BLAST: User Question and Answer
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Problem Summary:

Compare a gene sequence from normal versus diseased patients

  Sample User Question
Flow Chart
Additional Notes

Sample User Question back to top

How does the sequence obtained from patients with sleep disturbances differ from the normal sequence?



Analysis/Comments back to top

By determining which amino acids are different in normal versus diseased patients, the researchers can contribute to the understanding of circadian biology. The BLAST 2 Sequences Program (Pairwise BLAST) permits a comparison of two sequences to each other (not to the database).

Flow Chart back to top

  1. BLAST 2 Sequences, Sequence 1 - Enter patient sequence into first query box.

  2. BLAST 2 Sequences, Sequence 2 - Enter normal sequence into second query box.

  3. Choose blastp (Program) - Use pulldown menu to choose protein blast (blastp) because amino acids are being compared.

  4. Click Align

Additional Notes back to top

The results page shows a difference in one amino acid between the two sequences.

BLAST User Question Return to Slides Revised 11/05/2007
Return to Circadian Rhythms Umbrella Page