Abstract
The Moorella thermoacetica aromatic O-demethylase was characterized as an inducible three-component system with similarity to the methanogenic methanol, methylamine, and methanethiol methyltransferases and to the O-demethylase system from Acetobacterium dehalogenans. MtvB catalyzes methyl transfer from a phenylmethylether to the cobalt center of MtvC, a corrinoid protein. MtvA catalyzes transmethylation from MtvC to tetrahydrofolate, forming methyltetrahydrofolate. Cobalamin can substitute for MtvC.
MeSH terms
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Amino Acid Sequence
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Anaerobiosis
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Bacterial Proteins / chemistry
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism
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Clostridium / enzymology*
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Clostridium / growth & development
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Dicamba / metabolism
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Gram-Positive Rods / enzymology
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Gram-Positive Rods / growth & development
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Hydroxocobalamin / metabolism
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Kinetics
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Methylation
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Methyltransferases / metabolism
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Molecular Sequence Data
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Oxidoreductases, O-Demethylating / chemistry
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Oxidoreductases, O-Demethylating / genetics*
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Oxidoreductases, O-Demethylating / isolation & purification*
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Oxidoreductases, O-Demethylating / metabolism
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Tetrahydrofolates / metabolism
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Vanillic Acid / metabolism*
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Vitamin B 12 / analogs & derivatives*
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Vitamin B 12 / metabolism
Substances
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Bacterial Proteins
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Tetrahydrofolates
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5,6,7,8-tetrahydrofolic acid
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mecobalamin
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Oxidoreductases, O-Demethylating
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vanillate O-demethylase
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Methyltransferases
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Vanillic Acid
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Vitamin B 12
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Hydroxocobalamin
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Dicamba