5NEN,5NG5,6TA6,3OOC,4TKO


Conserved Protein Domain Family
CusB_dom_1

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pfam00529: CusB_dom_1 
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Cation efflux system protein CusB domain 1
The cation efflux system protein CusB from E. coli can be divided into four different domains, the first three domains of the protein are mostly beta-strands and the fourth forms an all alpha-helical domain. This entry represents the first beta-domain (domain 1) of CusB and it is formed by the N and C-terminal ends of the polypeptide (residues 89-102 and 324-385). CusB is part of the copper-transporting efflux system CusCFBA. This domain can also be found in other membrane-fusion proteins, such as HlyD, MdtN, MdtE and AaeA. HlyD is a component of the prototypical alpha-haemolysin (HlyA) bacterial type I secretion system, along with the other components HlyB and TolC. HlyD is anchored in the cytoplasmic membrane by a single transmembrane domain and has a large periplasmic domain within the carboxy-terminal 100 amino acids, HlyB and HlyD form a stable complex that binds the recombinant protein bearing a C-terminal HlyA signal sequence and ATP in the cytoplasm. HlyD, HlyB and TolC combine to form the three-component ABC transporter complex that forms a trans-membrane channel or pore through which HlyA can be transferred directly to the extracellular medium. Cutinase has been shown to be transported effectively through this pore.
Statistics
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PSSM-Id: 425733
Aligned: 43 rows
Threshold Bit Score: 114.443
Created: 21-Mar-2022
Updated: 13-Oct-2022
Structure
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Program:
Drawing:
Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
5NEN_B        23 FTRLDKGVASPGSVTVSGNRKT-VQAPASGIIKNIA-VRDGDKVKAGEVLVQLSQVQAQAQVDSLRDQYYTTLATEGRLL 100 Serratia marces...
Q9ZNG9        43 PAPIGITSELPGRLEAYRQAE--VRARVAGIVTRRL-YEEGQDVRAGTVLFQIDPAPLKAALDISRGALPGRGQPRAAAD 119 Pseudomonas aer...
WP_004538316  47 KTSVPLSVELPGRLDAYRQAE--VRARVAGIVTART-YEEGQEVKRGAVLFRIDPAPFKAARDAAAGALEKAQAAHLAAL 123 Burkholderia ps...
5NG5_G        23 TEPLQITTELPGRTSAYRIAE--VRPQVSGIILKRN-FKEGSDIEAGVSLYQIDPATYQATYDSAKGDLAKAQAAANIAQ 99  Escherichia coli
P37636        43 PGSVNVLSELPGRTVPYEVAE--IRPQVGGIIIKRN-FIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNAR 119 Escherichia col...
KEX94207      46 AQALTLTTDLPGRTSAFRVAE--VRPQVSGILQKRS-FVEGAEVKLGQQLYQIDPRTYEAQLRRAEANRTSAQNLARRYE 122
6TA6_G        22 AQTVTLNTELPGRTNAFRIAE--VRPQVNGIILKRL-FKEGSDVKAGQQLYQIDPATYEADYQSAQANLASTQEQAQRYK 98  Pseudomonas aer...
P37973        80 SAAVGTEILAPATVAALPGSEAvIVSRAAGAVQRVQ-RRLGDVVKAGDVLALVDSPEAAGMAAERKVAQAKADLARKTYE 158 Cupriavidus met...
Q8CWA2       101 RGPLTFSQSFPANVSYNEYQYAiVQARAAGFIDKVYpLTVGDKVQKGAPLLDLTIPDWVEAQSEYLL-LRETGGTATQTE 179 Escherichia col...
3OOC_A       101 RGPLTFAQSFPANVSYNEYQYAiVQARAAGFIDKVYpLTVGDKVQKGTPLLDLTIPDWVEAQSEYLL-LRETGGTATQTE 179 Escherichia col...
5NEN_B       101 AERDGLSIvtfspildavkdkprvaeiialqtqlfasrrqaLQSEIDGYKQSMDGIRFQLKGLQDSR-GNKQIQLSSLRE 179 Serratia marces...
Q9ZNG9       120 KLKAYADL----------------------------------IKDRAISEREYTEAQTDARQALAQI-ASAKAELEQARL 164 Pseudomonas aer...
WP_004538316 124 DKRRRYDE---------------------------------LVRDRAVSERDHTEALADERQAKAAV-ASARAELARAQL 169 Burkholderia ps...
5NG5_G       100 LTVNRYQK---------------------------------LLGTQYISKQEYDQALADAQQANAAV-TAAKAAVETARI 145 Escherichia coli
P37636       120 ITFNRQAS---------------------------------LLKTNYVSRQDYDTARTQLNEAEANV-TVAKAAVEQATI 165 Escherichia col...
KEX94207     123 TLLKTKAV-----------------------------------------------SKQQYDDALAAW-KQAEADYQVARI 154
6TA6_G        99 LLVADQAV-----------------------------------------------SKQQYADANAAY-LQSKAAVEQARI 130 Pseudomonas aer...
P37973       159 REASLFQQ---------------------------------------------------------GVtPRQEMEAAKAAL 181 Cupriavidus met...
Q8CWA2       180 GILERLRL--------------------------------------------------------AGMpEADIRRLIATQK 203 Escherichia col...
3OOC_A       180 GILERLRL--------------------------------------------------------AGMpEADIRRLIATQK 203 Escherichia col...
5NEN_B       180 QMNSMKQLAADGYLPRNRYLEVQRQFAEVNSSIDETVGRIGQLQKQLlesqqriDQRFADYQREVRTQLAQTQMDASEFR 259 Serratia marces...
Q9ZNG9       165 RLGYATVTAPIDGRARRALVTEGALVGEDSPTPLTRVEQIDPIYVN------------FSQPAAKSRHAAGDPRRPGEGC 232 Pseudomonas aer...
WP_004538316 170 QLDYATVTAPIDGRARRALVTEGALVGQDQATPLTTVEQLDPIYVNF-------SQPAADVESLRRAVKSGRAAGIAQQD 242 Burkholderia ps...
5NG5_G       146 NLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDV-------TQSSNDMMR-LKQELANGTLKQENGK 217 Escherichia coli
P37636       166 NLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDL-------TQSVQDFLR-MKEEVASGQIKQVQGS 237 Escherichia col...
KEX94207     155 DVQYTRVLSPISGRIGRSTVTEGALVTNGQAQSLATVTQLDPIYVDV-------TQPITKLLGLQKALESGRLQKTGENQ 227
6TA6_G       131 NLRYTKVLSPISGRIGRSAVTEGALVTNGQANAMATVQQLDPIYVDV-------TQPSTALLRLRRELASGQLERAGDNA 203 Pseudomonas aer...
P37973       182 DVAQAEALRAATVAQSAHLASDGRSVAVVSPIAGKITAQSVTLGAF--------VAPQAELFRVAGTGAVQVEAAVTAAD 253 Cupriavidus met...
Q8CWA2       204 IQTRFTLKAPIDGVI--TAFDLRAGMNIAKDNVVAKIQGMDPVWV---------TAAIPESIAWLVKDASQFTLTV---- 268 Escherichia col...
3OOC_A       204 IQTRFTLKAPIDGVI--TAFDLRAGMNIAKDNVVAKIQGMDPVWV---------TAAIPESIAWLVKDASQFTLTV---- 268 Escherichia col...
5NEN_B       260 NKLQMADFDlGNTAIT-SPVDGTVVGLNIFTqGGVVGAGDHLMDVVPsqATLVVDSRLKVDLFDKVYNGLPVDLMFTAFN 338 Serratia marces...
Q9ZNG9       233 RRQGHRRAPgPGRRQR-VPLAGELLFIDLAVdPGTDTIAMRALFRNP--HRELLPGGYVQVRLQRAVNPQAITVP-DALI 308 Pseudomonas aer...
WP_004538316 243 VEVTLVRPDgSTYARK-----GKLLFADLAVdPSTDTVAMRALFPNP--ERELLPGAYVRIALDRAVARDAILVPRDALL 315 Burkholderia ps...
5NG5_G       218 AKVSLITSDg----IK-FPQDGTLEFSDVTVdQTTGSITLRAIFPNP--DHTMMPGMFVRARLEEGLNPNAILVPQQGVT 290 Escherichia coli
P37636       238 TPVQLNLENgKRYSQT-----GTLKFSDPTVdETTGSVTLRAIFPNP--NGDLLPGMYVTALVDEGSRQNVLLVPQEGVT 310 Escherichia col...
KEX94207     228 AEVSLTLDDgSAYPLP-----GTLKFSEVSVdPTTGSVTLRAEFPNP--NRKLLPGMFVHALLKEGVQNAAILVPQQAIS 300
6TA6_G       204 AKVSLKLEDgSQYPLE-----GRLEFSEVSVdEGTGSVTIRAVFPNP--NNELLPGMFVHAQLQEGVKQKAILAPQQGVT 276 Pseudomonas aer...
P37973       254 TSRIVAGSEaTILLANgSPLSARVQAVTPTVtGSARVATVVVVPAQP--TDRLVVGEGVQVRLRTAVADAAALSVPEDAV 331 Cupriavidus met...
Q8CWA2       269 ----PARPDkTLTIRKwTLLPG--------VdAATRTLQLRLEVDNA--DEALKPGMNAWLQLNTASEPM-LLIPSQALI 333 Escherichia col...
3OOC_A       269 ----PARPDkTLTIRKwTLLPG--------VdAATRTLQLRLEVDNA--DEALKPGMNAWLQLNTASEPM-LLIPSQALI 333 Escherichia col...
5NEN_B       339 QNKTPKIPGTVTLVSAD------RLVDKANGEPYYQMQVTVSPEGMKML 381 Serratia marcescens
Q9ZNG9       309 RT---AQSAVVKVVNPKglvedvEVRADTLQGRDWIISRGLKGGEWVIV 354 Pseudomonas aeruginosa
WP_004538316 316 RTAD-SATVKVVGQNGKir--dvTVEAAQMKGRDWIVTRGLAGGERVVV 361 Burkholderia pseudomallei
5NG5_G       291 RTPRGDATVLVVGADDKve--trPIVASQAIGDKWLVTEGLKAGDRVVI 337 Escherichia coli
P37636       311 HNAQGKATALILDKDDVvq--lrEIEASKAIGDQWVVTSGLQAGDRVIV 357 Escherichia coli K-12
KEX94207     301 RDTRGVPSVWVVKADNTve--srEIQTLRTVGNAWLISNGVTEGERIIT 347
6TA6_G       277 RDLKGQATALVVNAQNKve--lrVIKADRVIGDKWLVTEGLNAGDKIIT 323 Pseudomonas aeruginosa
P37973       332 QNLDGRDVLFVRTQEGFrp---mPVLVGTRSGGSAQILSGVQAGEQVAT 377 Cupriavidus metallidurans CH34
Q8CWA2       334 DT---GNEQRVITVDADgrfvpkRVAVFQASQGVTALRSGLAEGEKVVS 379 Escherichia coli CFT073
3OOC_A       334 DT---GSEQRVITVDADgrfvpkRVAVFQASQGVTALRSGLAEGEKVVS 379 Escherichia coli K-12
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