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ERX301048: qiime_experiment_1056:631115:20
1 ILLUMINA (unspecified) run: 21,572 spots, 3.2M bases, 2.1Mb downloads

Accession: ERX301048
Experiment design: Comparison of microbial flora in ant-eating mammals
Submitted by: CCME-COLORADO
Study summary: ERP003782 • Convergence of gut microbiomes in myrmecophagous mammals • PRJEB4489 • All experiments  • Run Selector (more...)(less...)
Abstract: Mammals have diversified into many dietary niches. Specialized myrmecophagous (ant- and termite-eating) placental mammals represent a textbook example of evolutionary convergence driven by extreme diet specialization. Armadillos, anteaters, aardvarks, pangolins, and aardwolves thus provide a model system for understanding the potential role of gut microbiota in the convergent adaptation to myrmecophagy. Here, we expand upon previous mammalian gut microbiome studies by using high-throughput barcoded Illumina sequencing of the 16S rRNA gene to characterize the composition of gut microbiota in 15 species representing all placental myrmecophagous lineages and their close relatives from zoo- and field-collected samples. We confirm that both diet and phylogeny drive the evolution of mammalian gut microbiota, with cases of convergence in global composition, but also examples of phylogenetic inertia. Our results reveal specialized placental myrmecophages as a spectacular case of large-scale convergence in gut microbiome composition. Indeed, Neighbor-Net networks and beta diversity plots based on UniFrac distances show significant clustering of myrmecophagous species (anteaters, aardvarks, and aardwolves) even though they belong to phylogenetically distant lineages representing different orders. The aardwolf, which diverged from carnivorous hyenas only in the last 10 million years, experienced a convergent shift in the composition of its gut microbiome to become more similar to other myrmecophages. These results confirm diet adaptation to be a major driving factor of convergence in gut microbiome composition over evolutionary timescales. This study sets the scene for future metagenomic studies aiming at evaluating potential convergence in functional gene content in the microbiomes of specialized mammalian myrmecophages.
Attributes:
y
0
y
fecal
mimarks-survey
EMP
n
Delsuc_anteaters
Delsuc_microbi_ant
93
Comparison of microbial flora in xenarthrans
y
Mammals have diversified into many dietary niches. Specialized myrmecophagous (ant- and termite-eating) placental mammals represent a textbook example of evolutionary convergence driven by extreme diet specialization. Armadillos, anteaters, aardvarks, pangolins, and aardwolves thus provide a model system for understanding the potential role of gut microbiota in the convergent adaptation to myrmecophagy. Here, we expand upon previous mammalian gut microbiome studies by using high-throughput barcoded Illumina sequencing of the 16S rRNA gene to characterize the composition of gut microbiota in 15 species representing all placental myrmecophagous lineages and their close relatives from zoo- and field-collected samples. We confirm that both diet and phylogeny drive the evolution of mammalian gut microbiota, with cases of convergence in global composition, but also examples of phylogenetic inertia. Our results reveal specialized placental myrmecophages as a spectacular case of large-scale convergence in gut microbiome composition. Indeed, Neighbor-Net networks and beta diversity plots based on UniFrac distances show significant clustering of myrmecophagous species (anteaters, aardvarks, and aardwolves) even though they belong to phylogenetically distant lineages representing different orders. The aardwolf, which diverged from carnivorous hyenas only in the last 10 million years, experienced a convergent shift in the composition of its gut microbiome to become more similar to other myrmecophages. These results confirm diet adaptation to be a major driving factor of convergence in gut microbiome composition over evolutionary timescales. This study sets the scene for future metagenomic studies aiming at evaluating potential convergence in functional gene content in the microbiomes of specialized mammalian myrmecophages.
Convergence of gut microbiomes in myrmecophagous mammals
93
Center Project:
Sample: SAMEA2171474 • Das.nov.Flori.7 (more...)(less...)
Organism: gut metagenome
Attributes:
y
47
631115
gut metagenome
.1,g
Dasypus novemcinctus
Xenarthra
Dasypodidae
ENVO:animal-associated habitat
n
Comparison of microbial flora in ant-eating mammals
3/10/2012
Cingulata
0.0
505
ENVO:animal-associated habitat
novemcinctus
3.0
749906
Das.nov.Flori.7
749906
CCME
UBERON:feces
fecal sample from Nine-banded armadillo
fecal sample
juvenile
Insectivore
28.516667
Nine-banded armadillo
y
1056:1056:MRI007
UBERON:feces
507
GAZ:United States of America
P2_B8
-80.666667
ants, insects, soil invertebrates
0
wild
y
UBERON:feces
Dasypus
ENVO:feces
Simple
MRI007
ERC000011
Library: Das.nov.Flori.7:20  (more...)(less...)
Strategy: AMPLICON
Source: METAGENOMIC
Selection: PCR
Layout: SINGLE
Construction protocol: EMP Illumina HiSeq V4
Platform: Illumina  (more...)(less...)
Instrument model: unspecified
Spot descriptor:

Experiment attributes:
experiment_title: Comparison of microbial flora in ant-eating mammal (show full text...)(hide...)
Comparison of microbial flora in ant-eating mammals
target_subfragment: V4
sample_center: CCME
sequencing_meth: sequencing by synthesis
run_date: 10/9/12
experiment_center: CCME
pcr_primers: FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT
run_center: CCME
num_sequences: 21572
platform: Illumina
library_construction_protocol: EMP Illumina HiSeq V4
experiment_design_description: Comparison of microbial flora in ant-eating mammal (show full text...)(hide...)
Comparison of microbial flora in ant-eating mammals
row_number: 20
target_gene: 16S rRNA
study_center: CCME
Total: 1 run, 21,572 spots, 3.2M bases, 2.1Mb 
#Run# of Spots# of BasesSize
1. ERR32800921,5723.2M2.1Mb

ID:
472730

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