SGS1 ATP-dependent DNA helicase SGS1 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: SGS1
Gene description: ATP-dependent DNA helicase SGS1
Primary source: SGD:S000004802
Locus tag: YMR190C
See related: AllianceGenome:SGD:S000004802
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables DNA helicase activity. Involved in several processes, including DNA metabolic process; nuclear division; and regulation of cell cycle process. Located in nucleolus. Part of RecQ family helicase-topoisomerase III complex. Used to study Bloom syndrome and Werner syndrome. Human ortholog(s) of this gene implicated in Bloom syndrome; hepatocellular carcinoma; lung cancer; and pancreatic cancer. Orthologous to several human genes including RECQL (RecQ like helicase). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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Location:: chromosome: XIII

Exon count:: 1

Sequence:: Chromosome: XIII; NC_001145.3 (640915..645258, complement)

Chromosome XIII - NC_001145.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001145.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Checkpoint phosphorylation sites on budding yeast Rif1 protect nascent DNA from degradation by Sgs1-Dna2.
Title: Checkpoint phosphorylation sites on budding yeast Rif1 protect nascent DNA from degradation by Sgs1-Dna2.
Multifaceted regulation of the sumoylation of the Sgs1 DNA helicase.
Title: Multifaceted regulation of the sumoylation of the Sgs1 DNA helicase.
C-Terminal HA Tags Compromise Function and Exacerbate Phenotypes of Saccharomyces cerevisiae Bloom's Helicase Homolog Sgs1 SUMOylation-Associated Mutants.
Title: C-Terminal HA Tags Compromise Function and Exacerbate Phenotypes of Saccharomyces cerevisiae Bloom's Helicase Homolog Sgs1 SUMOylation-Associated Mutants.
Comprehensive Synthetic Genetic Array Analysis of Alleles That Interact with Mutation of the Saccharomyces cerevisiae RecQ Helicases Hrq1 and Sgs1.
Title: Comprehensive Synthetic Genetic Array Analysis of Alleles That Interact with Mutation of the Saccharomyces cerevisiae RecQ Helicases Hrq1 and Sgs1.
Phosphorylation of the RecQ Helicase Sgs1/BLM Controls Its DNA Unwinding Activity during Meiosis and Mitosis.
Title: Phosphorylation of the RecQ Helicase Sgs1/BLM Controls Its DNA Unwinding Activity during Meiosis and Mitosis.
Maintenance of Yeast Genome Integrity by RecQ Family DNA Helicases.
Title: Maintenance of Yeast Genome Integrity by RecQ Family DNA Helicases.
Deletion of ULS1 confers damage tolerance in sgs1 mutants through a Top3-dependent D-loop mediated fork restart pathway
Title: Deletion of ULS1 confers damage tolerance in sgs1 mutants through a Top3-dependent D-loop mediated fork restart pathway.
Results suggest that the differential regulation of Sgs1 activity by its protein partners is important to support diverse cellular functions of Sgs1 during the maintenance of genome stability.
Title: Competing interaction partners modulate the activity of Sgs1 helicase during DNA end resection.
we find that Sgs1 utilizes a novel motor mechanism for disrupting ssDNA intermediates bound by the recombinase protein Rad51. The ability of Sgs1 to disrupt Rad51-ssDNA filaments may explain some of the defects engendered by RECQ helicase deficiencies in human cells
Title: The RecQ helicase Sgs1 drives ATP-dependent disruption of Rad51 filaments.
DNA nick created by MRX-Sae2 at Ku-blocked DNA double-strand breaks ends can serve as the entry site for Exo1 or Sgs1-Dna2.
Title: A DNA nick at Ku-blocked double-strand break ends serves as an entry site for exonuclease 1 (Exo1) or Sgs1-Dna2 in long-range DNA end resection.

Submit: New GeneRIF Correction See all GeneRIFs (67)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables 3'-5' DNA helicase activity	IBA Inferred from Biological aspect of Ancestor more info
enables 3'-5' DNA helicase activity	IDA Inferred from Direct Assay more info	PubMed
enables 3'-5' DNA helicase activity	IEA Inferred from Electronic Annotation more info
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP hydrolysis activity	IEA Inferred from Electronic Annotation more info
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA helicase activity	IDA Inferred from Direct Assay more info	PubMed
enables four-way junction helicase activity	IBA Inferred from Biological aspect of Ancestor more info
enables helicase activity	IEA Inferred from Electronic Annotation more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables isomerase activity	IEA Inferred from Electronic Annotation more info
enables nucleic acid binding	IEA Inferred from Electronic Annotation more info
enables zinc ion binding	RCA inferred from Reviewed Computational Analysis more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA double-strand break processing	IGI Inferred from Genetic Interaction more info	PubMed
involved_in DNA double-strand break processing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in DNA duplex unwinding	IDA Inferred from Direct Assay more info	PubMed
involved_in DNA duplex unwinding	IEA Inferred from Electronic Annotation more info
involved_in DNA recombination	IEA Inferred from Electronic Annotation more info
involved_in DNA repair	IEA Inferred from Electronic Annotation more info
involved_in DNA replication	IEA Inferred from Electronic Annotation more info
involved_in DNA topological change	IDA Inferred from Direct Assay more info	PubMed
involved_in DNA unwinding involved in DNA replication	IBA Inferred from Biological aspect of Ancestor more info
involved_in DNA unwinding involved in DNA replication	IDA Inferred from Direct Assay more info	PubMed
involved_in G-quadruplex DNA unwinding	IDA Inferred from Direct Assay more info	PubMed
involved_in chromosome organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in double-strand break repair via homologous recombination	IBA Inferred from Biological aspect of Ancestor more info
involved_in double-strand break repair via homologous recombination	IGI Inferred from Genetic Interaction more info	PubMed
involved_in double-strand break repair via homologous recombination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in gene conversion at mating-type locus	IGI Inferred from Genetic Interaction more info	PubMed
involved_in meiotic DNA double-strand break processing	IGI Inferred from Genetic Interaction more info	PubMed
involved_in meiotic chromosome segregation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic G2/M transition checkpoint	IGI Inferred from Genetic Interaction more info	PubMed
involved_in mitotic intra-S DNA damage checkpoint signaling	IGI Inferred from Genetic Interaction more info	PubMed
involved_in mitotic intra-S DNA damage checkpoint signaling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic sister chromatid segregation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of meiotic joint molecule formation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in nucleic acid metabolic process	IEA Inferred from Electronic Annotation more info
involved_in regulation of reciprocal meiotic recombination	IGI Inferred from Genetic Interaction more info	PubMed
involved_in telomere maintenance	IGI Inferred from Genetic Interaction more info	PubMed
involved_in telomere maintenance via recombination	IGI Inferred from Genetic Interaction more info	PubMed
involved_in telomere maintenance via recombination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in telomeric 3' overhang formation	IGI Inferred from Genetic Interaction more info	PubMed

Component	Evidence Code	Pubs
part_of RecQ family helicase-topoisomerase III complex	IDA Inferred from Direct Assay more info	PubMed
part_of RecQ family helicase-topoisomerase III complex	IPI Inferred from Physical Interaction more info	PubMed
is_active_in chromosome	IBA Inferred from Biological aspect of Ancestor more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in intracellular organelle	IEA Inferred from Electronic Annotation more info
is_active_in nucleolus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleolus	IEA Inferred from Electronic Annotation more info
located_in nucleus	HDA more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IEA Inferred from Electronic Annotation more info
part_of protein-containing complex	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: ATP-dependent DNA helicase SGS1

NP_013915.1

EC 3.6.4.12

RecQ family nucleolar DNA helicase; role in genome integrity maintenance, chromosome synapsis, meiotic joint molecule/crossover formation; stimulates activity of Top3p; rapidly lost in response to rapamycin in Rrd1p-dependent manner; forms nuclear foci upon DNA replication stress; yeast SGS1 complements mutations in human homolog BLM implicated in Bloom syndrome; also similar to human WRN implicated in Werner syndrome; human BLM and WRN can each complement yeast null mutant

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001145.3 Reference assembly

Range

640915..645258 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001182696.1 → NP_013915.1 TPA: ATP-dependent DNA helicase SGS1 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_013915.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W014, P35187

UniProtKB/TrEMBL

A0A8H4F849, C7GRG1

Conserved Domains (1) summary

COG0514
Location:670 → 1350

RecQ; Superfamily II DNA helicase RecQ [Replication, recombination and repair]