RNA-Seq (Total RNA) Fetal Muscle, Leg - SRA

SRX252242: RNA-Seq (Total RNA) Fetal Muscle, Leg
1 ILLUMINA (Illumina HiSeq 2000) run: 51.9M spots, 1.9G bases, 1.1Gb downloads

Design: Illumina TruSeq RNA PART# 15008136 REVISION A

Submitted by: Gene Expression Omnibus (GEO)

Study: University of Washington Human Reference Epigenome Mapping Project

• SRP001371 • All experiments • All runs

show Abstracthide Abstract

Study of chromatin accessibility

Sample: Generic sample from Homo sapiens

SAMN00739211 • SRS267039 • All experiments • All runs

Organism: Homo sapiens

Library:

Name: lib-RNA.RS19855

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Spot descriptor:

1 forward

Experiment attributes: (show all 17 attributes...) (hide...)

EXPERIMENT_TYPE: mRNA-Seq

EXTRACTION_PROTOCOL: Ambion mirVana miRNA isolation kit

EXTRACTION_PROTOCOL_MRNA_ENRICHMENT: Part of Library Prep, Truseq RNA-Seq Sample Prep K (show full text...)(hide...)

Part of Library Prep, Truseq RNA-Seq Sample Prep Kit

RNA_PREPARATION_INITIAL_RNA_QNTY: 1ug

RNA_PREPARATION_REVERSE_TRANSCRIPTION_PRIMER_SEQUENCE: Random Primers(hexamers)

RNA_PREPARATION_REVERSE_TRANSCRIPTION_PROTOCOL: TruSeq RNA

LIBRARY_GENERATION_PCR_TEMPLATE: cDNA

LIBRARY_FRAGMENTATION: Part of Library Prep, Truseq RNA-Seq Sample Prep K (show full text...)(hide...)

Part of Library Prep, Truseq RNA-Seq Sample Prep Kit

LIBRARY_FRAGMENT_SIZE_RANGE: 120-200bp

LIBRARY_GENERATION_PCR_POLYMERASE_TYPE: Proprietary

LIBRARY_GENERATION_PCR_THERMOCYCLING_PROGRAM: 98C 30 sec, 15 cycles of 98C 10 sec, 65C 30 sec, 7 (show full text...)(hide...)

98C 30 sec, 15 cycles of 98C 10 sec, 65C 30 sec, 72C 30 sec, then 72C 5 min, 4C hold

LIBRARY_GENERATION_PCR_NUMBER_CYCLES: 15

LIBRARY_GENERATION_PCR_F_PRIMER_SEQUENCE: Proprietary

LIBRARY_GENERATION_PCR_R_PRIMER_SEQUENCE: Proprietary

LIBRARY_GENERATION_PCR_PRIMER_CONC: Proprietary

LIBRARY_GENERATION_PCR_PRODUCT_ISOLATION_PROTOCOL: Part of Library Prep, Truseq RNA-Seq Sample Prep K (show full text...)(hide...)

Part of Library Prep, Truseq RNA-Seq Sample Prep Kit

GEO Accession: GSM1101664

Pipeline: show...hide...

Name	Step	Prev Step	Program	Version
BASE_CALLS	1	NIL	Illumina RTA	1.12.4.2
QUALITY_SCORES	2	1	Illumina RTA	1.12.4.2

Links:

External link: EDACC Genboree Page

External link: GEO Web Link

Runs: 1 run, 51.9M spots, 1.9G bases, 1.1Gb

Run	# of Spots	# of Bases	Size	Published
SRR786788	51,941,901	1.9G	1.1Gb	2013-03-19

ID:: 352796

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