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SRX181880: Mud loach skin transcriptome sequencing
3 ILLUMINA (Illumina Genome Analyzer IIx) runs: 55.5M spots, 4G bases, 2.1Gb downloads

Accession: SRX181880
Experiment design: cDNA library construction was performed at SinoGenoMax Co., Ltd, Beijing, China (http://www.sinogenomax.com/). Before library construction, the integrity of RNA sample was confirmed using Agilent 2100 Bioanalyzer (with a RNA integrity number of 9). 4 µg of Total RNA was used for isolation of polyA RNA with Sera-mag Magnetic Oligo (dT) Beads (Illumina). The purified mRNA was fragmented into small pieces (100-400 bp) using divalent cations at 94°C for 5 minutes. Double-stranded cDNA was synthesized using the SuperScript Double-Stranded cDNA Synthesis kit (Invitrogen, Camarillo, CA) with random hexamer primers (Illumina). The synthesized cDNA was subjected to end-repair, phosphorylation, 3’ adenylation and adapter ligation in sequential. After these steps, cDNA fragments at the length of 300 ± 50 bp were selected and purified by gel electrophoresis. Finally, the purified cDNA template was enriched by PCR amplification and the quality of the library was validated in a LightCycler480 (Roche Diagnostics) with an Illumina PhiX174 Control.
Submitted by: Institute of Hydrobiology, Chinese Academy of Scie
Study summary: SRP017295 • Misgurnus anguillicaudatusTranscriptome or Gene expression • PRJNA173219 • All experiments  • Run Selector (more...)(less...)
Abstract: To characterize the skin transcriptome of mud loach (Misgurnus anguillicaudatus), especially for discovery of genes associated with immue and mucus synthesis.
Center Project: Misgurnus anguillicaudatus
Sample: SAMN01121008 • Mud loach skin (more...)(less...)
Library:   (more...)(less...)
Strategy: RNA-Seq
Selection: PCR
Layout: PAIRED, Nominal length: 300
Platform: Illumina  (more...)(less...)
Instrument model: Illumina Genome Analyzer IIx
Spot descriptor:
forward37  reverse

Total: 3 runs, 55.5M spots, 4G bases, 2.1Gb 
#Run# of Spots# of BasesSize
1. SRR55014712,742,149917.4M513.7Mb
2. SRR55014828,732,0082.1G1.1Gb
3. SRR55014914,011,7761G531.6Mb


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