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Genomes and Maps module of the MLA course on Introduction to Molecular Biology Information Resources
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Radiation Hybrid Map

  • similar in ways to a genetic linkage map because the distance of two markers from each other is related to the frequency with which they break apart. The higher the frequency, the greater the distance.

  • However, radiation hybrid maps are made by inducing chromosomal breakage with x-rays (rather than natural chromosomal breakage during meiosis), and have a higher resolution than genetic linkage maps.

  • use varying doses of radiation to break chromosome into pieces

    • GB4 RH panel:  3,000 Rads
      breaks DNA into approximately 10 megabase pieces
      average resolution of the map is 1 megabase
      scale of the GB4 panel is cR3000
    • G3 panel:  10,000 Rads
      breaks DNA into approximately 3 megabase pieces
      average resolution of the map is 500 kb
      scale of the G3 panel is cR10000

Genomes and Maps
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Revised 11/07/2007