Sequence and molecular analysis of the rpoA cluster genes from Xanthomonas campestris pv. campestris

Biochim Biophys Acta. 2000 Jul 24;1492(2-3):553-9. doi: 10.1016/s0167-4781(00)00143-3.

Abstract

The Xanthomonas campestris rpsM (S13)-rpsK (S11)-rpsD (S4)-rpoA (alpha)-rplQ (L17) cluster, encoding RNA polymerase alpha-subunit and four ribosomal proteins, reside in a 3164-bp DNA region. The N-terminal sequence of the authentic alpha-protein determined chemically matches that predicted from the nucleotide sequence. rplQ is monocistronic, instead of being co-transcribed with the other genes as in Escherichia coli. Antiserum against the His-tagged alpha-protein cross-reacted with the E. coli alpha-protein.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • DNA, Bacterial / analysis
  • DNA-Directed RNA Polymerases / genetics*
  • Molecular Sequence Data
  • Multigene Family
  • Restriction Mapping
  • Sequence Homology, Amino Acid
  • Xanthomonas campestris / genetics*

Substances

  • DNA, Bacterial
  • DNA-Directed RNA Polymerases
  • RNA polymerase alpha subunit