PFA3 palmitoyltransferase PFA3 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: PFA3
Gene description: palmitoyltransferase PFA3
Primary source: SGD:S000005270
Locus tag: YNL326C
See related: AllianceGenome:SGD:S000005270
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables palmitoyltransferase activity. Involved in protein palmitoylation and vacuole fusion, non-autophagic. Located in fungal-type vacuole membrane. Human ortholog(s) of this gene implicated in non-syndromic X-linked intellectual disability 91. Orthologous to human ZDHHC15 (zinc finger DHHC-type palmitoyltransferase 15); ZDHHC2 (zinc finger DHHC-type palmitoyltransferase 2); and ZDHHC20 (zinc finger DHHC-type palmitoyltransferase 20). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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Location:: chromosome: XIV

Exon count:: 1

Sequence:: Chromosome: XIV; NC_001146.8 (27337..28347, complement)

Chromosome XIV - NC_001146.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001146.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Data show that for Pfa3 and its substrate Vac8, there are two consecutive steps in the acylation reaction: initial binding independent of cysteine in the DHHC box, but requiring myristoylation of Vac8, and a DHHC-motif dependent acylation.
Title: Analysis of DHHC acyltransferases implies overlapping substrate specificity and a two-step reaction mechanism.
Data describe a novel 16-amino-acid motif present at the cytosolic C-terminus of palmitoyltransferases, that is required for Swf1 and Pfa3 function in vivo.
Title: A novel motif at the C-terminus of palmitoyltransferases is essential for Swf1 and Pfa3 function in vivo.
The DHHC protein Pfa3 affects vacuole-associated palmitoylation of the fusion factor Vac8.
Title: The DHHC protein Pfa3 affects vacuole-associated palmitoylation of the fusion factor Vac8.
This study focusses on the function of PFA3 as the third member of the DHHC-CRD family found to have protein acyltransferase activity.
Title: The vacuolar DHHC-CRD protein Pfa3p is a protein acyltransferase for Vac8p.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

D1S3690 (e-PCR)
- UniSTS:474268

Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables acyltransferase activity	IEA Inferred from Electronic Annotation more info
enables palmitoyltransferase activity	IDA Inferred from Direct Assay more info	PubMed
enables palmitoyltransferase activity	IEA Inferred from Electronic Annotation more info
enables palmitoyltransferase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables protein-cysteine S-palmitoyltransferase activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein-cysteine S-palmitoyltransferase activity	IEA Inferred from Electronic Annotation more info
enables zinc ion binding	RCA inferred from Reviewed Computational Analysis more info	PubMed

Process	Evidence Code	Pubs
involved_in peptidyl-L-cysteine S-palmitoylation	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein palmitoylation	IDA Inferred from Direct Assay more info	PubMed
involved_in protein targeting to membrane	IBA Inferred from Biological aspect of Ancestor more info
involved_in vacuole fusion, non-autophagic	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in Golgi apparatus	IBA Inferred from Biological aspect of Ancestor more info
is_active_in endoplasmic reticulum	IBA Inferred from Biological aspect of Ancestor more info
located_in fungal-type vacuole	HDA more info	PubMed
located_in fungal-type vacuole	IDA Inferred from Direct Assay more info	PubMed
located_in fungal-type vacuole	IMP Inferred from Mutant Phenotype more info	PubMed
located_in fungal-type vacuole membrane	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in vacuolar membrane	IEA Inferred from Electronic Annotation more info
located_in vacuole	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: palmitoyltransferase PFA3

NP_014073.1

EC 2.3.1.225

Palmitoyltransferase for Vac8p; required for vacuolar membrane fusion; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; autoacylates; required for vacuolar integrity under stress conditions

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001146.8 Reference assembly

Range

27337..28347 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001183164.1 → NP_014073.1 TPA: palmitoyltransferase PFA3 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_014073.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W0M1, P42836

UniProtKB/TrEMBL

A0A8H4BVD6, G2WLE5, N1NZX4

Conserved Domains (1) summary

COG5273
Location:1 → 304

COG5273; Uncharacterized protein containing DHHC-type Zn finger [General function prediction only]