAREWRAGAEPAEHSVSLLRL--A---AWR 277 Streptomyces coelic...
O27977   212 ---GIYEELRRKGaYVL----CREWVNSRGVi----VRFSRKCLEIKVMDEQECIKSDVALTAFIRAILRAe-LEELPVD 279 Archaeoglobus fulgi...
Q9A2Z2   277 rsfRDFIEGKLPE-LPGeiatMKDFADHTTTaf--pEVRLKTYLEMRGADAGPWSRLCALPALWTGVFYDDa-ALAAAWD 352 Caulobacter vibrioides
AVZ42721 280 qdfRAFLRGELPA-LPNekpiLSDWVDHLSTaf--pEVRLKSYLEMRGADGASAMMSPALSAFWISILYDSe-LLDTASD 355 Zymomonas mobilis s...
Q9RD01   264 ltlREWSRELVP--RPPt---REDLDYHLTTlf--pPVRPRGHLELRMIDAQPGDDGWIVPLAVTAALFDDpeATETAYR 336 Streptomyces coelic...
P9WPK7   275 vpfTDWVDGRVL--LDGrratVADLVYHLTTlf--pPVRPRQWLEIRYLDSVP-DEVWPAVVFTLVTLLDDpvAADLAVD 349 Mycobacterium tuber...
P9WPK9   225 ---EIRWDIRPSP-HLG----TLEVRICDGVsn--lRELGALVALTHCLIVDLDRRLDAGETLPTMPPWHV---QENKWR 291 Mycobacterium tuber...
Q9HPZ9   224 ----LWYDVRPHS-GHG----TVEVRAPDAQad--pAVVDAFVEYAHALVTEYAQRYDDHPDPFSVTGLRRelLDANKWR 292 Halobacterium salin...
Q89V83   280 ssfRAFFDGRNNN-LPGerptLSDWANHLSTif--pEVRLKRYLEMRGSDGGPWGRLPALPAFWVGLLYDDv-SLDAAWD 355 Bradyrhizobium japo...
1R8G_A   287 ACRYGL 292 Escherichia coli
Q9I1T4   285 ALRYGC 290 Pseudomonas aeruginosa PAO1
P74515   282 AAVSSL 287 Synechocystis sp. PCC 6803
Q9KY07   278 AARSGL 283 Streptomyces coelicolor A3(2)
O27977   280 VLIEKL 285 Archaeoglobus fulgidus DSM 4304
Q9A2Z2   353 LCKDWT 358 Caulobacter vibrioides
AVZ42721 356 IIKSWS 361 Zymomonas mobilis subsp. mobilis ZM4 = ATCC 31821
Q9RD01   337 AVKPLA 342 Streptomyces coelicolor
P9WPK7   350 AVEPVa 355 Mycobacterium tuberculosis H37Rv
P9WPK9   292 AARYGL 297 Mycobacterium tuberculosis H37Rv
Q9HPZ9   293 AMRDGH 298 Halobacterium salinarum NRC-1
Q89V83   356 MVKHWS 361 Bradyrhizobium japonicum
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