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SRX885611: 4deg_incubation_metatranscriptome
1 ILLUMINA (Illumina HiSeq 2000) run: 30.3M spots, 6.1G bases, 4.1Gb downloads

Design: Sample from SRS351311 were incubated anoxically at 4 degrees for 1 month. Phenol/chloroform extraction, RNA linearly amplified using the Message amp II bacteria kit, ampRNA was reversely transcribed with random hexamer primed cDNA synthesis, sequenced with Illumina HighSeq2000.
Submitted by: UNIVERSITY OF TROMSØ
Study: Peat Soil Metagenome
show Abstracthide Abstract
We have in this study applied, for the first time, a combined metagenomic and metatranscriptomic approach on high-Arctic peat soils to obtain on-site information about the genomic potential for SOC transformation and to identify the active microorganisms driving these processes. We compared the Arctic peat metagenomes with metagenomes from temperate soils and proposed a model of SOC degradation in Arctic peat, including anaerobic respiratory and fermentative pathways, and methanogenic and methanotrophic microorganisms. High Arctic peat soil from Svalbard.
Sample: MIMS Environmental/Metagenome sample from Microbial_community
SAMN01090429 • SRS351311 • All experiments • All runs
Organism: soil metagenome
Library:
Name: 4deg_mt
Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Source: METATRANSCRIPTOMIC
Selection: RANDOM
Layout: PAIRED
Spot descriptor:
forward102  reverse

Runs: 1 run, 30.3M spots, 6.1G bases, 4.1Gb
Run# of Spots# of BasesSizePublished
SRR195884230,295,1076.1G4.1Gb2015-04-13

ID:
1272470

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