3J16,3MCA,5EO3,3OBY,3WXM,3OBW,2QI2,3VMF,3E20


Conserved Protein Domain Family
eRF1_3

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pfam03465: eRF1_3 
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eRF1 domain 3
The release factor eRF1 terminates protein biosynthesis by recognising stop codons at the A site of the ribosome and stimulating peptidyl-tRNA bond hydrolysis at the peptidyl transferase centre. The crystal structure of human eRF1 is known. The overall shape and dimensions of eRF1 resemble a tRNA molecule with domains 1, 2, and 3 of eRF1 corresponding to the anticodon loop, aminoacyl acceptor stem, and T stem of a tRNA molecule, respectively. The position of the essential GGQ motif at an exposed tip of domain 2 suggests that the Gln residue coordinates a water molecule to mediate the hydrolytic activity at the peptidyl transferase centre. A conserved groove on domain 1, 80 A from the GGQ motif, is proposed to form the codon recognition site. This family also includes other proteins for which the precise molecular function is unknown. Many of them are from Archaebacteria. These proteins may also be involved in translation termination but this awaits experimental verification.
Statistics
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PSSM-Id: 397503
Aligned: 34 rows
Threshold Bit Score: 98.392
Threshold Setting Gi: 305677863
Created: 11-May-2020
Updated: 7-Aug-2020
Structure
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Program:
Drawing:
Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
3J16_A                        277 YSKEIMVMDEFLLHLNKDDDKAWYGEKEVVKAAEYGAISYLLLTDKVLHSDNIaQREE---------------------- 334 baker's yeast...
Q97W96                        275 YIEVEKLLEELKYHLAKDDGLIIYGKEQIKKAMEIGAIEAIVIHEDSTDKE----------------------------- 325 Saccharolobus solfataricus P2...
Q9BMM3                        280 FIREKKVMSKFFEEVAQDTKKYCYGVEDTMKSLLMGAVEVILLFENLNFTRYV-LKNPttgiektlyltpeqeenh---- 354 Blepharisma americanum...
O96203                        276 FIQEKKLIGKFFEEIAQDTGKVVYGIDDTLKALEIGAVELLILYEGLDIIRLT-TKNPvtnqtktmhispcdekqe---- 350 Plasmodium falciparum 3D7...
WGS:AAGF:cds.TTHERM_00292170A 279 FVQEKNVISKFFDCIAIDSGTVVYGVQDTMQLLLDGVIENILCFEELTTLRVT-RKNKvteqithifippnelnnp---- 353 Tetrahymena thermophila SB210...
Q9BMX1                        276 FVEEKNLISKYFEQIALDTGMIVFGVEDTLHSLEIGALDLLMCFENLEINRYE-IRDSandeikiynlnkeqekdpk--y 352 Euplotes aediculatus...
Q9BMM1                        285 FVAEKKLVSKFFEEISLDTGMIVFGVQDTMKALELGAVETILLFEELEITRYV-IKNPvkgdtrtlflnptqqkdsk--y 361 Stylonychia mytilus...
O16520                        288 FIQEKKLIGGYFDEISQDTGKYVFGVKDTLAALEMGAIETLICWENLDIVRYK-MKNSlgedillnlrpdeekdksh--- 363 Caenorhabditis elegans...
CAP68859                      281 FIQEKKLIGKYFEEISQDTGRICYGVEDTLKALELGAVETLIVFENLEVTRWV-LKDSngaeiiihstkqqdaanrd--r 357 Podospora anserina S mat+...
3E20_H                        285 YVQEKKLIQRFFDEISLDSGKYCFGVVDTMNALQEGAVETLLCFADLDMIRYE-FKNSegnpvitymtkeqeekdstnsf 363 fission yeast...
3J16_A                        335 ------------------YLKLMDSVESNGGKALVLSTLHSLGEEL-DQLTGIACILKYPL 376 baker's yeast
Q97W96                        326 ------------------LEKLAQDAENYGVKVFVVGDEVPEAEWVkKTFNGIVGKLRYRL 368 Saccharolobus sol...
Q9BMM3                        355 dnfiengeelealekgplPEWIVDNYMKFGAGLEFITDRSQEGAQFvRGFGGLGAFLRYQV 415 Blepharisma ameri...
O96203                        351 slykennvelevvekislTDWVIGNYKKYGASLDFVTNKSQEGAQFqKGFGGFGGMLRYKI 411 Plasmodium falcip...
WGS:AAGF:cds.TTHERM_00292170A 354 khfkdgehelekievenlTEWLAEHYSEFGAELYFITDKSAEGCQFvKGFSGIGGFLRYKV 414 Tetrahymena therm...
Q9BMX1                        353 fknektgadleiiksgplSEWLCENYSKFGIKLEFITDKSQEGFQFvNGFGGIGGFLRFKL 413 Euplotes aediculatus
Q9BMM1                        362 fkdqasgldmdvisedqlAEWLCHNYQNYXAQVEFITDKSQEGYQFvKGFGGIGGFLRYKV 422 Stylonychia mytilus
O16520                        364 ftdsetgqdmeiietmplLEWFANNYKNFGAALEIVTDKSQEGAQFvRGFGGIGGLLRYRV 424 Caenorhabditis el...
CAP68859                      358 fmdketgqemevvsqesfLEWIAEHYKDFGTTLEFVSDRSTEGNQFvKGFGGIGGILRYKV 418 Podospora anserin...
3E20_H                        364 lldkdtgaemelvssmllSEWLAEHYKDYGANLEFVSDRSQEGMQFvKGFGGIGAVMRYQL 424 fission yeast
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