Gene for the catalytic subunit of mouse DNA-dependent protein kinase maps to the scid locus

Proc Natl Acad Sci U S A. 1995 Nov 7;92(23):10792-5. doi: 10.1073/pnas.92.23.10792.

Abstract

The gene encoding the catalytic subunit of DNA-dependent protein kinase (DNA-PKcs) has been proposed recently as a candidate gene for the mouse severe combined immune deficiency (scid) locus. We have used a partial cDNA clone for human DNA-PKcs to map the mouse homologue using a large interspecific backcross panel. We found that the mouse gene for DNA-PKcs does not recombine with scid, consistent with the hypothesis that scid is a mutation in the mouse gene for DNA-PKcs.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Base Sequence
  • Chromosome Mapping*
  • Crosses, Genetic
  • DNA-Activated Protein Kinase
  • DNA-Binding Proteins*
  • Mice
  • Mice, SCID / genetics*
  • Molecular Sequence Data
  • Muridae
  • Mutation*
  • Polymorphism, Genetic
  • Protein Serine-Threonine Kinases / genetics*
  • Recombination, Genetic
  • Severe Combined Immunodeficiency / genetics*

Substances

  • DNA-Binding Proteins
  • DNA-Activated Protein Kinase
  • Protein Serine-Threonine Kinases