Expression: According to AceView, this gene is moderately expressed, only 37.0% of the average gene in this release. The sequence of this gene is defined by 49 GenBank accessions from 42 cDNA clones, some from stomach (seen 12 times), liver (9), liver and spleen (6), pooled germ cell tumors (4), heart (once), ovary (once). Alternative mRNA variants and regulation: The gene contains 9 distinct gt-ag introns. Transcription produces 4 alternatively spliced mRNAs. There are 2 probable alternative promotors and 3 non overlapping alternative last exons (see the diagram). The mRNAs appear to differ by truncation of the 3' end, presence or absence of 2 cassette exons, overlapping exons with different boundaries.
Efficacy of translation may be reduced by the presence of a shorter translated product (uORF) initiating at an AUG upstream of the main open reading frame (in variant aAug10, bAug10, cAug10). Function: There is one article specifically referring to this gene in PubMed. Functionally, the gene has been tested for association to a disease (Ovarian Neoplasms). Proteins are expected to localize in various compartments (integral to membrane, plasma membrane, endoplasmic reticulum membrane, extracellular space). Protein coding potential: The 4 spliced mRNAs putatively encode good proteins, altogether 4 different isoforms (3 complete, 1 COOH complete), some containing domains Pfam, Major Facilitator Superfamily [Pfam], some transmembrane domains [Psort2]; 1 of the 3 complete proteins appears to be secreted.
Alternative mRNAs are shown aligned from 5' to 3' on a virtual genome where introns have been shrunk to a minimal length. Exon size is proportional to length, intron height reflects the number of cDNAs supporting each intron, the small numbers show the support of the introns in deep sequencing (with details in mouse-over) . Introns of the same color are identical, of different colors are different. 'Good proteins' are pink, partial or not-good proteins are yellow, uORFs are green. 5' cap or3' poly A flags show completeness of the transcript. Read more...
Mouse over the ending of each transcript gives tissues from which the supporting cDNAs were extracted. Details on tissue of origin for each intron and exon is available from the intron and exons table.
Click on any transcript to open the specific mRNA page, to see the exact cDNA clone support and eventual SNPs and to get details on tissues, sequences, mRNA and protein annotations. Proteins supported by a single continuous cDNA sequence lead to underlining the name/ending of the variant. Names not underlined result from cDNA concatenation in the coding region and should be experimentally checked.
Introns are depicted by broken lines; the height of the top of each intron reflects the relative number of clones supporting this intron. ]^[ A pink broken line denotes an intron with standard boundaries (gt-ag or gc-ag) that is exactly supported (i.e. a cDNA sequence exactly matches the genome over 16 bp, 8 on both sides of the intron). ] ^ ] A blue broken line denotes non-standard introns, exactly supported, but with non-standard at-ac or any other boundaries. ]-[ Pink and ] - ] blue straight lines represent 'fuzzy' introns of the standard and non-standard types respectively, those introns do not follow the 16 bp rule. Black straight lines ]-[denote gaps in the alignments.
Exons: Wide filled pink areas represent putative protein coding regions, narrow empty pink boxes represent the 5'UTR (on the left) and 3' UTR (on the right). Flags identify validated endings: cap site on the 5' side, polyadenylation site on the 3' side. Filled flags correspond to frequent events while empty flags have lesser supporting cDNAs (yet all are validated); at the 3' side, black flags are associated to the main AATAAA signal, blue flags to any single letter variant of the main . More explanations are given in the gene help file