PCR Template
(A refseq record is preferred) [?] Clear

From To
[?] Clear  
Primer Parameters
[?] Clear
[?] Clear
[?]

Exon/intron selection
[?]
[?]

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[?]

Note: Parameter values that differ from the default are highlighted in yellow
Primer Pair Specificity Checking Parameters
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Clear

Or, upload file:

Enter an organism name, taxonomy id or select from the suggestion list as you type. [?]

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Primer must have at least total mismatches to unintended targets, including
at least mismatches within the last bps at the 3' end. [?]
Ignore targets that have or more mismatches to the primer. [?]
Note the parameter change [?]
[?]
Get Primer
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Advanced parameters Note: Parameter values that differ from the default are highlighted in yellow