The two forms of myosin were stained with specific antibodies, each coupled to a different fluorescent dye, and examined in a fluorescence microscope. Myosin-II ( orange) shows the highest accumulation in the posterior cortex, whereas myosin-I ( green) is mainly restricted to the leading edge of lamellipodia at the front of the cell. Some myosin is also seen in phagocytic vesicles in the cytoplasm. (Courtesy of Yoshio Fukui.)