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SRX185622: Dehydration Experiment 2
1 ILLUMINA (Illumina Genome Analyzer II) run: 5.2M spots, 792.1M bases, 400.4Mb downloads

Accession: SRX185622
Experiment design: Total RNA was extracted from larvae using Trizol reagent (Life Technologies, Grand Island, NY) according to the manufacturer’s protocol. RNA quantity and purity was assessed on a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA), and integrity was measured on an Agilent Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA). To generate RNA-seq libraries, we used the Illumina TruSeq RNA Sample Preparation kit (Illumina, San Diego, CA) according to the manufacturer’s protocol. In short, mRNA was purified from 2 µg total RNA from each sample, fragmented, and converted to double-stranded cDNA. Sequencing barcodes were ligated to the cDNA fragments, and the resulting fragments were amplified using PCR. Libraries were validated on an Agilent Bioanalyzer 2100 to ensure the libraries had the expected fragment size of ~300 bp.
Submitted by: The Ohio State University
Study summary: SRP015638 • Belgica antarcticaTranscriptome or Gene expression • PRJNA174315 • All experiments  • Run Selector (more...)(less...)
Abstract: The Antarctic midge, Belgica antarctica, is the world’s southernmost free-living insect and the only true insect endemic to Antarctica. In this environment, water is frozen and hence unavailable for much of the year, thus exposing larvae to a constant risk of desiccation. In this study, we used RNA-seq to profile gene expression both in response to desiccation at a constant temperature and in response to cryoprotective dehydration, an overwintering strategy in which gradual decreases in temperature cause water to be lost to surrounding ice, allowing the larvae to remain unfrozen at subzero temperatures. Larve of B. antarctica were collected on offshore islands near Palmer Station (64°46’S, 64°04’W) in January, 2010.
Center Project: Belgica antarctica
Sample: SAMN01163214 • Dehydration Sample 2 (more...)(less...)
Library:   (more...)(less...)
Strategy: RNA-Seq
Selection: PCR
Layout: PAIRED, Nominal length: 300
Platform: Illumina  (more...)(less...)
Instrument model: Illumina Genome Analyzer II
Spot descriptor:
forward77  reverse

Total: 1 run, 5.2M spots, 792.1M bases, 400.4Mb 
#Run# of Spots# of BasesSize
1. SRR5671675,211,200792.1M400.4Mb


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