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SRX202921: Gernera ray floret transcripome
1 ILLUMINA (Illumina HiSeq 2000) run: 32.8M spots, 5.9G bases, 3.9Gb downloads

Accession: SRX202921
Experiment design: The cDNA library preparation and sequencing reactions were conducted at the Beijing Genomics Institute (BGI) genomic centre, Shenzhen, China. Magnetic beads with Oligo(dT) were used to isolate the poly(A)+ mRNA after all of the total high quality 20 µg RNA samples were mixed. Fragmentation buffer was added in the presence of divalent cations at 94?for 5 min to interrupt the mRNA into short fragments of approximately 200 bp. These short fragments were used as templates, and random hexamer-primer was used to synthesize the first-strand cDNA. The second-strand cDNA was synthesized using buffer, dNTPs, RNaseH and DNA polymerase I. The short fragments were purified with a QiaQuick PCR extraction kit and resolved with EB buffer to end reparation and add poly(A)+. The suitable fragments (approximately 375 bp) with sequencing adaptors were selected as templates for the PCR amplification based on the agarose gel electrophoresis results. The samples were clustered in flow cells to construct the cDNA library and loaded onto an Illumina HiSeq™ 2000.
Submitted by: southchina normal university
Study summary: SRP017069 • Gerbera hybrid cultivar strain:Shenzhen No.5 Transcriptome or Gene expression • PRJNA179026 • All experiments  • Run Selector (more...)(less...)
Abstract: In order to enrich the study of Asteraceae flower development, the transcriptome of gerbera ray florets using RNA-seq was constructed for understanding the molecular mechanisms. The ray florets in five sample pools from early p1 and P1, P2 to P5 to constitute our cDNA library for the transcriptome in the gerbera flower growth. All fresh samples collected were snap-frozen immediately in nitrogen and stored at -80? to extract the total RNA.Total RNA was extracted using the TRIZol reagent and an isolation system according to the manufacturer’s protocol and treated with DNase. To avoid losing low expression transcripts during early envelopment, a pooled RNA sample with early P1 and P1, P2, P3, P4 and P5 was mixed in a ratio of 2:1.25:1:1:1 after measuring the integrity.
Center Project: Gerbera hybrid cultivar
Sample: SAMN01801655 • Gerebra hybrida ray floret RNA samples (more...)(less...)
Organism: Gerbera
Library:   (more...)(less...)
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: size fractionation
Layout: PAIRED, Nominal length: 200
Platform: Illumina  (more...)(less...)
Instrument model: Illumina HiSeq 2000
Spot descriptor:
forward91  reverse

Total: 1 run, 32.8M spots, 5.9G bases, 3.9Gb 
#Run# of Spots# of BasesSizePublished
1. SRR61139732,780,7645.9G3.9Gb2013-02-01

ID:
270275

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