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ERX141791: RNA-seq on DGRP lines and F1 crosses
1 ILLUMINA (Illumina Genome Analyzer II) run: 14.8M spots, 1.2G bases, 736.5Mb downloads

Design: RNA-seq on DGRP lines and F1 crosses
Submitted by: EPFL Institute of Bioengineering
Study: RNA-seq on DGRP lines and F1 crosses
show Abstracthide Abstract
We performed mRNA sequencing of reciprocal F1 female hybrids from two crosses (362/765 and 517/765) and the parental DGRP lines (362, 517 and 765). Specifically, we aimed to identify whether transcripts predicted to be regulated by cis-eQTLs exhibit a significant allele-specific bias in gene expression. Since both alleles act in the cross in the same trans environment, differential expression in the F1 is a direct measure of cis-regulatory activity.
Sample: Drosophila melanogaster; 16_A12_pUnt1MIsZu7i
SAMEA1487535 • ERS167419 • All experiments • All runs
Name: RNA Extract 11
Instrument: Illumina Genome Analyzer II
Strategy: RNA-Seq
Selection: RANDOM
Layout: SINGLE
Construction protocol: Total RNA was extracted using the combined Trizol/RNeasy (Qiagen, www1.Qiagen.com) protocol. RNA quality was measured using RNA Labchips and Bioanalyzer from Agilent Technologies (http://www.chem.agilent.com). RNA-seq libraries were prepared using the RNA-True seq kit (Illumina).
Experiment attributes:
Experimental Factor: StrainOrLine: DGRP-362/765
Runs: 1 run, 14.8M spots, 1.2G bases, 736.5Mb
Run# of Spots# of BasesSizePublished


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