DAM1 Dam1p [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: DAM1
Gene description: Dam1p
Primary source: SGD:S000003345
Locus tag: YGR113W
See related: AllianceGenome:SGD:S000003345
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Contributes to microtubule plus-end binding activity. Involved in attachment of spindle microtubules to kinetochore; positive regulation of attachment of spindle microtubules to kinetochore; and positive regulation of microtubule polymerization. Part of DASH complex. [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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Location:: chromosome: VII

Exon count:: 1

Sequence:: Chromosome: VII; NC_001139.9 (718893..719924)

Chromosome VII - NC_001139.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001139.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Structure of the Ndc80 complex and its interactions at the yeast kinetochore-microtubule interface.
Title: Structure of the Ndc80 complex and its interactions at the yeast kinetochore-microtubule interface.
Three interacting regions of the Ndc80 and Dam1 complexes support microtubule tip-coupling under load.
Title: Three interacting regions of the Ndc80 and Dam1 complexes support microtubule tip-coupling under load.
Cdk1 Phosphorylation of the Dam1 Complex Strengthens Kinetochore-Microtubule Attachments.
Title: Cdk1 Phosphorylation of the Dam1 Complex Strengthens Kinetochore-Microtubule Attachments.
Our results suggest the stoichiometry of outer kinetochore submodules is strongly influenced by factors at the kinetochore-microtubule interface such as Fin1 and Dam1, and phosphorylation by cyclin-dependent kinase.
Title: The stoichiometry of the outer kinetochore is modulated by microtubule-proximal regulatory factors.
Dam1 promotes centromere clustering through stabilization of the kinetochore integrity.
Title: Outer kinetochore protein Dam1 promotes centromere clustering in parallel with Slk19 in budding yeast.
In metaphase, approximately 40% of the Dam1C/DASH assemblies are complete rings; the rest are partial rings. Ring completeness and binding position along the microtubule are sensitive to kinetochore attachment and tension, respectively.
Title: Electron cryotomography analysis of Dam1C/DASH at the kinetochore-spindle interface in situ.
regulation of Dam1 phosphorylation imposed by Ipl1 kinase is critical for faithful chromosome segregation.
Title: The phosphorylation of a kinetochore protein Dam1 by Aurora B/Ipl1 kinase promotes chromosome bipolar attachment in yeast.
PKA site of Dam1p participates in regulating kinetochore activity.
Title: Glucose Signaling Is Connected to Chromosome Segregation Through Protein Kinase A Phosphorylation of the Dam1 Kinetochore Subunit in Saccharomycescerevisiae.
One meiotic role of Mps1 is to stabilize connections that have been established between kinetochores and microtubles by phosphorylating Dam1.
Title: Mps1 promotes chromosome meiotic chromosome biorientation through Dam1.
this study reports the cryo-electron microscopy structure (at ~4.5-angstrom resolution) of a DASH/Dam1c ring and a molecular model of its ordered components, validated by evolutionary direct-coupling analysis.
Title: Structure of the DASH/Dam1 complex shows its role at the yeast kinetochore-microtubule interface.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables microtubule binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in attachment of spindle microtubules to kinetochore	IDA Inferred from Direct Assay more info	PubMed
involved_in attachment of spindle microtubules to kinetochore	IEA Inferred from Electronic Annotation more info
involved_in cell cycle	IEA Inferred from Electronic Annotation more info
involved_in cell division	IEA Inferred from Electronic Annotation more info
involved_in centromere clustering	IDA Inferred from Direct Assay more info	PubMed
involved_in chromosome segregation	IEA Inferred from Electronic Annotation more info
involved_in mitotic sister chromatid biorientation	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of attachment of spindle microtubules to kinetochore	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of microtubule polymerization	IDA Inferred from Direct Assay more info	PubMed
involved_in protein localization to chromosome, centromeric region	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
part_of DASH complex	IBA Inferred from Biological aspect of Ancestor more info
part_of DASH complex	IDA Inferred from Direct Assay more info	PubMed
part_of DASH complex	IEA Inferred from Electronic Annotation more info
located_in chromosome	IEA Inferred from Electronic Annotation more info
located_in chromosome, centromeric region	IEA Inferred from Electronic Annotation more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoskeleton	IEA Inferred from Electronic Annotation more info
located_in kinetochore	IEA Inferred from Electronic Annotation more info
located_in microtubule	IEA Inferred from Electronic Annotation more info
located_in mitotic spindle	IEA Inferred from Electronic Annotation more info
is_active_in mitotic spindle polar microtubule	IBA Inferred from Biological aspect of Ancestor more info
is_active_in mitotic spindle pole body	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in spindle	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: Dam1p

NP_011628.4

Essential subunit of the Dam1 complex (aka DASH complex); cooperates with Duo1p and Pse1p to connect the DASH complex with the microtubules (MT); couples kinetochores to the force produced by MT depolymerization, thereby aiding in chromosome segregation; N-terminal staple region is important for self-assembly of the Dam1 complex higher order ring structure that forms around microtubules; Ipl1p target for regulating kinetochore-MT attachments