Conserved Protein Domain Family
B12_rSAM_KedN5
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NF033712: B12_rSAM_KedN5 
KedN5 family methylcobalamin-dependent radical SAM C-methyltransferase
KedN5, the founding member of a family of radical SAM enzymes with an N-terminal B12-binding domain, is a C-methyltransferase that relies on a methylcobalamin cofactor during natural product biosynthesis.
Links
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Statistics
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PSSM-Id: 411301
Aligned: 37 rows
Threshold Bit Score: 1025.23
Created: 20-Oct-2021
Updated: 25-Oct-2021
Structure
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Aligned Rows:
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
WP_051624190  23 IPLAAGYLKSMAYDNQYIKSNIDIDICNFNGSAETISIIRDMFAEQHVEIACFSMFGWNYNQFANVADTFKQLNPKGWVI 102 Clostridium akagii
WP_026189966  19 MPLAVGYLKAVVDETPSFAGEATAEICNFRGGQRMQEMTKALFSGGIPDVLAFSVLGWNYRNFGVLSETFKQVNPNGLVV 98  Salinispora moo...
WP_122195388  19 MPLASGYLKATLDADQDIAAEIDVRICNFRGGTGLTNMARDLFSEAVPDIIGFSVLGWNYRSFACLAETFKQLNPAGLVV 98  Actinomadura ha...
WP_019057203  19 MPLAAGYLKAVLDADPDIGREVDVRIHNMRGGWSVNEMTRTIFHDEVPDVLAFSVVGWNYRNFSLLAEVHKQLRPDGLTI 98  Streptomyces pr...
BAI23333      19 MPLAAGYLKAVLDADPGIGPEVDVRIHNMRGGWPVNQMTRAIFSEEVPDVLAFSVVGWNYRNFSLLADVHKQLKPDGFTV 98  Streptomyces gr...
WP_042183769  19 MPLAAGYLKAIIESDSDLAGSAGVDICNFRGGMSLTEMAARLFTGVVPDMLAFSVLGWNYRNFLCLAETYKQINPRGVVV 98  Kibdelosporangi...
WP_010314446  22 MPLAAGYLATMTR--EQLGEHCDVRIFNASGSMTPLEMAVDILRDGVPDVIGFSVLGWNVRQFASVAETVKQANPEAVVV 99  Saccharopolyspo...
PPK65224      22 MPLAAGYLAAVVRAHPELGSRCAVRILNFPGHLSPLEMAIKVLQAGVPDVIGFSVLGWNANQFAAVAATVKQVNPSAVVV 101 Actinokineospor...
SFD85079      19 MPLAAGYLAASVESDTRLREECDISIENFPGDVTPIEMAIQLLRGGVPDVVGFSVLGWNVRQFSAVAESIKQANPECVIV 98  Actinopolyspora...
WP_014748295  18 MPLAAGYLAGCIRADPVLATGCEVSIANFSGTVTPLEMAVRLLEDGVPDLVGFSVLGWNLRQFSAVAETLRQVDPGVTIV 97  Tistrella mobilis

WP_051624190 103 FGGTHVTNQATKVFKKYPAVDIIINGEGELTFCEVLLAYLNNK---SISELSYIKGISFRnEDREIVTTEPRERIKNLDI 179 Clostridium akagii
WP_026189966  99 FGGNHVAYQAERVFRELPWVDVVVNGEGEHTFGELLTYLLERPdgsNVFDPTHVAGLSFRrADGTLHSTPDRDRIAHLDV 178 Salinispora moo...
WP_122195388  99 FGGNHVAHQAARVFREHPQVDVVVNGEGEHTMLDLVRYLLTEN---GDFDPHEVAGISYKrPDGTEHTTAARERIENLDI 175 Actinomadura ha...
WP_019057203  99 FGGNHVSHQAPKVFRECPSVDIVVNGEGELTFRELISSVLADR---RDPNLPPISGISFRtDGTDFITTPDRDRINDLDI 175 Streptomyces pr...
BAI23333      99 FGGNHVSHQAKKVFRECPDVDIVVNGEGELTFRELVTAILANR---HTPDFTDVAGLSFRlSGDEYFTTPDRARIDDLDI 175 Streptomyces gr...
WP_042183769  99 FGGNHVAYQAGRVFRESAAVDLVVDGEGELTFRDLVGRLADSP---HAPDFTGIAGLSHRdRDGAACQSGDRERVSDLDI 175 Kibdelosporangi...
WP_010314446 100 FGGNHVANQSERVLRRDVAVDVVVNGEGEITFVELLRAVLEGT------SYANVRGISYRgEDGSIVTTEEQPRLDDLDV 173 Saccharopolyspo...
PPK65224     102 FGGNHVAKQAARVLTRDPAVDIVVNGEGEVTFVNILKAVLDGG------PYARVRGISFRgPDGSVVTTADEARVDDLDR 175 Actinokineospor...
SFD85079      99 FGGNHVAHQATRVFNNCDAVDVVVNGEGEFTFRDLLHALLDER------NYSEIDDISFHdTDGQLVTTPARDRITDLDE 172 Actinopolyspora...
WP_014748295  98 FGGTHVANQAARIFRLCPEVDIVVNGEGEFAFTEIVRARL------GMMPLGDVAGISFRdGAGDVVTTPERPRILNLDA 171 Tistrella mobilis

WP_051624190 180 IPSPFLTGAIDLTNEEGKFKYDVALIETNRGCPYKCGYCFWGGATGQKIYNFSIERLRAEIELFAKLKVNDICLCDSNFG 259 Clostridium akagii
WP_026189966 179 IPSPLLSGAIPMTDASGQFRYDVALLETNRGCPYKCAFCYWGGAVGQKMRSFSIDRLTAELDVFGYYKAPSLVLCDSNFG 258 Salinispora moo...
WP_122195388 176 IPSPFLTGTLPMLDAAGNFRYDVALMETNRGCPYKCSFCYWGGAVGQRMRSFSRERLAEELDFFAFHESPTIVLCDSNFG 255 Actinomadura ha...
WP_019057203 176 IPSPFLTGAIPLLDRNGNFPYEFALLETNRGCPYRCSFCYWGGATGQRVRSFSRDRLAAELDLFGYHQVPTVFLCDANFG 255 Streptomyces pr...
BAI23333     176 IPSPFLTGAIPLLDRSGRFPYEFALLETNRGCPYRCSFCYWGGATGQRVRSFSRDRLAAELDLFGYHQVPTVFLCDANFG 255 Streptomyces gr...
WP_042183769 176 LPSPLLTGAVPLTGDDGRFRYEFALLETNRGCPYKCAFCYWGGAVGQRVRAFSRARLAAELDLLGRNQVHTVFLCDANFG 255 Kibdelosporangi...
WP_010314446 174 IPSPILTGALPLVDERGEFRYDVALMETNRGCPYHCSFCFWGGAVGQKVRAFSRERLRAELEVLAKAKAHTVVLCDANFG 253 Saccharopolyspo...
PPK65224     176 IPSPILTGAVPLLDERGGFRYDVALMETNRGCPYHCAFCYWGGAVGQKVRSFSRVRLRAELEALARAGADTVVLCDANFG 255 Actinokineospor...
SFD85079     173 IPSPILNGSVPLLDDNGRFRYDVALLETNRGCPYHCAFCFWGGAVGQKVRAFSRERLRRELEALARAGAETIVLCDANFG 252 Actinopolyspora...
WP_014748295 172 IPSPILEGVIPLTDDDGAFRYDVALMETNRGCPYHCAFCYWGGAVGQKVRSFSRARLRAELEAVARAGARTVVLCDANFG 251 Tistrella mobilis

WP_051624190 260 LTEHDEQFFDILLEAREKYGYPKCIITSWAKNKTQIFSRILKKVKDSRFMTSFSISLQSLSEDVLKNMGRVNMKRDDWKK 339 Clostridium akagii
WP_026189966 259 LLAADEEFVEALIKTKEKYGYPGDLITSWAKNKSQRFYNIVRELKKHDMHSLFTLALQTLDDAALTDMLRKNMKVNQWES 338 Salinispora moo...
WP_122195388 256 LLEQDEEFVEDLIEVRNRRGYPRALETSWAKNKSARFMRIVSRLRENGFKSSFTLALQTLSDEALTDMQRKNMKVNKWEG 335 Actinomadura ha...
WP_019057203 256 MLEADEEFTEDLLKINEKYGFPTSLEANWAKNKSQRFHRIVRTLKERGFKSSFTLALQTLSDEALTGMQRRNMKLNQWQD 335 Streptomyces pr...
BAI23333     256 MLEADEEFTEDLLKIHEKYGFPVSLEANWAKNKSQRFHRIVRTLKQYGFKSSFTLALQTLSDEALTDMRRRNMKLNQWQD 335 Streptomyces gr...
WP_042183769 256 MLEADEQFVDDLVATRERYGYPRALEANWAKNKSARFHSIVRTLKRQGFKSSFTLALQTLTDEALIDMNRRNMRLNQWED 335 Kibdelosporangi...
WP_010314446 254 MLRQDMEFIEDFIEVRDRYGYPRALETSWAKNKSKVFRDIVRRMREVGLGSSFTLALQTLNDSVLDLMNRRNMKINQWRE 333 Saccharopolyspo...
PPK65224     256 MLRQDAEFVDDLIEIRDRYGCPRALETSWAKNKSAVFRGIVRRMREAGMRSSFTLALQTLDDTALDLVNRRNMKINQWRD 335 Actinokineospor...
SFD85079     253 MLPQDMEFVEDLIAVKSRYGYPVALETSWAKNKNAMFYGIVRLMRDAGLQSSFTLALQTLDVDTLSNMNRRNMKINQWRE 332 Actinopolyspora...
WP_014748295 252 MLHQDMDFVRDLIEIRDRYGFPTALETSWAKNKNATFRDIVRLMKREGLQSSFTLALQTLDDETLGTMNRRNMRINDWKE 331 Tistrella mobilis

WP_051624190 340 VAKDLRKDGLDVYGELIWGLPGETPDSFFKGYDIMSEEVTRIAVYPHLLIPNTKYSMNKNQYGIVGWKAGKDDFEYVLKH 419 Clostridium akagii
WP_026189966 339 LVEWLAEEGLECYGELIWGAPGETVESFLEGYDRLARKVSRIAVYPMLILPNTAYAQNRELHGLVTIRGETDDFEYVLAN 418 Salinispora moo...
WP_122195388 336 LVDWLNDEGLDCYAELIWGAPGETPESFLDGYDRLASRVPRIAVYPLLLLPNTNYVERRDLHGFVTVRGQDDDFEYVLAN 415 Actinomadura ha...
WP_019057203 336 LSDWLADEGMDAFVELIWGAPGETVESFLDGYDRIAEKVPRIAVYPLLLLPNTAYSEQRELHGFVTVRGESDDFEYVLAN 415 Streptomyces pr...
BAI23333     336 LAEWLAAEGMESFVELIWGSPGETVETFLDGYDRIAEKVPRIAVYPLLLLPNTDYSEKRELHGFVTVRGESDDFEYVLAS 415 Streptomyces gr...
WP_042183769 336 LVDWLNEEDLECYAELIWGTPGDTPEQFLAGYDKLAERVPRIAVYPLLLLPNTAYVERRDLHGFLTVRGQHDDFEYVLAS 415 Kibdelosporangi...
WP_010314446 334 LAEWLAAEGLDCYAELIWGVPGETPESFLEGYDELAKHVSRIAAYPLLLLPNTDFSDRRELHGFITVRGQRDDFEYVLSA 413 Saccharopolyspo...
PPK65224     336 LAEWLTAEGLDCYAELIWGIPGETPETFLRGYDELAKHVSRIAAYPLLLLPNTDFSDRRAVHGFVTVRGRQDDFEYVLAA 415 Actinokineospor...
SFD85079     333 LAEWLAAEGLDAYAELIWGAPGESPDSFLAGYDELAKHVSRIAAYPLLLLPNTDYTERRELYGFVTVRGDRDDFEYVLAN 412 Actinopolyspora...
WP_014748295 332 LAAWLSAEGLDVYAELIWGAPGETPASFLRGYDELARHVTRIAAYPLLLLPNTDFTDRRAEHGFITVRGERDDFEYILAN 411 Tistrella mobilis

WP_051624190 420 NTMTFEENCKMHRFIFWARLLAEYSLLRHIWIPIRKIANITQSQVLLNFDNWID--QQSDDTCKMLREYRDEVvkSLDAY 497 Clostridium akagii
WP_026189966 419 RSSTLQENLQMQRFVFWARVLGEQQYLRHAWRPLLDLANMSQSTVITSFKTFFE--ASTDPAAVTLCDRIPV---LAESP 493 Salinispora moo...
WP_122195388 416 RGATIAENLEMQRFIFWARIFGENQYLRHLWRPARDLTGLSQSELVQSLITAAD--RSADAEVAEFRELAPV---IAESP 490 Actinomadura ha...
WP_019057203 416 RTSTLAENFAMQRFMFLARILGENQYFKRLWRPARVLAGMSQSEVIHSLLEWIS--ASDHPSVVAFREAFPV---LAESP 490 Streptomyces pr...
BAI23333     416 RSSTLAENFAMQRFMFLARILGENQYFKRLWHPARLLAGMTQSQVIYSLMEWID--ASEHPSAVAFRGMFPV---LAESP 490 Streptomyces gr...
WP_042183769 416 RSAGLPEHLRMQRFMYLARILGENQYFKRLWAPARLLGGLSQSRIVLSLLDWLD--ASADPAVAAFRESFPV---LAESP 490 Kibdelosporangi...
WP_010314446 414 RGISLEENIRMQRFLFWARLLAENLVLREVWPVFRAVLGWTQSTCILSLAGFVEa-QQGHLGAALLRQAAESS--TADPD 490 Saccharopolyspo...
PPK65224     416 RGVSLAQNLRMQRFLFWARLLAENLVLRDTWPVFDAVLGWSQSAVILSLADFVEaeAAHHAGARLLSAAATSS--TADPD 493 Actinokineospor...
SFD85079     413 KDVSLSENLRMQRFLFWARLLAENVVLRNIFPVLRATGSMTQSEAILSLADYVD--EQNRPGARLLTQAAERS--TADPD 488 Actinopolyspora...
WP_014748295 412 RSAGLHDHLRMTRFLFWARLLAENLVFRGLWPVLRAVAGLSQSAAILSIGGHVE--ASRAAGAEILRQAAHAA--FADPD 487 Tistrella mobilis

WP_051624190 498 RIEDELNYFFKTKKVKEIVNKWWNEEIIKKVPNKYKDFFKELMIYDLLTMPIYGTGTMKISDKsdiqyLdgyPLKKVGND 577 Clostridium akagii
WP_026189966 494 AIAEALRSLHSSPELQALVHQWWTHEIVPVVPPQWQPFITSVFEYERWSRQVYVVPDGDLPDG-----W---HEVDLDGT 565 Salinispora moo...
WP_122195388 491 AVIAGLRRLYAQPRLERFVAHWWEESVVPRFPAAWRRFALDLYDFERWSRPVYVEPAGGPPPG-----W---RETTVADT 562 Actinomadura ha...
WP_019057203 491 AVASGHRLLYSGPELDAEIERWWNETVVPRFPAGWTEFGPYFYRFERLARPVYQPPGAALPDG-----W-------REEG 558 Streptomyces pr...
BAI23333     491 AVAAGHRMLYSDPELDAEIERWWNEQVVPRFPAAWAEFGPHLYRFERLSRPVYQPPGGSVPDG-----W-----RLVEGG 560 Streptomyces gr...
WP_042183769 491 AVARGHRMLYENPLIDAEIERWWRTVVVSQWPAPWREFGEALYRFERLCRPVYAGGATSAGDG-----W-----HERDGE 560 Kibdelosporangi...
WP_010314446 491 SLAPALEYCFATKEFAELVEQWWISTVEPAAPVQWRWALQEVVRYDLDTRPLPEPVRRGLHDA---------ELISVDGE 561 Saccharopolyspo...
PPK65224     494 SLAPALAYCFSTEEFADLVDGWWRAAVGPATPREWREALREVLRYDLDTRPLPDPGPRALPDA---------RLSSVDGE 564 Actinokineospor...
SFD85079     489 SLAPALEYCFTEPEFESLVVDWWHDRLSHAVPVEWREAVGEVLRFDLDTRPLPDAEKRGFPEA---------EPVAEGAL 559 Actinopolyspora...
WP_014748295 488 TLAPALEFCCTDRRFDALIEDWRRQVLDHRLPPRWRRSMAELIRFELETRPLVDPAHRNFP-----------EVFEDEAG 556 Tistrella mobilis

WP_051624190 578 NYYMLQDVK-FEYDIPELEKEINFGEi-PEKIEKNIYTTTLYYKVGFSDYI--ANHEFYNQFVGMFE 640 Clostridium akagii
WP_026189966 566 PSYQSTPVT-FEFDVPAALANWPQTC--TSGPKQEPVSYVFRARPGYYKNL--DNHESAATYLATSH 627 Salinispora mooreana
WP_122195388 563 AGYASDPVE-FSYDVAEILGQWEKAQ--LTAPSTAATTYVFEAQSGFYEHM--DNHETAAHYMGTPR 624 Actinomadura harenae
WP_019057203 559 DTYVSSGLVsFPFDVTAAYDRLATGD--LTAPEPLDTAYEFRSRRGFYHHL--DNHETGAHYFGTAT 621 Streptomyces prunicolor
BAI23333     561 DTYAGQGLAtFPFDVIAAYERLAAGV--LTPPEPASTAYEFRSRSGFYLHL--DNHETGAHYFATAT 623 Streptomyces griseus
WP_042183769 561 --YVSDPVV-FPYAMQDVLDAL------PNPPVQQETAYRFTAPHGFYDHL--DNHETGAHYFATAT 616 Kibdelosporangium sp. MJ126-NF4
WP_010314446 562 DHWSVERT--YRLDVPALARSAREHGe-VPEPIPDDHHVRLLYKHGFAELVrsTNHEETAHFVARPD 625 Saccharopolyspora spinosa
PPK65224     565 AHWTVART--YAFDVPALARAARARRh-LDPPAAGPHHVHLRFKHGFADLVhsTNHEETAHYIGRAE 628 Actinokineospora auranticolor
SFD85079     560 RYWAVDRE--YSHDVPELVHTAKASGelPGPPERVHTPVRLLFREGFAELArsTNHEETAHYVARVE 624 Actinopolyspora alba
WP_014748295 557 DIPHWQVIRdYDIDPPALIDRARTADrlTEPPGVGRHRFRFRFRQGFGASVrsTNHEVTAHYLARVE 623 Tistrella mobilis
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