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SRX978714: GSM1650088: L8 replicate 3; Oryza sativa Japonica Group; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 35.2M spots, 3.6G bases, 2.3Gb downloads

Submitted by: Gene Expression Omnibus (GEO)
Study: Host transcriptional reprogramming across diverse strains of the rice bacterial leaf streak pathogen Xanthomonas oryzae pv.oryzicola
show Abstracthide Abstract
We performed RNA-Seq of leaves of Oryza sativa L. ssp. japonica cv. Nipponbare 48 hours after inoculation with 10 geographically diverse strains of Xanthomonas oryzae pv. oryzicola, the causal agent of bacterial leaf streak. Results provide insight into the molecular basis of bacterial leaf streak, particularly the role of transcription activator-like effectors in the disease. Overall design: Examination of mRNA levels in Oryza sativa L. ssp. japonica cv. Nipponbare leaves at 48 hours after inoculation with 10 strains of Xanthomonas oryzae pv.oryzicola with three biological replicates for each compared to three replicates of mock inoculated O sativa as the control
Sample: L8 replicate 3
SAMN03460271 • SRS895410 • All experiments • All runs
Library:
Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: RNA was extracted using a modified hot Trizol protocol (Huang et al., 2005) followed by treatment with RNase-free DNase I (Life Technologies, Carlsbad, CA), then purified using the RNeasy MinElute Cleanup kit (Qiagen, Valencia, CA). These libraries were prepped with the Illumina TruSeq RNA Sample Prep v2 kit
Experiment attributes:
GEO Accession: GSM1650088
Links:
External link:
Runs: 1 run, 35.2M spots, 3.6G bases, 2.3Gb
Run# of Spots# of BasesSizePublished
SRR195279235,221,8923.6G2.3Gb2015-05-01

ID:
1423755

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