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ERX141784: RNA-seq on DGRP lines and F1 crosses
1 ILLUMINA (Illumina Genome Analyzer II) run: 15M spots, 600.8M bases, 357.4Mb downloads

Accession: ERX141784
Experiment design: RNA-seq on DGRP lines and F1 crosses
Submitted by: EPFL
Study summary: ERP001746 • RNA-seq on DGRP lines and F1 crosses • PRJEB3230 • All experiments  • Run Selector (more...)(less...)
Abstract: We performed mRNA sequencing of reciprocal F1 female hybrids from two crosses (362/765 and 517/765) and the parental DGRP lines (362, 517 and 765). Specifically, we aimed to identify whether transcripts predicted to be regulated by cis-eQTLs exhibit a significant allele-specific bias in gene expression. Since both alleles act in the cross in the same trans environment, differential expression in the F1 is a direct measure of cis-regulatory activity.
Center Project: RNA-seq on DGRP lines and F1 crosses
Sample: SAMEA1487540 • 17_A4_kstIHSBwdfJ4 (more...)(less...)
Drosophila melanogaster
3 to 5 day
Library: RNA Extract 5  (more...)(less...)
Strategy: RNA-Seq
Selection: RANDOM
Layout: SINGLE
Construction protocol: Total RNA was extracted using the combined Trizol/RNeasy (Qiagen, www1.Qiagen.com) protocol. RNA quality was measured using RNA Labchips and Bioanalyzer from Agilent Technologies (http://www.chem.agilent.com). RNA-seq libraries were prepared using the RNA-True seq kit (Illumina).
Platform: Illumina  (more...)(less...)
Instrument model: Illumina Genome Analyzer II
Spot descriptor:

Experiment attributes:
Experimental Factor: StrainOrLine: DGRP-765/362
Total: 1 run, 15M spots, 600.8M bases, 357.4Mb 
#Run# of Spots# of BasesSize
1. ERR16591415,020,680600.8M357.4Mb


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