A single cytochrome P-450 system is involved in degradation of the herbicides EPTC (S-ethyl dipropylthiocarbamate) and atrazine by Rhodococcus sp. strain NI86/21

Appl Environ Microbiol. 1995 May;61(5):2056-60. doi: 10.1128/aem.61.5.2056-2060.1995.

Abstract

During atrazine degradation by Rhodococcus sp. strain N186/21, N-dealkylated metabolites and an hydroxyisopropyl derivative are produced. The cytochrome P-450 system that is involved in degradation of thiocarbamate herbicides by strain N186/21 (I. Nagy, G. Schoofs, F. Compernolle, P. Proost, J. Vanderleyden, and R. De Mot, J. Bacteriol. 177:676-687, 1995) is also required for atrazine degradation. Atrazine-degrading activity was conferred on the atrazine-negative strains, mutant FAJ2027 of Rhodococcus sp. strain N186/21 and Rhodococcus erythropolis SQ1, upon transformation with the genes encoding the cytochrome P-450 system.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkylation
  • Atrazine / metabolism*
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism*
  • Biodegradation, Environmental
  • Cytochrome P-450 Enzyme System / genetics
  • Cytochrome P-450 Enzyme System / metabolism*
  • Enzyme Induction
  • Herbicides / metabolism*
  • Rhodococcus / enzymology*
  • Rhodococcus / genetics
  • Rhodococcus / metabolism
  • Soil Pollutants / metabolism*
  • Thiocarbamates / metabolism*

Substances

  • Bacterial Proteins
  • Herbicides
  • Soil Pollutants
  • Thiocarbamates
  • Cytochrome P-450 Enzyme System
  • Atrazine
  • EPTC