T-protein of the glycine decarboxylase multienzyme complex: evidence for partial similarity to formyltetrahydrofolate synthetase

S Kopriva; S R Turner; S Rawsthorne; H Bauwe

doi:10.1007/BF00020895

T-protein of the glycine decarboxylase multienzyme complex: evidence for partial similarity to formyltetrahydrofolate synthetase

Plant Mol Biol. 1995 Mar;27(6):1215-20. doi: 10.1007/BF00020895.

Authors

S Kopriva¹, S R Turner, S Rawsthorne, H Bauwe

Affiliation

¹ Department of Molecular Cell Biology, Institute of Plant Genetics and Crop Plant Research, Gatersleben, Germany.

PMID: 7766903
DOI: 10.1007/BF00020895

Abstract

We have isolated and sequenced cDNA clones encoding T-protein of the glycine decarboxylase complex from three plant species, Flaveria pringlei, Solanum tuberosum and Pisum sativum. The predicted amino acid sequences of these clones are at least 87% identical and all are similar to the predicted sequences of the bovine, human, chicken and Escherichia coli T-proteins. Alignment of all these sequences revealed conserved domains, one of which showed a significant similarity to a part of the formyltetrahydrofolate synthetases from procaryotes and eucaryotes. This suggests that the T-protein sequence is not as unique as previously thought.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Oxidoreductases / genetics*
Amino Acid Sequence
Animals
DNA, Complementary
Formate-Tetrahydrofolate Ligase / genetics*
Glycine Decarboxylase Complex
Glycine Dehydrogenase (Decarboxylating)
Humans
Molecular Sequence Data
Multienzyme Complexes / genetics*
Phosphoproteins / genetics*
Plant Proteins / genetics*
Plants / enzymology
Plants / genetics*
Sequence Homology, Amino Acid

Substances

DNA, Complementary
Multienzyme Complexes
Phosphoproteins
Plant Proteins
T-substrate protein, Triticum aestivum
Amino Acid Oxidoreductases
Glycine Decarboxylase Complex
Glycine Dehydrogenase (Decarboxylating)
Formate-Tetrahydrofolate Ligase

Associated data

GENBANK/Z25858
GENBANK/Z25861
GENBANK/Z25862