Arabidopsis H+-PPase AVP1 regulates auxin-mediated organ development

Science. 2005 Oct 7;310(5745):121-5. doi: 10.1126/science.1115711.

Abstract

The transport of auxin controls developmental events in plants. Here, we report that in addition to maintaining vacuolar pH, the H+-pyrophosphatase, AVP1, controls auxin transport and consequently auxin-dependent development. AVP1 overexpression results in increased cell division at the onset of organ formation, hyperplasia, and increased auxin transport. In contrast, avp1-1 null mutants have severely disrupted root and shoot development and reduced auxin transport. Changes in the expression of AVP1 affect the distribution and abundance of the P-adenosine triphosphatase and Pinformed 1 auxin efflux facilitator, two proteins implicated in auxin distribution. Thus, AVP1 facilitates the auxin fluxes that regulate organogenesis.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Adenosine Triphosphatases / metabolism
  • Arabidopsis / cytology
  • Arabidopsis / genetics
  • Arabidopsis / growth & development*
  • Arabidopsis / metabolism*
  • Arabidopsis Proteins / metabolism
  • Biological Transport
  • Cell Count
  • Cell Proliferation
  • Cell Shape
  • Cell Wall / metabolism
  • Hydrogen-Ion Concentration
  • In Situ Hybridization
  • Indoleacetic Acids / metabolism*
  • Indoleacetic Acids / pharmacology
  • Inorganic Pyrophosphatase / genetics
  • Inorganic Pyrophosphatase / metabolism*
  • Membrane Transport Proteins / metabolism
  • Meristem / metabolism
  • Microsomes / metabolism
  • Mutation
  • Plant Leaves / cytology
  • Plant Leaves / growth & development
  • Plant Leaves / metabolism
  • Plant Roots / cytology
  • Plant Roots / growth & development
  • Plant Roots / metabolism
  • Proton Pumps / genetics
  • Proton Pumps / metabolism*
  • RNA Interference
  • Signal Transduction
  • Transformation, Genetic

Substances

  • Arabidopsis Proteins
  • Indoleacetic Acids
  • Membrane Transport Proteins
  • PIN1 protein, Arabidopsis
  • Proton Pumps
  • indoleacetic acid
  • Adenosine Triphosphatases
  • AVP1 protein, Arabidopsis
  • Inorganic Pyrophosphatase