ERK1/2 mediates lung adenocarcinoma cell proliferation and autophagy induced by apelin-13

Li Yang; Tao Su; Deguan Lv; Feng Xie; Wei Liu; Jiangang Cao; Irshad Ali Sheikh; Xuping Qin; Lanfang Li; Linxi Chen

doi:10.1093/abbs/gmt140

ERK1/2 mediates lung adenocarcinoma cell proliferation and autophagy induced by apelin-13

Acta Biochim Biophys Sin (Shanghai). 2014 Feb;46(2):100-11. doi: 10.1093/abbs/gmt140. Epub 2013 Dec 29.

Authors

Li Yang¹, Tao Su, Deguan Lv, Feng Xie, Wei Liu, Jiangang Cao, Irshad Ali Sheikh, Xuping Qin, Lanfang Li, Linxi Chen

Affiliation

¹ Learning Key Laboratory for Pharmaco-proteomics, Institute of Pharmacy and Pharmacology, University of South China, Hengyang 421001, China.

PMID: 24374773
DOI: 10.1093/abbs/gmt140

Abstract

The aim of this study was to investigate the role of apelin in the cell proliferation and autophagy of lung adenocarcinoma. The over-expression of APJ in lung adenocarcinoma was detected by immunohistochemistry, while plasma apelin level in lung cancer patients was measured by enzyme-linked immunosorbent assay. Our findings revealed that apelin-13 significantly increased the phosphorylation of ERK1/2, the expression of cyclin D1, microtubule-associated protein 1 light chain 3A/B (LC3A/B), and beclin1, and confirmed that apelin-13 promoted A549 cell proliferation and induced A549 cell autophagy via ERK1/2 signaling. Moreover, there are pores on the surface of human lung adenocarcinoma cell line A549 and apelin-13 causes cell surface smooth and glossy as observed under atomic force microscopy. These results suggested that ERK1/2 signaling pathway mediates apelin-13-induced lung adenocarcinoma cell proliferation and autophagy. Under our experimental condition, autophagy associated with 3-methyladenine was not involved in cell proliferation.

Keywords: APJ; ERK1/2; apelin; autophagy; lung adenocarcinoma; proliferation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adenocarcinoma / pathology
Adenocarcinoma / physiopathology*
Adenocarcinoma of Lung
Apoptosis Regulatory Proteins / biosynthesis
Autophagy / drug effects*
Beclin-1
Cell Cycle Checkpoints / drug effects
Cell Line, Tumor
Cell Proliferation / drug effects
Cyclin D1
Extracellular Signal-Regulated MAP Kinases / physiology*
Flavonoids / pharmacology
Humans
Intercellular Signaling Peptides and Proteins / biosynthesis*
Intercellular Signaling Peptides and Proteins / blood
Intercellular Signaling Peptides and Proteins / physiology
Lung Neoplasms / pathology
Lung Neoplasms / physiopathology*
Membrane Proteins / biosynthesis
Microtubule-Associated Proteins / biosynthesis
Phosphorylation / drug effects
Signal Transduction

Substances

Apoptosis Regulatory Proteins
BECN1 protein, human
Beclin-1
Flavonoids
Intercellular Signaling Peptides and Proteins
MAP1LC3A protein, human
MAP1LC3B protein, human
Membrane Proteins
Microtubule-Associated Proteins
apelin-13 peptide
Cyclin D1
Extracellular Signal-Regulated MAP Kinases
2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one