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Table representation of search results timeline featuring number of search results per year.

Year Number of Results
2009 2
2010 1
2011 1
2012 2
2013 6
2014 1
2015 1
2016 3
2017 3
2018 1
2019 1
2022 2
2023 1
2024 0

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22 results

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Page 1
Cwc25 is a novel splicing factor required after Prp2 and Yju2 to facilitate the first catalytic reaction.
Chiu YF, Liu YC, Chiang TW, Yeh TC, Tseng CK, Wu NY, Cheng SC. Chiu YF, et al. Mol Cell Biol. 2009 Nov;29(21):5671-8. doi: 10.1128/MCB.00773-09. Epub 2009 Aug 24. Mol Cell Biol. 2009. PMID: 19704000 Free PMC article.
We show here that Cwc25 is neither tightly associated with NTC nor required for spliceosome activation but is required for the first catalytic reaction. The affinity-purified spliceosome formed in Cwc25-depleted extracts contained only pre-mRNA and could be chased i …
We show here that Cwc25 is neither tightly associated with NTC nor required for spliceosome activation but is required for the first …
A central role of Cwc25 in spliceosome dynamics during the catalytic phase of pre-mRNA splicing.
Tseng CK, Chung CS, Chen HC, Cheng SC. Tseng CK, et al. RNA. 2017 Apr;23(4):546-556. doi: 10.1261/rna.059204.116. Epub 2017 Jan 5. RNA. 2017. PMID: 28057857 Free PMC article.
Both reactions are inhibited when Cwc25 is added back, presumably due to the stabilization of first-step conformation. Prp16 is dispensable for the second reaction when splicing is carried out under conditions that destabilize Cwc25. We also show that the purified p …
Both reactions are inhibited when Cwc25 is added back, presumably due to the stabilization of first-step conformation. Prp16 is dispe …
Dynamic Contacts of U2, RES, Cwc25, Prp8 and Prp45 Proteins with the Pre-mRNA Branch-Site and 3' Splice Site during Catalytic Activation and Step 1 Catalysis in Yeast Spliceosomes.
Schneider C, Agafonov DE, Schmitzová J, Hartmuth K, Fabrizio P, Lührmann R. Schneider C, et al. PLoS Genet. 2015 Sep 22;11(9):e1005539. doi: 10.1371/journal.pgen.1005539. eCollection 2015. PLoS Genet. 2015. PMID: 26393790 Free PMC article.
A comparison of the B(act) crosslinking pattern versus that of B* and C complexes revealed that U2 and RES protein interactions with the intron are dynamic. Upon step 1 catalysis, Cwc25 contacts with the branch-site region, and enhanced crosslinks of Prp8 and Prp45 with nu …
A comparison of the B(act) crosslinking pattern versus that of B* and C complexes revealed that U2 and RES protein interactions with the int …
An ATP-independent role for Prp16 in promoting aberrant splicing.
Chung CS, Wai HL, Kao CY, Cheng SC. Chung CS, et al. Nucleic Acids Res. 2023 Nov 10;51(20):10815-10828. doi: 10.1093/nar/gkad861. Nucleic Acids Res. 2023. PMID: 37858289 Free PMC article.
Furthermore, we provide evidence that these functions of Prp16 are mediated through the step-one factor Cwc25. The DExD/H-box protein Prp16 has a well-established role in proofreading the 5' splice site and the branch site of precursor mRNA through ATP hydrolysis to ensure …
Furthermore, we provide evidence that these functions of Prp16 are mediated through the step-one factor Cwc25. The DExD/H-box protein …
Structure of a human catalytic step I spliceosome.
Zhan X, Yan C, Zhang X, Lei J, Shi Y. Zhan X, et al. Science. 2018 Feb 2;359(6375):537-545. doi: 10.1126/science.aar6401. Epub 2018 Jan 4. Science. 2018. PMID: 29301961
Compared with the Saccharomyces cerevisiae C complex, the human complex contains 11 additional proteins. The step I splicing factors CCDC49 and CCDC94 (Cwc25 and Yju2 in S. cerevisiae, respectively) closely interact with the DEAH-family adenosine triphosphatase/helicase Pr …
Compared with the Saccharomyces cerevisiae C complex, the human complex contains 11 additional proteins. The step I splicing factors CCDC49 …
Structures of the Catalytically Activated Yeast Spliceosome Reveal the Mechanism of Branching.
Wan R, Bai R, Yan C, Lei J, Shi Y. Wan R, et al. Cell. 2019 Apr 4;177(2):339-351.e13. doi: 10.1016/j.cell.2019.02.006. Epub 2019 Mar 14. Cell. 2019. PMID: 30879786 Free article.
The duplex between U2 small nuclear RNA (snRNA) and the branch point sequence (BPS) is discretely away from the 5'-splice site (5'SS) in the three B() complexes that are devoid of the step I splicing factors Yju2 and Cwc25. Recruitment of Yju2 into the active site brings t …
The duplex between U2 small nuclear RNA (snRNA) and the branch point sequence (BPS) is discretely away from the 5'-splice site (5'SS) in the …
DEAH-box ATPase Prp16 has dual roles in remodeling of the spliceosome in catalytic steps.
Tseng CK, Liu HL, Cheng SC. Tseng CK, et al. RNA. 2011 Jan;17(1):145-54. doi: 10.1261/rna.2459611. Epub 2010 Nov 22. RNA. 2011. PMID: 21098140 Free PMC article.
The detailed mechanism of their action was not well understood until recently, when Prp2 was shown to be required for the release of U2 components SF3a and SF3b, presumably to allow the binding of Cwc25 to promote the first transesterification reaction. We show here that …
The detailed mechanism of their action was not well understood until recently, when Prp2 was shown to be required for the release of U2 comp …
Cryo-EM structure of the spliceosome immediately after branching.
Galej WP, Wilkinson ME, Fica SM, Oubridge C, Newman AJ, Nagai K. Galej WP, et al. Nature. 2016 Sep 8;537(7619):197-201. doi: 10.1038/nature19316. Epub 2016 Jul 26. Nature. 2016. PMID: 27459055 Free PMC article.
Non-Watson-Crick interactions between the branch helix and 5'-splice site dock the branch adenosine into the active site, while intron nucleotides +3 to +6 base-pair with the U6 snRNA ACAGAGA sequence. Isy1 and the step-one factors Yju2 and Cwc25 stabilize docking of the b …
Non-Watson-Crick interactions between the branch helix and 5'-splice site dock the branch adenosine into the active site, while intron nucle …
Reconstitution of both steps of Saccharomyces cerevisiae splicing with purified spliceosomal components.
Warkocki Z, Odenwälder P, Schmitzová J, Platzmann F, Stark H, Urlaub H, Ficner R, Fabrizio P, Lührmann R. Warkocki Z, et al. Nat Struct Mol Biol. 2009 Dec;16(12):1237-43. doi: 10.1038/nsmb.1729. Epub 2009 Nov 22. Nat Struct Mol Biol. 2009. PMID: 19935684
Spliceosomes stalled before step 1 of this process were purified to near-homogeneity from a temperature-sensitive mutant of the RNA helicase Prp2, compositionally defined, and shown to catalyze efficient step 1 when supplemented with recombinant Prp2, Spp2 and Cwc25, there …
Spliceosomes stalled before step 1 of this process were purified to near-homogeneity from a temperature-sensitive mutant of the RNA helicase …
Structure of a spliceosome remodelled for exon ligation.
Fica SM, Oubridge C, Galej WP, Wilkinson ME, Bai XC, Newman AJ, Nagai K. Fica SM, et al. Nature. 2017 Feb 16;542(7641):377-380. doi: 10.1038/nature21078. Epub 2017 Jan 11. Nature. 2017. PMID: 28076345 Free PMC article.
Recently reported structures of the spliceosomal C complex with the cleaved 5' exon and lariat-3'-exon bound to the catalytic centre revealed that branching-specific factors such as Cwc25 lock the branch helix into position for nucleophilic attack of the branch adenosine a …
Recently reported structures of the spliceosomal C complex with the cleaved 5' exon and lariat-3'-exon bound to the catalytic centre reveale …
22 results