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UBC7 E2 ubiquitin-conjugating protein UBC7 [ Saccharomyces cerevisiae S288C ]

Gene ID: 855036, updated on 11-Apr-2024

Summary

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Gene symbol
UBC7
Gene description
E2 ubiquitin-conjugating protein UBC7
Primary source
SGD:S000004624
Locus tag
YMR022W
See related
AllianceGenome:SGD:S000004624
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as
DER2; QRI8
Summary
Enables ubiquitin-protein transferase activity. Involved in chromatin assembly or disassembly; fungal-type cell wall organization; and ubiquitin-dependent ERAD pathway. Located in endoplasmic reticulum membrane. Part of Doa10p ubiquitin ligase complex and Hrd1p ubiquitin ligase ERAD-L complex. Orthologous to human UBE2G2 (ubiquitin conjugating enzyme E2 G2). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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Location:
chromosome: XIII
Exon count:
1
Sequence:
Chromosome: XIII; NC_001145.3 (318680..319177)

Chromosome XIII - NC_001145.3Genomic Context describing neighboring genes Neighboring gene polyamine oxidase Neighboring gene Mac1p Neighboring gene Mss1p Neighboring gene tRNA-Ala

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_001145.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Assays for dissecting the in vitro enzymatic activity of yeast Ubc7. Mashahreh B, et al. Methods Enzymol, 2019. PMID 30910030
  2. Ubiquitin-mediated regulation of 3-hydroxy-3-methylglutaryl-CoA reductase. Hampton RY, et al. Proc Natl Acad Sci U S A, 1997 Nov 25. PMID 9371780, Free PMC Article
  3. Resistance to cadmium mediated by ubiquitin-dependent proteolysis. Jungmann J, et al. Nature, 1993 Jan 28. PMID 8381213
  4. QRI8, a novel ubiquitin-conjugating enzyme in Saccharomyces cerevisiae. Vassal A, et al. Biochim Biophys Acta, 1992 Sep 24. PMID 1327148
  5. Hrd1 forms the retrotranslocation pore regulated by auto-ubiquitination and binding of misfolded proteins. Vasic V, et al. Nat Cell Biol, 2020 Mar. PMID 32094691

See all (140) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Interaction of Ubc7 with the RING domains of its cognate E3 Ub-protein ligases stimulates the formation of isopeptide (amide) Ub-Ub linkages. This article provides protocols for dissecting the in vitro enzymatic activity of yeast Ubc7.
    Title: Assays for dissecting the in vitro enzymatic activity of yeast Ubc7.
  2. Sequential poly-ubiquitylation by specialized conjugating enzymes Ubc6 and Ubc7 expands the versatility of a quality control ubiquitin ligase Doa10.
    Title: Sequential Poly-ubiquitylation by Specialized Conjugating Enzymes Expands the Versatility of a Quality Control Ubiquitin Ligase.
  3. Cue1 positions Ubc7 and the distal acceptor ubiquitin for rapid polyubiquitination.
    Title: The CUE Domain of Cue1 Aligns Growing Ubiquitin Chains with Ubc7 for Rapid Elongation.
  4. Data indicate the crystal structure of the Ubc7p-binding region (U7BR) of Cue1p with Ubc7p.
    Title: A structurally unique E2-binding domain activates ubiquitination by the ERAD E2, Ubc7p, through multiple mechanisms.
  5. Data show that a discrete C-terminal Ubc7p binding region of Cue1p is required for ER-associated degradation and for Ubc7p-dependent ubiquitylation by Hrd1p in vitro.
    Title: A Ubc7p-binding domain in Cue1p activates ER-associated protein degradation.
  6. reveal a previously unobserved stimulation of Ubc7p E2 activity by Cue1p
    Title: Cue1p is an activator of Ubc7p E2 activity in vitro and in vivo.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables transferase activity IEA
Inferred from Electronic Annotation
more info
  
enables ubiquitin conjugating enzyme activity IEA
Inferred from Electronic Annotation
more info
  
enables ubiquitin-protein transferase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables ubiquitin-protein transferase activity ISS
Inferred from Sequence or Structural Similarity
more info
 PubMed 
Process Evidence Code Pubs
involved_in ERAD pathway IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in ERAD pathway IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in ERAD pathway IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in ERAD pathway IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in chromatin organization IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in fungal-type cell wall organization IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in protein ubiquitination IEA
Inferred from Electronic Annotation
more info
  
involved_in response to cadmium ion IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
part_of Doa10p ubiquitin ligase complex IDA
Inferred from Direct Assay
more info
 PubMed 
part_of Hrd1p ubiquitin ligase ERAD-L complex IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in endoplasmic reticulum HDA PubMed 
is_active_in endoplasmic reticulum IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in endoplasmic reticulum IEA
Inferred from Electronic Annotation
more info
  
located_in endoplasmic reticulum membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in endoplasmic reticulum membrane IEA
Inferred from Electronic Annotation
more info
  
located_in membrane IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
E2 ubiquitin-conjugating protein UBC7
NP_013735.1
  • Ubiquitin conjugating enzyme; involved in the ER-associated protein degradation (ERAD) pathway and in the inner nuclear membrane-associated degradation (INMAD) pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001145.3 Reference assembly

    Range
    318680..319177
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001182518.1 → NP_013735.1  TPA: E2 ubiquitin-conjugating protein UBC7 [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_013735.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6VZJ6, Q02159
    UniProtKB/TrEMBL
    A0A6A5PRC4, A6ZM84, B3LLS3, C7GL97, C8ZEM9, G2WK73, N1P061
    Conserved Domains (1) summary
    COG5078
    Location:1 → 165
    COG5078; Ubiquitin-protein ligase [Posttranslational modification, protein turnover, chaperones]
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q02159.1

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