Deletion of methylglyoxal synthase gene (mgsA) increased sugar co-metabolism in ethanol-producing Escherichia coli

L P Yomano; S W York; K T Shanmugam; L O Ingram

doi:10.1007/s10529-009-0011-8

Deletion of methylglyoxal synthase gene (mgsA) increased sugar co-metabolism in ethanol-producing Escherichia coli

Biotechnol Lett. 2009 Sep;31(9):1389-98. doi: 10.1007/s10529-009-0011-8. Epub 2009 May 21.

Authors

L P Yomano¹, S W York, K T Shanmugam, L O Ingram

Affiliation

¹ Department of Microbiology and Cell Science, University of Florida, Gainesville, FL 32611, USA.

Abstract

The use of lignocellulose as a source of sugars for bioproducts requires the development of biocatalysts that maximize product yields by fermenting mixtures of hexose and pentose sugars to completion. In this study, we implicate mgsA encoding methylglyoxal synthase (and methylglyoxal) in the modulation of sugar metabolism. Deletion of this gene (strain LY168) resulted in the co-metabolism of glucose and xylose, and accelerated the metabolism of a 5-sugar mixture (mannose, glucose, arabinose, xylose and galactose) to ethanol.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Carbohydrate Metabolism*
Carbon-Oxygen Lyases / genetics*
DNA, Bacterial / chemistry
DNA, Bacterial / genetics
Escherichia coli / enzymology*
Escherichia coli / metabolism*
Escherichia coli Proteins / genetics*
Ethanol / metabolism*
Gene Deletion*
Lignin / metabolism
Molecular Sequence Data
Sequence Analysis, DNA

Substances

DNA, Bacterial
Escherichia coli Proteins
lignocellulose
Ethanol
Lignin
Carbon-Oxygen Lyases
methylglyoxal synthase

Associated data

GENBANK/FJ015153
GENBANK/FJ015154
GENBANK/FJ032247