show Abstracthide AbstractComparison of NextSeq 500, HiSeq, GAIIx and MiSeq Platforms: resequencing of PhiX Phage genome. At Genotypic Technology's NGS lab, we compared the data from NextSeq, HiSeq, GAIIx and MiSeq Illumina platforms using the data output from the control libraries. HiSeq (100 base pair paired end reads), NextSeq 500 (150 base pair paired end reads), GAIIx (36 base pair reads) and MiSeq (300 base pair Paired end reads) resulted in comparable alignment to the reference genome and better assembly with increase in lengths, the detailed analysis will be published else where. The data provided is 134.17M reads of 150 base paired end reads from NextSeq 500 sequencer. This data can be used for QC of Nextseq data as well as comparison of data from GAIIx, HiSeq and MiSeq platforms which use the same PhiX phage genome as a control.