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Page 1
Culture with Matrigel inhibits development of mouse zygotes.
Dawson KM, Baltz JM, Claman P. Dawson KM, et al. J Assist Reprod Genet. 1997 Oct;14(9):543-8. doi: 10.1023/a:1021135612118. J Assist Reprod Genet. 1997. PMID: 9401874 Free PMC article.
METHODS: Mouse embryo development was assessed with or without Matrigel in KSOM medium, which supports the development of blocking strain zygotes in vitro, and in human tubal fluid (HTF) medium, which normally does not but which is used for huma …
METHODS: Mouse embryo development was assessed with or without Matrigel in KSOM medium, which supports the de …
Can Matrigel substitute for Vero cells in promoting the in-vitro development of mouse embryos?
Carnegie J, Claman P, Lawrence C, Cabaca O. Carnegie J, et al. Hum Reprod. 1995 Mar;10(3):636-41. doi: 10.1093/oxfordjournals.humrep.a136002. Hum Reprod. 1995. PMID: 7782445
In conclusion, Vero cells improved the rate of development of mouse embryos to hatched blastocysts during serum-free culture. ...Further studies on the means whereby Matrigel promotes early embryonic development (e.g. appropriate c …
In conclusion, Vero cells improved the rate of development of mouse embryos to hatched blastocysts during serum-free …
Methods for culturing mouse and human embryonic stem cells.
Lin S, Talbot P. Lin S, et al. Methods Mol Biol. 2011;690:31-56. doi: 10.1007/978-1-60761-962-8_2. Methods Mol Biol. 2011. PMID: 21042983
Mouse embryonic stem cells (mESCs) were first derived and cultured almost 30 years ago and ever since have been valuable tools for creating knockout mice and for studying early mammalian development. ...The purpose of this chapter is to provide basic p …
Mouse embryonic stem cells (mESCs) were first derived and cultured almost 30 years ago and ever since have been valuable tools …
Paracrine and epigenetic control of trophectoderm differentiation from human embryonic stem cells: the role of bone morphogenic protein 4 and histone deacetylases.
Erb TM, Schneider C, Mucko SE, Sanfilippo JS, Lowry NC, Desai MN, Mangoubi RS, Leuba SH, Sammak PJ. Erb TM, et al. Stem Cells Dev. 2011 Sep;20(9):1601-14. doi: 10.1089/scd.2010.0281. Epub 2011 Mar 17. Stem Cells Dev. 2011. PMID: 21204619 Free PMC article.
Our understanding of paracrine and epigenetic control of trophectoderm (TE) differentiation is limited by available models of preimplantation human development. Simple, defined media for selective TE differentiation of human embryonic stem cells (hESCs) were …
Our understanding of paracrine and epigenetic control of trophectoderm (TE) differentiation is limited by available models of preimplantatio …
Variations in humanized and defined culture conditions supporting derivation of new human embryonic stem cell lines.
Fletcher JM, Ferrier PM, Gardner JO, Harkness L, Dhanjal S, Serhal P, Harper J, Delhanty J, Brownstein DG, Prasad YR, Lebkowski J, Mandalam R, Wilmut I, De Sousa PA. Fletcher JM, et al. Cloning Stem Cells. 2006 Winter;8(4):319-34. doi: 10.1089/clo.2006.8.319. Cloning Stem Cells. 2006. PMID: 17196096
., free of animal sourced reagents) and ultimately chemically defined culture systems for human embryo stem cell (hESC) isolation and culture is of importance to improving their efficacy and safety in research and therapeutic applications. ...With this integr …
., free of animal sourced reagents) and ultimately chemically defined culture systems for human embryo stem cell (hESC) isolat …
Expression and localization of thioredoxin during early implantation in the marmoset monkey.
Lopata A, Sibson MC, Enders AC, Bloomfield KL, Gregory MS, Trapani GD, Perkins AV, Tonissen KF, Clarke FM. Lopata A, et al. Mol Hum Reprod. 2001 Dec;7(12):1159-65. doi: 10.1093/molehr/7.12.1159. Mol Hum Reprod. 2001. PMID: 11719593
Thioredoxin is a powerful redox protein expressed in invasive cytotrophoblasts and essential for blastocyst implantation in mice. Isolated marmoset thioredoxin cDNA showed that the deduced amino acid sequence differed from the human sequence by four amino acids. ... …
Thioredoxin is a powerful redox protein expressed in invasive cytotrophoblasts and essential for blastocyst implantation in mice …
Complete and unidirectional conversion of human embryonic stem cells to trophoblast by BMP4.
Amita M, Adachi K, Alexenko AP, Sinha S, Schust DJ, Schulz LC, Roberts RM, Ezashi T. Amita M, et al. Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):E1212-21. doi: 10.1073/pnas.1303094110. Epub 2013 Mar 14. Proc Natl Acad Sci U S A. 2013. PMID: 23493551 Free PMC article.
Here we confirm that hESC grown under the standard conditions on a medium conditioned by mouse embryonic fibroblasts in the presence of BMP4 and absence of FGF2 on a Matrigel substratum rapidly convert to an epithelium that is largely KRT7(+) within 48 h, wit …
Here we confirm that hESC grown under the standard conditions on a medium conditioned by mouse embryonic fibroblasts in the pr …
Heightened potency of human pluripotent stem cell lines created by transient BMP4 exposure.
Yang Y, Adachi K, Sheridan MA, Alexenko AP, Schust DJ, Schulz LC, Ezashi T, Roberts RM. Yang Y, et al. Proc Natl Acad Sci U S A. 2015 May 5;112(18):E2337-46. doi: 10.1073/pnas.1504778112. Epub 2015 Apr 13. Proc Natl Acad Sci U S A. 2015. PMID: 25870291 Free PMC article.
They form well-differentiated teratomas in immune-compromised mice that secrete human chorionic gonadotropin (hCG) into the host mouse and include small areas of trophoblast-like cells. ...Together, these data suggest that the cell lines exhibit totipotent potential …
They form well-differentiated teratomas in immune-compromised mice that secrete human chorionic gonadotropin (hCG) into the host m …
Differentiation of endothelial cells derived from mouse embryoid bodies: a possible in vitro vasculogenesis model.
Kim GD, Kim GJ, Seok JH, Chung HM, Chee KM, Rhee GS. Kim GD, et al. Toxicol Lett. 2008 Aug 28;180(3):166-73. doi: 10.1016/j.toxlet.2008.05.023. Epub 2008 Jun 11. Toxicol Lett. 2008. PMID: 18590808
Mouse embryonic stem cells (mES cells), which are pluripotent and self-renewal cells, are derived from the inner cell mass of mouse blastocysts. ...EBs were formed for 3 days using hanging drop cultures and plated on gelatin-coated plates in endothelia …
Mouse embryonic stem cells (mES cells), which are pluripotent and self-renewal cells, are derived from the inner cell mass of …
A feeder-free culture using autogeneic conditioned medium for undifferentiated growth of human embryonic stem cells: comparative expression profiles of mRNAs, microRNAs and proteins among different feeders and conditioned media.
Tsai ZY, Singh S, Yu SL, Chou CH, Li SS. Tsai ZY, et al. BMC Cell Biol. 2010 Oct 12;11:76. doi: 10.1186/1471-2121-11-76. BMC Cell Biol. 2010. PMID: 20937144 Free PMC article.
BACKGROUND: Human embryonic stem (hES) cell lines were derived from the inner cell mass of human blastocysts, and were cultured on mouse embryonic fibroblast (MEF) feeder to maintain undifferentiated growth, extensive renewal capacity, and pluripotency …
BACKGROUND: Human embryonic stem (hES) cell lines were derived from the inner cell mass of human blastocysts, and were culture …