6MUJ,5HHA


Conserved Protein Domain Family
FGE-sulfatase

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pfam03781: FGE-sulfatase 
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Sulfatase-modifying factor enzyme 1
This domain is found in eukaryotic proteins required for post-translational sulfatase modification (SUMF1). These proteins are associated with the rare disorder multiple sulfatase deficiency (MSD). The protein product of the SUMF1 gene is FGE, formylglycine (FGly),-generating enzyme, which is a sulfatase. Sulfatases are enzymes essential for degradation and remodelling of sulfate esters, and formylglycine (FGly), the key catalytic in the active site, is unique to sulfatases. FGE is localized to the endoplasmic reticulum (ER) and interacts with and modifies the unfolded form of newly synthesized sulfatases. FGE is a single-domain monomer with a surprising paucity of secondary structure that adopts a unique fold which is stabilized by two Ca2+ ions. The effect of all mutations found in MSD patients is explained by the FGE structure, providing a molecular basis for MSD. A redox-active disulfide bond is present in the active site of FGE. An oxidized cysteine residue, possibly cysteine sulfenic acid, has been detected that may allow formulation of a structure-based mechanism for FGly formation from cysteine residues in all sulfatases. In Mycobacteria and Treponema denticola this enzyme functions as an iron(II)-dependent oxidoreductase.
Statistics
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PSSM-Id: 397722
Aligned: 22 rows
Threshold Bit Score: 188.09
Threshold Setting Gi: 75341128
Created: 11-May-2020
Updated: 7-Aug-2020
Structure
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Program:
Drawing:
Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
6MUJ_A  27 STRGQVRLPGGEFAMGDaf--------------geGYPADGETPVHTVRL-RPFHIDETAVTNARFAAFVKA---TGHVT 88  Streptomyces coelicol...
Q9KYH7   4 EAVDWVEIPAGVVRRGTpaddiplvaeryadtgvpVEWYLKEAPSTEIRL-PAFRLARTPVTVGQWSVFAAA---TGRP- 78  Streptomyces coelicolor
Q9KKM6 381 SAPEVITILPGQYLLG-------------------------ENAAKQYNLdHAFALSSTPVTVSQFENFVTQ---TKYKT 432 Vibrio cholerae
5HHA_A  38 DCPEMVVLPAGSFTMGTpd--------------deVGRQPDEGPLHDVTFaKPFAISRYQVTAGELDAYLKAtgvK---- 99  Pseudomonas aeruginos...
Q9PFC6 346 RGPQMLVVPHGSFQMGAtd--------------teIGASAAERPQHDVRFeRGFAMSITEVTVAQFRRFVEQ---TGYRP 408 Xylella fastidiosa
O34722 201 EEDGMILIPGGFFEFGLnene-----------ipdKTFNWKDAVPRQKVWlPPFFIDKYPVTNKDYDIFTEFieeNGHI- 268 Bacillus subtilis sub...
P72841 165 LEATMVPIPAGGFYQGSe----------------qIWAQDNERPVHWVEV-EQFWLDKYPVTQAQYQQFIDA---GGYQQ 224 Synechocystis sp. PCC...
O69671 163 MAGTSVLVAGGPFVLGVdaa-------------dePCSLDNERPAHVVDV-PAFRIGRVPVTNGEWQDFIDD---GGYTQ 225 Mycobacterium tubercu...
Q9RD00 179 TGPAEVLVPGGPFAMGTst---------------ePWALDNERPAHRREV-APYFIDTVPVTNGAYRAFIDD---GGYDD 239 Streptomyces coelicolor
P0DPS7 376 LLTEMIFIEGGEFSRGS------------------GDGQRDELPVHNITL-PGFFLDIHPVTNEQFVRFLEc---VG--S 431 Chlamydia trachomatis...
6MUJ_A  89 DaerfgssavfhlvvaapdadvlgsaAGAPWWINVRGAHWRRPEGARSDITGRPNHPVVHVSWNDATAYARWAGK----- 163 Streptomyces coelicol...
Q9KYH7  79 -----------------------------------------VPTA-------PHDHPVTGVSWEAASAYCRWLGElsgdp 110 Streptomyces coelicolor
Q9KKM6 433 Dae---------------------lkNLCISVNESEIAPVSDSDWRNPGFKQSKDSPVVCVSQNDAKAYARWLSKetg-f 490 Vibrio cholerae
5HHA_A 100 -------------------------------LADGDTRPGRECIAGKPRYQQGPRQPAVCVDYNDVKNYAAWLSKktg-k 147 Pseudomonas aeruginos...
Q9PFC6 409 Ratrr-----------------ghslVYDERSGNFVRRSGVDWQSGYDGRVAAPNDPVMHVSVRDAEAYAAWMSRqta-r 470 Xylella fastidiosa
O34722 269 ------------------------------FCHPNEPQNKQHRRNTYWDDRYLDNHPVTGIDFYDAFAYARYKGK----- 313 Bacillus subtilis sub...
P72841 225 Sk-----------------------yWTEAGWQWRSMANVTAPLYWHP-ESNTQNHPVYGVSAYEAEAYANFVGK----- 275 Synechocystis sp. PCC...
O69671 226 Srwwsergw---------qhrqraglTAPQFWRSGGRTRTRFGH----VEDIPADEPVQHVSYFEAEAYAAWAGA----- 287 Mycobacterium tubercu...
Q9RD00 240 Prwwtpegw---------dhirrhavTAPLFWRRDGGQWLRRRFG--VTEAVPADEPVLHVCWYEADAYARWAGR----- 303 Streptomyces coelicolor
P0DPS7 432 Eq-----------------------dEHYNELIRLKDSRIQRRSGRLIIEPGYAKHPVVGVTWYGASSYACWIGK----- 483 Chlamydia trachomatis...
6MUJ_A 164 --RLPTEAEWEYAARGGL---AGRRYAWGDELTPGGrw-----rcNIWQGrf--pHVNTAEDG---HLS-TAPVKSYRP- 226 Streptomyces coelicol...
Q9KYH7 111 giRLPTEDEWERAARGd----DGREFPWGDEYR------------------------TGLANLvdlGVGtTTPVGSFPDg 162 Streptomyces coelicolor
Q9KKM6 491 tyRLPTEEEWEIAARAGS---K-TDYWWGNKFGa--------------------gKANTGWAGtswSNKsTSPVKAFAP- 545 Vibrio cholerae
5HHA_A 148 ryRMLSEAEREYGARAGS---AGPFPFPFDEGKEYS---------Ia--------KHANTYGAsdgYNF-TSPVGSFPP- 205 Pseudomonas aeruginos...
Q9PFC6 471 yyRLPSEAEFEYALRAGR----QGYYPWGNAAVPVTgny----tgGNDVSps-grRWANAFIGygdRYWaVAPVASFRA- 540 Xylella fastidiosa
O34722 314 --ELPTEFQWEKAARGe----KGNVWPWGDKFDPAKvqyagslynEPITSlkswrENLLKAHAdkeLNHlTSDIFEQNGe 387 Bacillus subtilis sub...
P72841 276 --RLPTEQEWERAARWSGq-hNSSTYPWEETLP------------------------TKEQCNfnnHRGrTTPVHYYDQg 328 Synechocystis sp. PCC...
O69671 288 --RLPTEVEWEKACAWDPatgSRRRYPWGTE------------------------EPTDTYANlggQTLrPAPVGAYPAg 341 Mycobacterium tubercu...
Q9RD00 304 --RLPTEAEWEKAARYDPagdRAMRYPWGDA------------------------DPGPEHANlgqRHLrPAPAGSYPAg 357 Streptomyces coelicolor
P0DPS7 484 --RLPSEAEWEVAASGGK---LGLRYPTGEEID---------------------------KSKanfFSSdTTPVMSYPS- 530 Chlamydia trachomatis...
6MUJ_A 227 -NGHGLWNTAGNVWEWCSDWFSptyyaes--ptvdphgPGTGAARVLRGGSYLCHDsYCNRYRVAAR-SSnTPDSSSGNL 302 Streptomyces coelicol...
Q9KYH7 163 aGPFGVLDMAGNVDEWTSTLYApypgapa---gvplveDWAFDPHITRGGAFRHD---RDLARCARRhSAyEEDLAAIGV 236 Streptomyces coelicolor
Q9KKM6 546 -NALGFYDMVGNVWEWTGD-----------------------SRGLAKGGAWSFS---PEMAKAYSElFVg-PTTAANYV 597 Vibrio cholerae
5HHA_A 206 -NAFGVYDMHGNVYEWVADCWHdhyngap--sdgsawmEEKCELVQIRGNDWGE---PPIFSRSGNR-NNaAPSDRGDWI 278 Pseudomonas aeruginos...
Q9PFC6 541 -NAWGLHDMGGNLSEWVADCWHssyrrap--adgvawyNPGCRQRLVRGGSWANS---PQQTRAAWR-GSqDSEMTSARI 613 Xylella fastidiosa
O34722 388 -SPYGVCGMIGGTWEWTRSELKtkrafhp--ifenvpfNSVNSFAVLKGGSFFSH---PGLMFPSFRaKD-IPFCRHNEM 460 Bacillus subtilis sub...
P72841 329 rNQGGCYDLLGNVWEWTASTFEpydnfqpypyrgysqaYFDGEHRVMRGGSWATR---SWGLRASFR-NWyHPWTRQVLV 404 Synechocystis sp. PCC...
O69671 342 aSACGAEQMLGDVWEWTTSPLRpwpgfvpmvyerysqpFFGGDYRVLRGGSWAVE---PAILRPSFR-NWdHPYRRQIFA 417 Mycobacterium tubercu...
Q9RD00 358 eSPLGVRQLIGDVWEWTSSDFTpypgfrafpykeysevFFGPEYKVLRGGSFAVDA---VACRGTFR-NWdYPVRRQIFS 433 Streptomyces coelicolor
P0DPS7 531 -SILGLYDMAGNVYEWCQDWYSydfyessalepdaplgPPQGVYRVLRGGCWKs---LKDDLRCAHR-HRnNPGAINSTY 605 Chlamydia trachomatis...
6MUJ_A 303 GFRCAN 308 Streptomyces coelicolor A3(2)
Q9KYH7 237 GLRLAA 242 Streptomyces coelicolor
Q9KKM6 598 GFRVLR 603 Vibrio cholerae
5HHA_A 279 GFRVAR 284 Pseudomonas aeruginosa PAO1
Q9PFC6 614 GFRLVR 619 Xylella fastidiosa
O34722 461 GIRCVK 466 Bacillus subtilis subsp. subtilis str. 168
P72841 405 GFRCAS 410 Synechocystis sp. PCC 6803
O69671 418 GVRLAW 423 Mycobacterium tuberculosis H37Rv
Q9RD00 434 GFRTAR 439 Streptomyces coelicolor
P0DPS7 606 GFRCAK 611 Chlamydia trachomatis D/UW-3/CX
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