cas1 multifunctional nuclease Cas1 [Escherichia coli str. K-12 substr. MG1655] - Gene

Summary

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Gene symbol: cas1
Gene description: multifunctional nuclease Cas1
Primary source: ASAP:ABE-0009038
Locus tag: b2755
See related: ECOCYC:G7425
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage: Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as: ECK2750; ygbT
Summary: DUF48 member. [More information is available at EcoGene: EG13114]. Cas1: [More information is available at EcoCyc: G7425].
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Genomic context

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Sequence:: NC_000913.3 (2878855..2879772, complement)

NC_000913.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

A mini-integrase comprising the type V-C Cas1 protein alone catalyzes DNA integration with a preference for short (17- to 19-base-pair) DNA fragments in a two-protein-type V-C CRISPR system.
Title: A Functional Mini-Integrase in a Two-Protein-type V-C CRISPR System.
These results explain how the Cas1-Cas2 CRISPR integrase recognizes a sequence-dependent DNA structure to ensure site-selective CRISPR array expansion during the initial step of bacterial adaptive immunity.
Title: Structures of the CRISPR genome integration complex.
in Escherichia coli cells undergoing primed adaptation, spacer-sized fragments of foreign DNA are associated with Cas1.
Title: Spacer-length DNA intermediates are associated with Cas1 in cells undergoing primed CRISPR adaptation.
to form a long-lived ternary complex containing Cas1-Cas2, the acceptor DNA must carry a CRISPR locus, and the protospacer must not contain 3-single-stranded overhangs longer than 5 bases.
Title: DNA binding specificities of Escherichia coli Cas1-Cas2 integrase drive its recruitment at the CRISPR locus.
Asymmetric positioning of Cas1-Cas2 complex and Integration Host Factor induced DNA bending guide the unidirectional homing of protospacer in CRISPR-Cas type I-E system.
Title: Asymmetric positioning of Cas1-2 complex and Integration Host Factor induced DNA bending guide the unidirectional homing of protospacer in CRISPR-Cas type I-E system.
Cas1 from both Escherichia coli and Sulfolobus solfataricus display sequence specific activity, with a clear preference for the nucleotides flanking the integration site at the leader-repeat 1 boundary of the CRISPR locus.
Title: Intrinsic sequence specificity of the Cas1 integrase directs new spacer acquisition.
These results establish Cas1-Cas2 as a trans-acting factor necessary for the recruitment and regulation of Cas3 at escape targets. Based on findings, a mechanistic framework is proposed describing how Cascade, Cas1, Cas2, and Cas3 work together to process and disable foreign genetic elements.
Title: Surveillance and Processing of Foreign DNA by the Escherichia coli CRISPR-Cas System.
To understand the molecular mechanisms of spacer acquisition, the crystal structure of E. coli Cas1-Cas2 bound with dual-forked DNA was determined; structure highlights the mechanistic principles related to new spacer acquisition, the protospacer DNA captured by Cas1-Cas2 adopts a dual-forked form, with the 3' overhangs of the protospacer essential for new spacer.
Title: Structural and Mechanistic Basis of PAM-Dependent Spacer Acquisition in CRISPR-Cas Systems.
CRISPR-Cas functions by integrating phage sequences into CRISPR loci on the host genome.
Title: Parasite Exposure Drives Selective Evolution of Constitutive versus Inducible Defense.
Cas1-Cas2 complexes specify sites of CRISPR spacer integration.
Title: Cas1-Cas2 complex formation mediates spacer acquisition during CRISPR-Cas adaptive immunity.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General protein information

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Preferred Names: multifunctional nuclease Cas1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

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Reference assembly

Genomic

NC_000913.3 Reference assembly

Range

2878855..2879772 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NP_417235.1 multifunctional nuclease Cas1 [Escherichia coli str. K-12 substr. MG1655]

See identical proteins and their annotated locations for NP_417235.1

Status: PROVISIONAL

UniProtKB/TrEMBL

A0A7L5V7L1

Conserved Domains (1) summary

TIGR03638
Location:6 → 272

cas1_ECOLI; CRISPR-associated endonuclease Cas1, subtype I-E/ECOLI