On the origins of cyanuric acid hydrolase: purification, substrates, and prevalence of AtzD from Pseudomonas sp. strain ADP

Isaac Fruchey; Nir Shapir; Michael J Sadowsky; Lawrence P Wackett

doi:10.1128/AEM.69.6.3653-3657.2003

On the origins of cyanuric acid hydrolase: purification, substrates, and prevalence of AtzD from Pseudomonas sp. strain ADP

Appl Environ Microbiol. 2003 Jun;69(6):3653-7. doi: 10.1128/AEM.69.6.3653-3657.2003.

Authors

Isaac Fruchey¹, Nir Shapir, Michael J Sadowsky, Lawrence P Wackett

Affiliation

¹ Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, St. Paul, Minnesota 55108, USA.

Abstract

Cyanuric acid hydrolase (AtzD) from Pseudomonas sp. strain ADP was purified to homogeneity. Of 22 cyclic amides and triazine compounds tested, only cyanuric acid and N-methylisocyanuric acid were substrates. Other cyclic amidases were found not to hydrolyze cyanuric acid. Ten bacteria that use cyanuric acid as a sole nitrogen source for growth were found to contain either atzD or trzD, but not both genes.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Amidohydrolases / genetics
Amidohydrolases / isolation & purification*
Amidohydrolases / metabolism*
Bacterial Proteins / genetics
Bacterial Proteins / isolation & purification
Bacterial Proteins / metabolism
Evolution, Molecular*
Gram-Negative Bacteria / enzymology
Gram-Negative Bacteria / genetics
Herbicides / metabolism
Pseudomonas / enzymology*
Pseudomonas / genetics*
Substrate Specificity
Triazines / chemistry
Triazines / metabolism

Substances

Bacterial Proteins
Herbicides
Triazines
Amidohydrolases
cyanuric acid amidohydrolase
cyanuric acid