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Fritz ZR, Schloss RS, Yarmush ML, Williams LJ. HSymM-guided engineering of the immunodominant p53 transactivation domain putative peptide antigen for improved binding to its anti-p53 monoclonal antibody. Bioorg Med Chem Lett. 2021 Nov 1;51:128341. doi: 10.1016/j.bmcl.2021.128341. Epub 2021 Aug 26. PubMed PMID: 34454062; PubMed Central PMCID: PMC8526406.
Li XP, Harijan RK, Cao B, Kahn JN, Pierce M, Tsymbal AM, Roberge JY, Augeri D, Tumer NE. Synthesis and Structural Characterization of Ricin Inhibitors Targeting Ribosome Binding Using Fragment-Based Methods and Structure-Based Design. J Med Chem. 2021 Oct 28;64(20):15334-15348. doi: 10.1021/acs.jmedchem.1c01370. Epub 2021 Oct 14. PubMed PMID: 34648707; PubMed Central PMCID: PMC10704857.
Rudolph MJ, Davis SA, Tumer NE, Li XP. Structural basis for the interaction of Shiga toxin 2a with a C-terminal peptide of ribosomal P stalk proteins. J Biol Chem. 2020 Nov 13;295(46):15588-15596. doi: 10.1074/jbc.AC120.015070. Epub 2020 Sep 2. PubMed PMID: 32878986; PubMed Central PMCID: PMC7667979.
Li XP, Harijan RK, Kahn JN, Schramm VL, Tumer NE. Small Molecule Inhibitors Targeting the Interaction of Ricin Toxin A Subunit with Ribosomes. ACS Infect Dis. 2020 Jul 10;6(7):1894-1905. doi: 10.1021/acsinfecdis.0c00127. Epub 2020 Jun 8. PubMed PMID: 32428396; PubMed Central PMCID: PMC7678679.
Pierce M, Vengsarkar D, McLaughlin JE, Kahn JN, Tumer NE. Ribosome depurination by ricin leads to inhibition of endoplasmic reticulum stress-induced HAC1 mRNA splicing on the ribosome. J Biol Chem. 2019 Nov 22;294(47):17848-17862. doi: 10.1074/jbc.RA119.009128. Epub 2019 Oct 17. PubMed PMID: 31624149; PubMed Central PMCID: PMC6879324.
Zhou Y, Li XP, Kahn JN, McLaughlin JE, Tumer NE. Leucine 232 and hydrophobic residues at the ribosomal P stalk binding site are critical for biological activity of ricin. Biosci Rep. 2019 Oct 30;39(10). doi: 10.1042/BSR20192022. PubMed PMID: 31548364; PubMed Central PMCID: PMC6822507.
Li XP, Kahn JN, Tumer NE. Peptide Mimics of the Ribosomal P Stalk Inhibit the Activity of Ricin A Chain by Preventing Ribosome Binding. Toxins (Basel). 2018 Sep 13;10(9). doi: 10.3390/toxins10090371. PubMed PMID: 30217009; PubMed Central PMCID: PMC6162817.
Zhou Y, Li XP, Kahn JN, Tumer NE. Functional Assays for Measuring the Catalytic Activity of Ribosome Inactivating Proteins. Toxins (Basel). 2018 Jun 14;10(6). doi: 10.3390/toxins10060240. Review. PubMed PMID: 29899209; PubMed Central PMCID: PMC6024586.
Grela P, Li XP, Horbowicz P, Dźwierzyńska M, Tchórzewski M, Tumer NE. Human ribosomal P1-P2 heterodimer represents an optimal docking site for ricin A chain with a prominent role for P1 C-terminus. Sci Rep. 2017 Jul 17;7(1):5608. doi: 10.1038/s41598-017-05675-5. PubMed PMID: 28717148; PubMed Central PMCID: PMC5514047.
Li XP, Tumer NE. Differences in Ribosome Binding and Sarcin/Ricin Loop Depurination by Shiga and Ricin Holotoxins. Toxins (Basel). 2017 Apr 11;9(4). doi: 10.3390/toxins9040133. PubMed PMID: 28398250; PubMed Central PMCID: PMC5408207.
Zhou Y, Li XP, Chen BY, Tumer NE. Ricin uses arginine 235 as an anchor residue to bind to P-proteins of the ribosomal stalk. Sci Rep. 2017 Feb 23;7:42912. doi: 10.1038/srep42912. PubMed PMID: 28230053; PubMed Central PMCID: PMC5322317.
Basu D, Kahn JN, Li XP, Tumer NE. Conserved Arginines at the P-Protein Stalk Binding Site and the Active Site Are Critical for Ribosome Interactions of Shiga Toxins but Do Not Contribute to Differences in the Affinity of the A1 Subunits for the Ribosome. Infect Immun. 2016 Dec;84(12):3290-3301. doi: 10.1128/IAI.00630-16. Print 2016 Dec. PubMed PMID: 27600507; PubMed Central PMCID: PMC5116708.
Jetzt AE, Li XP, Tumer NE, Cohick WS. Toxicity of ricin A chain is reduced in mammalian cells by inhibiting its interaction with the ribosome. Toxicol Appl Pharmacol. 2016 Nov 1;310:120-128. doi: 10.1016/j.taap.2016.09.004. Epub 2016 Sep 15. PubMed PMID: 27639428; PubMed Central PMCID: PMC5079286.
Basu D, Li XP, Kahn JN, May KL, Kahn PC, Tumer NE. The A1 Subunit of Shiga Toxin 2 Has Higher Affinity for Ribosomes and Higher Catalytic Activity than the A1 Subunit of Shiga Toxin 1. Infect Immun. 2016 Jan;84(1):149-61. doi: 10.1128/IAI.00994-15. Print 2016 Jan. PubMed PMID: 26483409; PubMed Central PMCID: PMC4693993.
Tumer NE. Introduction to the toxins special issue on plant toxins. Toxins (Basel). 2015 Nov;7(11):4503-6. doi: 10.3390/toxins7114503. PubMed PMID: 26837094; PubMed Central PMCID: PMC4663517.
McLaughlin JE, Bin-Umer MA, Widiez T, Finn D, McCormick S, Tumer NE. A Lipid Transfer Protein Increases the Glutathione Content and Enhances Arabidopsis Resistance to a Trichothecene Mycotoxin. PLoS One. 2015;10(6):e0130204. doi: 10.1371/journal.pone.0130204. eCollection 2015. PubMed PMID: 26057253; PubMed Central PMCID: PMC4461264.
Basu D, Tumer NE. Do the A subunits contribute to the differences in the toxicity of Shiga toxin 1 and Shiga toxin 2?. Toxins (Basel). 2015 Apr 29;7(5):1467-85. doi: 10.3390/toxins7051467. Review. PubMed PMID: 25938272; PubMed Central PMCID: PMC4448158.
Di R, Tumer NE. Pokeweed antiviral protein: its cytotoxicity mechanism and applications in plant disease resistance. Toxins (Basel). 2015 Mar 6;7(3):755-72. doi: 10.3390/toxins7030755. Review. PubMed PMID: 25756953; PubMed Central PMCID: PMC4379523.
Çakır B, Tumer NE. Arabidopsis Bax Inhibitor-1 inhibits cell death induced by pokeweed antiviral protein in Saccharomyces cerevisiae. Microb Cell. 2015 Feb 2;2(2):43-56. doi: 10.15698/mic2015.02.190. PubMed PMID: 28357275; PubMed Central PMCID: PMC5354556.
Yan Q, Li XP, Tumer NE. Wild type RTA and less toxic variants have distinct requirements for Png1 for their depurination activity and toxicity in Saccharomyces cerevisiae. PLoS One. 2014;9(12):e113719. doi: 10.1371/journal.pone.0113719. eCollection 2014. PubMed PMID: 25436896; PubMed Central PMCID: PMC4250064.
Bin-Umer MA, McLaughlin JE, Butterly MS, McCormick S, Tumer NE. Elimination of damaged mitochondria through mitophagy reduces mitochondrial oxidative stress and increases tolerance to trichothecenes. Proc Natl Acad Sci U S A. 2014 Aug 12;111(32):11798-803. doi: 10.1073/pnas.1403145111. Epub 2014 Jul 28. PubMed PMID: 25071194; PubMed Central PMCID: PMC4136610.
Grela P, Li XP, Tchórzewski M, Tumer NE. Functional divergence between the two P1-P2 stalk dimers on the ribosome in their interaction with ricin A chain. Biochem J. 2014 May 15;460(1):59-67. doi: 10.1042/BJ20140014. PubMed PMID: 24576056; PubMed Central PMCID: PMC4100481.
Di R, Tumer NE. An N-terminal fragment of yeast ribosomal protein L3 inhibits the cytotoxicity of pokeweed antiviral protein in Saccharomyces cerevisiae. Toxins (Basel). 2014 Apr 11;6(4):1349-61. doi: 10.3390/toxins6041349. PubMed PMID: 24732204; PubMed Central PMCID: PMC4014737.
Yu S, Park JG, Kahn JN, Tumer NE, Pang YP. Common pharmacophore of structurally distinct small-molecule inhibitors of intracellular retrograde trafficking of ribosome inactivating proteins. Sci Rep. 2013 Dec 2;3:3397. doi: 10.1038/srep03397. PubMed PMID: 24292269; PubMed Central PMCID: PMC3844963.
Li XP, Kahn PC, Kahn JN, Grela P, Tumer NE. Arginine residues on the opposite side of the active site stimulate the catalysis of ribosome depurination by ricin A chain by interacting with the P-protein stalk. J Biol Chem. 2013 Oct 18;288(42):30270-30284. doi: 10.1074/jbc.M113.510966. Epub 2013 Sep 3. PubMed PMID: 24003229; PubMed Central PMCID: PMC3798493.
May KL, Yan Q, Tumer NE. Targeting ricin to the ribosome. Toxicon. 2013 Jul;69:143-51. doi: 10.1016/j.toxicon.2013.02.001. Epub 2013 Feb 20. Review. PubMed PMID: 23454625; PubMed Central PMCID: PMC3672271.
Jetzt AE, Cheng JS, Li XP, Tumer NE, Cohick WS. A relatively low level of ribosome depurination by mutant forms of ricin toxin A chain can trigger protein synthesis inhibition, cell signaling and apoptosis in mammalian cells. Int J Biochem Cell Biol. 2012 Dec;44(12):2204-11. doi: 10.1016/j.biocel.2012.09.004. Epub 2012 Sep 12. PubMed PMID: 22982239; PubMed Central PMCID: PMC3496053.
Yan Q, Li XP, Tumer NE. N-glycosylation does not affect the catalytic activity of ricin a chain but stimulates cytotoxicity by promoting its transport out of the endoplasmic reticulum. Traffic. 2012 Nov;13(11):1508-21. doi: 10.1111/j.1600-0854.2012.01404.x. Epub 2012 Sep 7. PubMed PMID: 22882900; PubMed Central PMCID: PMC3465488.
May KL, Li XP, Martínez-Azorín F, Ballesta JP, Grela P, Tchórzewski M, Tumer NE. The P1/P2 proteins of the human ribosomal stalk are required for ribosome binding and depurination by ricin in human cells. FEBS J. 2012 Oct;279(20):3925-36. doi: 10.1111/j.1742-4658.2012.08752.x. Epub 2012 Sep 11. PubMed PMID: 22909382; PubMed Central PMCID: PMC3463775.
Park JG, Kahn JN, Tumer NE, Pang YP. Chemical structure of Retro-2, a compound that protects cells against ribosome-inactivating proteins. Sci Rep. 2012;2:631. doi: 10.1038/srep00631. Epub 2012 Sep 5. PubMed PMID: 22953052; PubMed Central PMCID: PMC3433688.
Tumer NE, Li XP. Interaction of ricin and Shiga toxins with ribosomes. Curr Top Microbiol Immunol. 2012;357:1-18. doi: 10.1007/82_2011_174. Review. PubMed PMID: 21910078; PubMed Central PMCID: PMC4103753.
Chiou JC, Li XP, Remacha M, Ballesta JP, Tumer NE. Shiga toxin 1 is more dependent on the P proteins of the ribosomal stalk for depurination activity than Shiga toxin 2. Int J Biochem Cell Biol. 2011 Dec;43(12):1792-801. doi: 10.1016/j.biocel.2011.08.018. Epub 2011 Sep 3. PubMed PMID: 21907821; PubMed Central PMCID: PMC3206194.
Di R, Kyu E, Shete V, Saidasan H, Kahn PC, Tumer NE. Identification of amino acids critical for the cytotoxicity of Shiga toxin 1 and 2 in Saccharomyces cerevisiae. Toxicon. 2011 Mar 15;57(4):525-39. doi: 10.1016/j.toxicon.2010.12.006. Epub 2010 Dec 22. PubMed PMID: 21184769; PubMed Central PMCID: PMC3055938.
Pierce M, Kahn JN, Chiou J, Tumer NE. Development of a quantitative RT-PCR assay to examine the kinetics of ribosome depurination by ribosome inactivating proteins using Saccharomyces cerevisiae as a model. RNA. 2011 Jan;17(1):201-10. doi: 10.1261/rna.2375411. Epub 2010 Nov 23. PubMed PMID: 21098653; PubMed Central PMCID: PMC3004061.
Li XP, Grela P, Krokowski D, Tchórzewski M, Tumer NE. Pentameric organization of the ribosomal stalk accelerates recruitment of ricin a chain to the ribosome for depurination. J Biol Chem. 2010 Dec 31;285(53):41463-71. doi: 10.1074/jbc.M110.171793. Epub 2010 Oct 25. PubMed PMID: 20974854; PubMed Central PMCID: PMC3009872.
McLaughlin JE, Bin-Umer MA, Tortora A, Mendez N, McCormick S, Tumer NE. A genome-wide screen in Saccharomyces cerevisiae reveals a critical role for the mitochondria in the toxicity of a trichothecene mycotoxin. Proc Natl Acad Sci U S A. 2009 Dec 22;106(51):21883-8. doi: 10.1073/pnas.0909777106. Epub 2009 Dec 9. PubMed PMID: 20007368; PubMed Central PMCID: PMC2799815.
Jetzt AE, Cheng JS, Tumer NE, Cohick WS. Ricin A-chain requires c-Jun N-terminal kinase to induce apoptosis in nontransformed epithelial cells. Int J Biochem Cell Biol. 2009 Dec;41(12):2503-10. doi: 10.1016/j.biocel.2009.08.007. Epub 2009 Aug 18. PubMed PMID: 19695342; PubMed Central PMCID: PMC2783365.
Li XP, Chiou JC, Remacha M, Ballesta JP, Tumer NE. A two-step binding model proposed for the electrostatic interactions of ricin a chain with ribosomes. Biochemistry. 2009 May 12;48(18):3853-63. doi: 10.1021/bi802371h. PubMed PMID: 19292477; PubMed Central PMCID: PMC2677637.
Chiou JC, Li XP, Remacha M, Ballesta JP, Tumer NE. The ribosomal stalk is required for ribosome binding, depurination of the rRNA and cytotoxicity of ricin A chain in Saccharomyces cerevisiae. Mol Microbiol. 2008 Dec;70(6):1441-52. doi: 10.1111/j.1365-2958.2008.06492.x. Epub 2008 Oct 30. PubMed PMID: 19019145; PubMed Central PMCID: PMC2637795.
Parikh BA, Tortora A, Li XP, Tumer NE. Ricin inhibits activation of the unfolded protein response by preventing splicing of the HAC1 mRNA. J Biol Chem. 2008 Mar 7;283(10):6145-53. doi: 10.1074/jbc.M707981200. Epub 2008 Jan 7. PubMed PMID: 18180297; PubMed Central PMCID: PMC3024914.
Synthesis and Structural Characterization of Ricin Inhibitors Targeting Ribosome Binding Using Fragment-Based Methods and Structure-Based Design. Journal of medicinal chemistry. . NIHMSID: NIHMS1947018.
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