Abstract
The enzymes of 2-oxo acid decarboxylation and 2-oxo acid synthesis (EC 1.2.7.1 and EC 1.2.7.2) were isolated and partially purified from cell-free extracts of rumen micro-organisms. The lyase was active with pyruvate, 3-hydroxypyruvate and 2-oxobutyrate. The synthase was active with acetate, 2-oxoglutarate or succinate. Pyruvate synthase was separated from pyruvate lyase by Sephadex G-200 gel filtration. With Sephadex filtration, approximate mol.wts. of 310000 and 210000 were determined for pyruvate lyase and pyruvate synthase respectively.
MeSH terms
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Acetoin
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Aldehyde Oxidoreductases / isolation & purification*
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Animals
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Arsenic / pharmacology
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Butyrates
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Cadmium / pharmacology
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Cell-Free System
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Coenzyme A
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Dithiothreitol / pharmacology
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Electrophoresis, Disc
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Ferredoxins
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Flavin-Adenine Dinucleotide
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Glutathione / pharmacology
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Ketoglutaric Acids
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Lyases / isolation & purification
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Propionates
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Pyruvates
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Rumen / enzymology
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Rumen / microbiology*
Substances
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Butyrates
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Ferredoxins
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Ketoglutaric Acids
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Propionates
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Pyruvates
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Cadmium
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Flavin-Adenine Dinucleotide
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Acetoin
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Aldehyde Oxidoreductases
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Lyases
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Glutathione
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Arsenic
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Coenzyme A
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Dithiothreitol