Isolation of an iron-binding compound from Pseudomonas aeruginosa

J Bacteriol. 1979 Jan;137(1):357-64. doi: 10.1128/jb.137.1.357-364.1979.

Abstract

An iron-binding compound was isolated from ethyl acetate extracts of culture supernatant fluids of Pseudomonas aeruginosa and was purified by successive paper and thin-layer chromatographic procedures. The purified compound was characterized by UV, visible, infrared, and fluorescence spectroscopy. The compound possesses phenolic characteristics, with little or no similarity to dihydroxybenzoates and no indication of a hydroxamate group. P. aeruginosa synthesized the compound during active growth in culture media containing less than 5 X 10(-6) M added FeCl3. When added to iron-poor cultures of P. aeruginosa, the compound promoted the growth of the bacterium and also reversed growth inhibition by the iron chelator ethylenediamine-di-(o-hydroxyphenylacetic acid).

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Ethylenediamines / antagonists & inhibitors
  • Iron Chelating Agents / biosynthesis
  • Iron Chelating Agents / isolation & purification*
  • Iron Chelating Agents / pharmacology
  • Pseudomonas aeruginosa / analysis*
  • Pseudomonas aeruginosa / drug effects
  • Pseudomonas aeruginosa / growth & development

Substances

  • Ethylenediamines
  • Iron Chelating Agents