Abstract
The voltage-dependent anion channels (VDACs), prominently localized in the outer mitochondrial membrane, play important roles in the metabolite exchange, energy metabolism and mitochondria-mediated apoptosis process in mammalian cells. However, relatively little is known about the functions of VDACs in plants. To further investigate the function of AtVDAC1 in Arabidopsis, we analyzed a T-DNA insertion line for the AtVDAC1 gene. The knock-out mutant atvdac1 showed reduced seed set due to a large number of undeveloped ovules in siliques. Genetic analyses indicated that the mutation of AtVDAC1 affected female fertility and belonged to a sporophytic mutation. Abnormal ovules in the process of female gametogenesis were observed using a confocal laser scanning microscope. Interestingly, both mitochondrial transmembrane potential (ΔΨ) and ATP synthesis rate were obviously reduced in the mitochondria isolated from atvdac1 plants.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenosine Triphosphate / metabolism
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Arabidopsis* / genetics
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Arabidopsis* / growth & development
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Arabidopsis* / metabolism
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DNA, Bacterial / genetics
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Energy Metabolism / genetics*
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Gametogenesis, Plant / genetics
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Gene Expression Regulation, Plant
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Membrane Potential, Mitochondrial / genetics
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Mitochondria / genetics
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Mitochondria / physiology*
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Ovule / genetics
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Ovule / growth & development*
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Plant Development / genetics*
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Plants, Genetically Modified
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Voltage-Dependent Anion Channel 1 / genetics
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Voltage-Dependent Anion Channel 1 / physiology*
Substances
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DNA, Bacterial
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T-DNA
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Adenosine Triphosphate
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Voltage-Dependent Anion Channel 1
Grants and funding
This work was supported by grants from the National Natural Science Foundation of China (Project 31071259). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.