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SRX1999920: PQ_3
4 ILLUMINA (NextSeq 500) runs: 28.9M spots, 7.9G bases, 3.3Gb downloads

Design: Total RNA was isolated using the Plant RNA Qiagen RNeasy kit according to the manufacturer’s instruction (Qiagen,Valencia, CA, USA). RNA quantity and quality control was performed using a NanoDrop ND-1000 spectrophotometer (NanoDrop Technologies, DE, USA) and the Agilent 2100 Bioanalyzer (Agilent Technologies, CA, USA). Library preparation was performed using the Illumina (USA) TruSeq kit and 150 bp paired-end sequencing was performed in one lane of an Illumina NextSeq 500 sequencer using the NextSeq High Output kit (300 cycles, Illumina, USA) according to the manufacturer’s instructions.
Submitted by: KU Leuven
Study: RNA-Seq analysis of biotic and abiotic stress response in Arabidopsis thaliana
show Abstracthide Abstract
An important feature of plant stress response comprises production of several bioactive peptides. Many eukaryotic RNAs have been considered non-coding as they contain only short open reading frames (sORFs). However, there has been increasing evidence for the translation of these sORFs into bioactive peptides. Yet only a few small peptides are annotated in the model organism Arabidopsis thaliana. Our research focuses on the discovery of novel peptides to enrich the existing inventory of bioactive peptides in Arabidopsis thaliana.
Sample: treated with PQ
SAMN05511245 • SRS1600711 • All experiments • All runs
Library:
Instrument: NextSeq 500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: PolyA
Layout: PAIRED
Spot descriptor:
forward151  reverse

Runs: 4 runs, 28.9M spots, 7.9G bases, 3.3Gb
Run# of Spots# of BasesSizePublished
SRR39997047,164,6152G855.3Mb2016-12-24
SRR39997057,091,1781.9G846.2Mb2016-12-24
SRR39997067,394,0282G874.4Mb2016-12-24
SRR39997077,219,3812G852.4Mb2016-12-24

ID:
2875836

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