Abstract
The transport of auxin controls developmental events in plants. Here, we report that in addition to maintaining vacuolar pH, the H+-pyrophosphatase, AVP1, controls auxin transport and consequently auxin-dependent development. AVP1 overexpression results in increased cell division at the onset of organ formation, hyperplasia, and increased auxin transport. In contrast, avp1-1 null mutants have severely disrupted root and shoot development and reduced auxin transport. Changes in the expression of AVP1 affect the distribution and abundance of the P-adenosine triphosphatase and Pinformed 1 auxin efflux facilitator, two proteins implicated in auxin distribution. Thus, AVP1 facilitates the auxin fluxes that regulate organogenesis.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Adenosine Triphosphatases / metabolism
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Arabidopsis / cytology
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Arabidopsis / genetics
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Arabidopsis / growth & development*
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Arabidopsis / metabolism*
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Arabidopsis Proteins / metabolism
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Biological Transport
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Cell Count
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Cell Proliferation
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Cell Shape
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Cell Wall / metabolism
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Hydrogen-Ion Concentration
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In Situ Hybridization
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Indoleacetic Acids / metabolism*
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Indoleacetic Acids / pharmacology
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Inorganic Pyrophosphatase / genetics
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Inorganic Pyrophosphatase / metabolism*
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Membrane Transport Proteins / metabolism
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Meristem / metabolism
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Microsomes / metabolism
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Mutation
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Plant Leaves / cytology
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Plant Leaves / growth & development
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Plant Leaves / metabolism
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Plant Roots / cytology
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Plant Roots / growth & development
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Plant Roots / metabolism
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Proton Pumps / genetics
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Proton Pumps / metabolism*
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RNA Interference
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Signal Transduction
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Transformation, Genetic
Substances
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Arabidopsis Proteins
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Indoleacetic Acids
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Membrane Transport Proteins
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PIN1 protein, Arabidopsis
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Proton Pumps
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indoleacetic acid
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Adenosine Triphosphatases
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AVP1 protein, Arabidopsis
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Inorganic Pyrophosphatase