lepB signal peptidase I [Escherichia coli str. K-12 substr. MG1655] - Gene

Summary

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Gene symbol: lepB
Gene description: signal peptidase I
Primary source: ASAP:ABE-0008450
Locus tag: b2568
See related: ECOCYC:EG10530
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage: Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as: ECK2566; lep
Summary: LepB has a blocked N-terminus. [More information is available at EcoGene: EG10530]. Signal peptidase catalyzes the cleavage of the amino-terminal leader peptide from secretory proteins. [More information is available at EcoCyc: EG10530].
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Genomic context

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Sequence:: NC_000913.3 (2704335..2705309, complement)

NC_000913.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Expression of the Bacillus subtilis TasA signal peptide leads to cell death in Escherichia coli due to inefficient cleavage by LepB.
Title: Expression of the Bacillus subtilis TasA signal peptide leads to cell death in Escherichia coli due to inefficient cleavage by LepB.
Cotranslational Translocation and Folding of a Periplasmic Protein Domain in Escherichia coli.
Title: Cotranslational Translocation and Folding of a Periplasmic Protein Domain in Escherichia coli.
LepB promote the association of the central domain of colicin D with the inner membrane and translocation of the toxic tRNase domain into the cytoplasm.
Title: The Stable Interaction Between Signal Peptidase LepB of Escherichia coli and Nuclease Bacteriocins Promotes Toxin Entry into the Cytoplasm.
A thorough investigation of different roles of Escherichia coli type I signal peptidase residues binding to lipopeptide inhibitor has been performed by a combination of computational alanine scanning mutagenesis and free energy decomposition methods.
Title: Computational alanine scanning and free energy decomposition for E. coli type I signal peptidase with lipopeptide inhibitor complex.
Ile-144 and Ile-86 contribute to the signal peptidase substrate specificity and Ile-144 is important for the accuracy of the cleavage reaction
Title: The identification of residues that control signal peptidase cleavage fidelity and substrate specificity.
Ile-144 and Ile-86 are key P3 substrate specificity determinants for signal peptidase I
Title: Altered -3 substrate specificity of Escherichia coli signal peptidase 1 mutants as revealed by screening a combinatorial peptide library.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by EcoCyc

Function	Evidence Code	Pubs
enables endopeptidase activity	IDA Inferred from Direct Assay more info	PubMed
enables endopeptidase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables peptidase activity	IDA Inferred from Direct Assay more info	PubMed
enables peptidase activity	IEA Inferred from Electronic Annotation more info
enables peptidase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables serine-type endopeptidase activity	IBA Inferred from Biological aspect of Ancestor more info
enables serine-type endopeptidase activity	IEA Inferred from Electronic Annotation more info
enables serine-type endopeptidase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables serine-type peptidase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within protein processing	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within proteolysis	IDA Inferred from Direct Assay more info	PubMed
involved_in proteolysis	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within proteolysis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in signal peptide processing	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within signal peptide processing	IDA Inferred from Direct Assay more info	PubMed
involved_in signal peptide processing	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within signal peptide processing	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in membrane	IEA Inferred from Electronic Annotation more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: signal peptidase I

NP_417063.1

EC 3.4.21.89

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_000913.3 Reference assembly

Range

2704335..2705309 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NP_417063.1 signal peptidase I [Escherichia coli str. K-12 substr. MG1655]

See identical proteins and their annotated locations for NP_417063.1

Status: PROVISIONAL

UniProtKB/TrEMBL

A0A376WXH9

Conserved Domains (1) summary

PRK10861
Location:1 → 324

PRK10861; signal peptidase I