Reconstitution of both steps of Saccharomyces cerevisiae splicing with purified spliceosomal components.
Warkocki Z, Odenwälder P, Schmitzová J, Platzmann F, Stark H, Urlaub H, Ficner R, Fabrizio P, Lührmann R.
Warkocki Z, et al.
Nat Struct Mol Biol. 2009 Dec;16(12):1237-43. doi: 10.1038/nsmb.1729. Epub 2009 Nov 22.
Nat Struct Mol Biol. 2009.
PMID: 19935684
Spliceosomes stalled before step 1 of this process were purified to near-homogeneity from a temperature-sensitive mutant of the RNA helicase Prp2, compositionally defined, and shown to catalyze efficient step 1 when supplemented with recombinant Prp2, Spp2 and Cwc25, there …
Spliceosomes stalled before step 1 of this process were purified to near-homogeneity from a temperature-sensitive mutant of the RNA helicase …