6S7O


Conserved Protein Domain Family
DDOST_48kD

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pfam03345: DDOST_48kD 
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Oligosaccharyltransferase 48 kDa subunit beta
Members of this family are involved in asparagine-linked protein glycosylation. In particular, dolichyl-diphosphooligosaccharide-protein glycosyltransferase (DDOST), also known as oligosaccharyltransferase EC:2.4.1.119, transfers the high-mannose sugar GlcNAc(2)-Man(9)-Glc(3) from a dolichol-linked donor to an asparagine acceptor in a consensus Asn-X-Ser/Thr motif. In most eukaryotes, the DDOST complex is composed of three subunits, which in humans are described as a 48kD subunit, ribophorin I, and ribophorin II. However, the yeast DDOST appears to consist of six subunits (alpha, beta, gamma, delta, epsilon, zeta). The yeast beta subunit is a 45kD polypeptide, previously discovered as the Wbp1 protein, with known sequence similarity to the human 48kD subunit and the other orthologues. This family includes the 48kD-like subunits from several eukaryotes; it also includes the yeast DDOST beta subunit Wbp1.
Statistics
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PSSM-Id: 397430
Aligned: 113 rows
Threshold Bit Score: 345.309
Threshold Setting Gi: 209556597
Created: 20-May-2020
Updated: 7-Aug-2020
Structure
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Program:
Drawing:
Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
6S7O_G                    47 TLVLLDNLN-VRETHSLFFRSLKDRGFELTFKTADD--PS---LSLIKYGEFLYDNLIIFSPS----VEDFGGNI-NVET 115
XP_002172335              24 vLAIFDHEHaD---YESLLDSLRERDFDVTVKDVKA--PD---SSLFEFGERKYDDLLLFSAR----NKGLGASF-SPKA 90  Sch...
XP_013023469              26 AVADHSLED-----YSTFLNSLTENGYDVTTANVRD--EK---SQLFQYGERMYDNIILLSSQ----SKSFGATF-SPKA 90  Sch...
EFC42032                  40 VLVVVESTQ-IKTTHSRLFDELKKAGFSVEIKLAFD--S----ISLIKYGENVYDHLLIMAPK----AKRLGGDLdDPRK 108 Nae...
KJE89475                  30 TLVLLENYS-LRQTHSTFFRQLQEQDFTLSFALADE--KK---LSLSSYGDRIYDNLIIFAPS----AEEFGGSL-TVRS 98  Cap...
CBJ28067                  43 TLVLADGPQ-IQDSHSMFFSALEARGHELAFRFAGS--AD---VPLTSFGEREFDNLVLLAQSlsgaVEKGSKAV-TVAD 115 Ect...
EEA06642                  47 VLTNVDNIEkIEHRFSDFFKELRYKGHNITFQSLTTk-SRpqdIKLDLYGKMKYNNIIIFDLIepeyKSTFSMN--LLKH 123 Cry...
WGS:AAFB:cds.EHI_028900A  17 TLVILERNE-DQQKFSQFIELLKKNGDEVEII------NKmtlFQLFENGEKKYSNVILLTPH-----YTFKKV--SVKE 82  Ent...
Q23AP5                    32 VIVVVDSSS-YQHTHSTFFNDLRSKGYDLDFRALNS--AT---IKLLTYGEYNYDQMIVIASS-----HMDGKGL-TKNK 99  Tet...
EGF79688                  33 TLVLLDDLA-NRSEYSIFLDSLTARGHRLFVHTVAQisTT---VPLLVYGDLAFSHVVILASK----VSTFGGTV-GVGG 103 Bat...
6S7O_G                   116 ISAFIDGGGSVLVAASSDI-------GDPLRELGSEC-GIEFDEEKTAVIDHHNYD-ISDL--------GQHT----LIV 174
XP_002172335              91 LIEFVEDGGNILLAAGTNV-------PEGLREFGRQL-GIFVAERGNALVDHFAS--------------VGSEdhqlVAL 148 Sch...
XP_013023469              91 ILEFTQAGGNVLFAAGSNL-------PEGIRELGRQL-DIYIGERHSSVVDHFHS--------------VESSndesVLL 148 Sch...
EFC42032                 109 ILEFIDRGHNVFIVASEDGl------TQNLRKVAIES-GIELDDDGTNVIDHFNYDvKLDS--------DKHT----TIV 169 Nae...
KJE89475                  99 ILDFVDHGGNVIVAGSSSI-------GEPIREFGSEC-GFEFDEESAAVIDHINFDsKLDN--------GEHT------- 155 Cap...
CBJ28067                 116 IMEFIEDGGNVLMAGGDSM-------GTLSRSVGALC-GVDFDPQGSRVVDHFTA--VKDEqlgelegrGVYA------- 178 Ect...
EEA06642                 124 LLDFVDNGNNILVFTDTSDksnsiipTPMIRRFAKECaEIEFHPSNSYLIDIVGNQs------------NEYN----QNC 187 Cry...
WGS:AAFB:cds.EHI_028900A  83 FIQFIDNGGNMVITLGKKY-------EDGYKQLLYSL-DMEVDSNGSNVVD------------------EKHT----VKI 132 Ent...
Q23AP5                   100 IAEFFDSGRNVFIATDIDS-------SKTIRQLYNEF-GVDIDEYSSRVQDHFNKDsEQDHsyvyskniIKQE----VFC 167 Tet...
EGF79688                 104 LIDYVNAGGNVLLAASSEL-------SEVVRDFAYEF-SVDFDESGTSVFDHFN---------------SIHDspsqIIS 160 Bat...
6S7O_G                   175 --ADT---ENLLKAPTIVGk----SSLNPILFRGVGMVADPd-NPLVLDILTGSSTSYSFFPDKPITQYPH------A-V 237
XP_002172335             149 --------DEFSSSKYIVSde--tRNAGAVLYKGSGHALGE--NPLVEPVLRAKTTSYLYNQKSESESSAHp----wA-A 211 Sch...
XP_013023469             149 --------DAVSENPYIISde--tRQAGSIVYKGNGHYLGT--NHRAQPVLRGHPSTYVYNKKSEGKVSEDp----wA-A 211 Sch...
EFC42032                 170 --IKDe-kQFACKKTNILGsalskFSTQPVLFRGLGMLLKPq-NKLLVPILSGASTSYSYST-SDERVQTEp----lV-K 239 Nae...
KJE89475                 156 --TIVt--dQIIDNKLIAGt-----NKGRVLFRGVGLVQDLs-NPLTIRVLSAESTAYTYFTDEKISVAPHv-------V 218 Cap...
CBJ28067                 179 --NTAikgEVAADNALYLGsysagRDGA-VVFSGVGHEVDDe-NYLATKVLTASGTAYSADPTNSIGKFPEn-------A 247 Ect...
EEA06642                 188 --ISSpncL---DSKAIFSpdtcnGKE--ITYCGIGFAIKSylTPLSFPLLRATDTTMAFLTNSATRNNKllgnllyS-S 259 Cry...
WGS:AAFB:cds.EHI_028900A 133 geFEMifsNNVHNNQNIFNqri-----qnILFSGIGLYLPP--SPFTSSLLKAQDSA-STSLFPNVSFAQEt-------- 196 Ent...
Q23AP5                   168 pqLDKp-----------------------ILYRGAGLTMTPyeTYQVYGVLRGEDTTYSYSQERRESVNS---------- 214 Tet...
EGF79688                 161 s-------SFTNKTNFILSes--tRQGPPVVFQGVGHKVTGk-NILTFPILTASHSAYSYDSKDTDPLLGg------PlL 224 Bat...
6S7O_G                   238 GK-NTLLIAGLQARNNARVIFSGSLDFFSDSFFNSAVQKaa----pgSQRYSQTGNYELAVALSRWVFKEEGVLRV-GPV 311
XP_002172335             212 GS-QLFVVSSLQTLNGSRVAFSSSLDIFKNEYLSPSSE----------S--YNAANALFARDLTNWVFQRSGRLVL-ENM 277 Sch...
XP_013023469             212 GT-QLFLVSVFQNDAGERIGFSGSPEMFTNTYLSPNSP----------S--YSAANALFARDLTNWVFQRVAVLKA-SDM 277 Sch...
EFC42032                 240 GR-ETVLIGANQARNGARLTICGSMDLLSNQFFDA--SItqfsldgnAKTFAASGNALFSIELIRWAFQSRGLLRH-TKV 315 Nae...
KJE89475                 219 GS-KTVLVGALQARNNARVLFSGSLDLFSDEFFNAQVNVa-------GATASPSANRDFAISAALWTFKRSGILRVsGEP 290 Cap...
CBJ28067                 248 GR-DTLLVSAVQARTNARMLFTGSLAMFSNRFF----------------TLPEAGNNVFCSEISKWVFAERGILRA-SSI 309 Ect...
EEA06642                 260 GNnEIGLVVSMQARNGARVTFSGDGYLCSNDRYMI-----------------NGNNQLFCNDIASWTFQKRGLIKL-SNI 321 Cry...
WGS:AAFB:cds.EHI_028900A 197 ---NITLVASLQARNNARIIVSGSSLLFSNIAFDSVIEHp-------SLNLIKSDNKKFTENIIDWVLQRRCVIRM-KNI 265 Ent...
Q23AP5                   215 GS-QVILVSATQGRNNARAVLTGSIEMLSNEFY----------------KNEKYANRQFVNNVIDWNFGLNGVLKV-GKI 276 Tet...
EGF79688                 225 GE-TISLASAFQARNNARVVFVGSTSLFSNKSI--------------------CGNAAFSADISKWVFQEKSVVQI-TNT 282 Bat...
6S7O_G                   312 SHH-RV-----------G-E-----------T--AP---PNAYTVTDLVEYSIVIQQLSNG-K--WVPFDGDDIQLEFVR 359
XP_002172335             278 TYGlISe--------------------------sVEernGPVYRIKDEMEFTADMSVYENGvR---KPYIASDVQMELVM 328 Sch...
XP_013023469             278 TYG-KV-----------Ge--------------pYElrnASTYRIKDQMVFSIDIAILNDG-Q--PQPYVADDVQMELVM 328 Sch...
EFC42032                 316 HHY-LAdd--------vT-Kt---------qP--------TAYRVGQELTYTVSIEEWNGR-D--WVPYVTDDIQLDFIM 365 Nae...
KJE89475                 291 KHH-LA-----------Gq--------------tTV---PELYTVFDQIEYSLRVEELVDR-N--WVGYQGNDMQLEFVM 338 Cap...
CBJ28067                 310 THT-RQdgsdaelllkqKeRrdlpmslfpepEiaKN---SQVYRVKDNVTYSVVIEEFDSE-KggWRPYTAEDVQMEFVM 384 Ect...
EEA06642                 322 QSH-KIedi-----snsR-Pisr------ysDrkDA---DVEYTVEDFINFSAEFYHNIND-K--WEPYPYNDIQLELTM 382 Cry...
WGS:AAFB:cds.EHI_028900A 266 HWE-KIn----------G-V-----------KevDY---DHQLVINDTIKVNVELEQLNQG-N--YVPFNVDDLQIEFKL 316 Ent...
Q23AP5                   277 TYT-RK-----------Ge--------------kEN---SNEYRIKDFLDYSIEIFTYSQK-KnsWVPYITDDIVLEFIM 326 Tet...
EGF79688                 283 KHH-RV-----------Ne--------------tDQ---HGIYRIKDEMIYSATIKVWSGD-A--WEPFVANDIQFEAVM 330 Bat...
6S7O_G                   360 IDPFVRTFLKKKg------GKYSVQFKLPDVYGVFQFKVDYNRLGYTHLYSSTQVSVRPLQHTQYERFIPSAYPYYASAF 433
XP_002172335             329 LDPYYRLNFEPVesdlkdaQRYSVKLVAPDHYGVFSYKVRYSRPGLTNIDEKQTFTLRQFHHNEYPRFLPHAYPYYTSAF 408 Sch...
XP_013023469             329 LDPYYRVNMVPVasdsktyQHYEAVLTAPDHYGVFTYKIAYNRPGLNPIDEKATFTIRQFYHNEYPRFLPHAYPYYVSCF 408 Sch...
EFC42032                 366 LHPYQRVFLKFDeka----qkYSATLKAPDTYGVFKFVVDHRRHGFNQLIFADQVTVRPMHINEFERFVPAAYPYYTSVF 441 Nae...
KJE89475                 339 LNPYLRLTLNNTg-----dGVQHASFQAPDVYGVFQFRVDYKRTGYTYVQVHNQIPVRPLQNTQYERFIFSAFPYYASAF 413 Cap...
CBJ28067                 385 LDAYERITLEPga-----rGLFKKTFTVPDTYGIFKFRVHYRRPGYTVLSFSTQVSIRPFTHSEYERFLVAAYPYYASAL 459 Ect...
EEA06642                 383 LNPYIRIGLQHKgdgdishATFHTNIKLPEVFGIYKFVINHKRIGYNYILYESLVTIRSFRHDQNQRLLISAYPFYTSFL 462 Cry...
WGS:AAFB:cds.EHI_028900A 317 LDPVIVKNFKRid-----nGKYEVIVQTPDKFGVYTMIINYRRPFLSYLEYKETIPLRTFRLTQVDRFLTGAYPFYAACA 391 Ent...
Q23AP5                   327 LDPYIRQTLTREgk----tQVYKASFQIPDKHGIFKLKVKYQKPGFTFIEESNKVTVRPFKHNEYPRYLEQAYPYYFSTW 402 Tet...
EGF79688                 331 LDPYIRKTMNEs------dGVYVARFTLPDQYGVFSFKLNYQRHGLSYISASETVQVRPFRHNQFPRFLVVAYPYYANIF 404 Bat...
6S7O_G                   434 SMMLGLFIFSIVFLHMKEKEKS 455
XP_002172335             409 IVFVGFVMFAGAWLFFKPAAPS 430 Schizosaccharomyces japonicus yFS275
XP_013023469             409 AVLGAFLIFCGIWLVQIPKASS 430 Schizosaccharomyces cryophilus OY26
EFC42032                 442 VMMGAFVVFVIVLTFTAEPKDK 463 Naegleria gruberi
KJE89475                 414 SMMGGLFVFSLVFLYHREAPAT 435 Capsaspora owczarzaki ATCC 30864
CBJ28067                 460 SVMVGFLMFCAAFLYSKDEAPS 481 Ectocarpus siliculosus
EEA06642                 463 LTLTLFIFFVIIFVFVIDNSKK 484 Cryptosporidium muris RN66
WGS:AAFB:cds.EHI_028900A 392 SMAVGFIVFSFIYLNQIEKKei 413 Entamoeba histolytica HM-1:IMSS
Q23AP5                   403 ATLIGFLVLVVYFLFHQDKEVS 424 Tetrahymena thermophila SB210
EGF79688                 405 SLMVAFFLFSAVFLFNKEVRPK 426 Batrachochytrium dendrobatidis JAM81
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