Abstract
Five genes involved in the two initial steps of the tetralin biodegradation pathway of Sphingomonas macrogolitabida strain TFA have been characterized. ThnA1A2 and ThnA3A4, components of the ring-hydroxylating dioxygenase, were encoded in divergently transcribed operons. ThnA1, ThnA2, and ThnA3 were essential for tetralin ring-hydroxylating dioxygenase activity. ThnB was identified as a dehydrogenase required for tetralin biodegradation.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Bacterial Proteins / genetics
-
Bacterial Proteins / metabolism
-
Biodegradation, Environmental
-
Culture Media
-
Molecular Sequence Data
-
Operon
-
Oxidoreductases / genetics*
-
Oxidoreductases / metabolism
-
Oxygenases / genetics*
-
Oxygenases / metabolism
-
Solvents / metabolism*
-
Sphingomonas / enzymology*
-
Sphingomonas / genetics
-
Sphingomonas / growth & development
-
Tetrahydronaphthalenes / metabolism*
Substances
-
Bacterial Proteins
-
Culture Media
-
Solvents
-
Tetrahydronaphthalenes
-
Oxidoreductases
-
Oxygenases
-
tetralin