Abstract
This communication describes a design of cell-permeable near-infrared fluorogenic substrates for imaging beta-lactamase expression in living mammalian cells. This design is based on fluorescence energy transfer resonance and utilizes a peracetylated d-glucosamine to facilitate the transport of the near-infrared probe across cell membranes. This new type of fluorogenic probe may also be applied to image gene expression in living animals.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Acetylation
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Animals
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Carbocyanines / chemical synthesis
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Carbocyanines / chemistry*
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Carbocyanines / pharmacokinetics
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Cell Line, Tumor
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Cell Membrane Permeability
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Cephalosporins / chemical synthesis
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Cephalosporins / chemistry*
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Cephalosporins / pharmacokinetics
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Fluorescence Resonance Energy Transfer
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Fluorescent Dyes / chemistry*
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Fluorescent Dyes / pharmacokinetics
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Glioma / enzymology
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Glioma / genetics
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Glioma / metabolism
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Glucosamine / analogs & derivatives
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Glucosamine / chemistry
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Rats
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Spectroscopy, Near-Infrared
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Transfection
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beta-Lactamases / analysis
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beta-Lactamases / biosynthesis
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beta-Lactamases / genetics
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beta-Lactamases / metabolism*
Substances
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Carbocyanines
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Cephalosporins
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Fluorescent Dyes
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cyanine dye 5
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beta-Lactamases
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beta-lactamase TEM-1
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Glucosamine