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SRX831217: GSM1576719: SMARTseq2 - 0%; Drosophila melanogaster; Drosophila virilis; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 2.4M spots, 122M bases, 83Mb downloads

Submitted by: Gene Expression Omnibus (GEO)
Study: Low-cost, low-input RNA-seq protocols perform nearly as well as high-input protocols
PRJNA271674 • SRP051726 • All experiments • All runs
show Abstracthide Abstract
We sequenced mRNA according to several library prep protocols with known mixtures of two species of Drosophila in order to establish linear response in each protocol. Overall design: For each library prep protocol, mixtures with 0%, 5%, 10%, and 20% D. virilis total RNA was prepared, then libraries prepared according to instructions.
Sample: SMARTseq2 - 0%
SAMN03275753 • SRS809662 • All experiments • All runs
Organism: Drosophila melanogaster
Library:
Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: TRIzol extraction of pools of whole embryos Illumina TruSeq mRNA Unstranded Epicentre TotalScript: oligo(dT) Primed Clontech Low Volume Library Construction Kit SMARTseq v2 SMARTseq v2 - Tagmentation volume 1/2.5 SMARTseq v2 - Tagmentation volume 1/5
Experiment attributes:
GEO Accession: GSM1576719
Links:
External link:
Runs: 1 run, 2.4M spots, 122M bases, 83Mb
Run# of Spots# of BasesSizePublished
SRR17431532,439,518122M83Mb2015-01-08

ID:
1180992

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