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Searching 2D gels for dehydrogenases of E. coli.

  Sample User Question
Analysis/Comments
Flow Chart
Step By Step Guide
Additional Notes
 

Sample User Question back to top

Searching 2D gels for dehydrogenases of E. coli.
  • What kind of E. coli dehydrogenases have been identified in 2D gel electrophoresis?
  • Where does Isocitrate dehydrogenase map on a 2D gel?
  • Can you determine a region where the protein might map on a 2D gel of human liver?

Analysis/Comments back to top

Flow Chart back to top

  1. SWISS-2DPAGE - Two-dimensional polyacrylamide gel electrophoresis database.

  2. Sequence Retrieval System (SRS) - Network Browser for Databases in Molecular Biology

Step By Step Guide back to top

SWISS-2D PAGE

  • Select 'Access to SWISS-2DPAGE': SRS.
SRS

  • Start a search session (click Start), select a database (SWISS2D), click Continue.
  • On the Query Form page, search for escherichia coli[Organism] and dehydrogenase[Description], click Do Query.
Results:
  • 19 entries. View the complete entries by selecting complete entries (in the pull-down menu of 'Names only') and click view.
  • Scroll down to Isocitrate dehydrogenase. Select this entry. If you knew that the abbreviation was IDH, you could have selected the record from the 'Names only' (previous) page.
  • Scroll down the IDH_ECOLI page to '2D PAGE maps for identified proteins' and click a SPOT ID next to one of the E. coli gels (EPs performed at different pHs).
  • For the protein in human liver scroll further down to '2D PAGE maps for unidentified proteins' and click the gel performed from Human Liver to view the area of IDH prediction.

Additional Notes back to top



Protein/User Question 5 Return to Question Slide Revised 07/24/2003