PRP19 E3 ubiquitin-protein ligase PRP19 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: PRP19
Gene description: E3 ubiquitin-protein ligase PRP19
Primary source: SGD:S000003959
Locus tag: YLL036C
See related: AllianceGenome:SGD:S000003959
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: PSO4
Summary: Enables ubiquitin-protein transferase activity. Involved in generation of catalytic spliceosome for first transesterification step. Located in cytosol and nucleus. Part of Prp19 complex and U2-type catalytic step 1 spliceosome. Orthologous to human PRPF19 (pre-mRNA processing factor 19). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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Location:: chromosome: XII

Exon count:: 1

Sequence:: Chromosome: XII; NC_001144.5 (66745..68256, complement)

Chromosome XII - NC_001144.5 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001144.5

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

A distinct complex of PRP19-related and trypanosomatid-specific proteins is required for pre-mRNA splicing in trypanosomes.
Title: A distinct complex of PRP19-related and trypanosomatid-specific proteins is required for pre-mRNA splicing in trypanosomes.
we provide the first piece of evidence that the linker sequences outside the RNA recognition motif (RRM) - those connecting the ZnF domain and the RRM of the Prp19-associated complex - directly interact with RNA
Title: Structure of the mRNA splicing complex component Cwc2: insights into RNA recognition.
New low abundance splicing factors connected to NTC (Nineteen Complex) function, provides evidence for distinct Prp19 containing complexes, and underscores the role of the Prp19 WD40 domain as a splicing scaffold.
Title: Systematic two-hybrid and comparative proteomic analyses reveal novel yeast pre-mRNA splicing factors connected to Prp19.
hPRP19 interacts with PHD3 to suppress the cell death under hypoxic conditions by limiting the function of PHD3 which leads to caspase activation
Title: Human PRP19 interacts with prolyl-hydroxylase PHD3 and inhibits cell death in hypoxia.
The Prp19-associated complex has an indirect role in spliceosome recycling through affecting the biogenesis of U4/U6 snRNP in the in vivo splicing reaction.
Title: Functional links between the Prp19-associated complex, U4/U6 biogenesis, and spliceosome recycling.
Prp19p forms a tetramer in vitro and in vivo; the domain required for its oligomerization maps to a central tetrameric coiled-coil
Title: Structural and functional analysis of essential pre-mRNA splicing factor Prp19p.
Yju2 acts in concert with an unidentified heat-resistant factor(s) in an ATP-independent manner to promote the first catalytic reaction of pre-mRNA splicing after Prp2-mediated structural rearrangement of the spliceosome.
Title: A novel splicing factor, Yju2, is associated with NTC and acts after Prp2 in promoting the first catalytic reaction of pre-mRNA splicing.
Data suggest that the Prp19-associated complex is required for defining the specificity of interaction of U5 and U6 with the 5' splice site to stabilize their association with the spliceosome after U4 is dissociated.
Title: The Prp19-associated complex is required for specifying interactions of U5 and U6 with pre-mRNA during spliceosome activation.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables transferase activity	IEA Inferred from Electronic Annotation more info
enables ubiquitin protein ligase activity	IEA Inferred from Electronic Annotation more info
enables ubiquitin-protein transferase activity	IBA Inferred from Biological aspect of Ancestor more info
enables ubiquitin-protein transferase activity	IDA Inferred from Direct Assay more info	PubMed
enables ubiquitin-protein transferase activity	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA repair	IEA Inferred from Electronic Annotation more info
involved_in RNA splicing	IEA Inferred from Electronic Annotation more info
involved_in generation of catalytic spliceosome for first transesterification step	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mRNA processing	IEA Inferred from Electronic Annotation more info
involved_in mRNA splicing, via spliceosome	IBA Inferred from Biological aspect of Ancestor more info
involved_in mRNA splicing, via spliceosome	IEA Inferred from Electronic Annotation more info
involved_in protein ubiquitination	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
part_of Prp19 complex	IBA Inferred from Biological aspect of Ancestor more info
part_of Prp19 complex	IDA Inferred from Direct Assay more info	PubMed
part_of Prp19 complex	IEA Inferred from Electronic Annotation more info
part_of U2-type catalytic step 1 spliceosome	IBA Inferred from Biological aspect of Ancestor more info
part_of U2-type catalytic step 1 spliceosome	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	HDA more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrion	HDA more info	PubMed
located_in nucleus	HDA more info	PubMed
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info
part_of spliceosomal complex	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: E3 ubiquitin-protein ligase PRP19

NP_013064.1

EC 2.3.2.27

Splicing factor associated with the spliceosome; contains a U-box, a motif found in a class of ubiquitin ligases, and a WD40 domain; relocalizes to the cytosol in response to hypoxia

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001144.5 Reference assembly

Range

66745..68256 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001181856.1 → NP_013064.1 TPA: E3 ubiquitin-protein ligase PRP19 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_013064.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VXW9, P32523, Q07870

UniProtKB/TrEMBL

A0A8H4BXT2, E9P8H9, N1NZD9

Conserved Domains (3) summary

COG2319
Location:264 → 503

WD40; WD40 repeat [General function prediction only]

cd16656
Location:2 → 53

RING-Ubox_PRP19; U-box domain, a modified RING finger, found in pre-mRNA-processing factor 19 (Prp19) and similar proteins

pfam08606
Location:75 → 132

Prp19; Prp19/Pso4-like

Nucleotide		Protein
Heading	Accession and Version	Protein

Protein Accession	Links
Protein Accession	GenPept Link	UniProtKB Link
5GM6_o.1
5GM6_p.1
5GM6_q.1
5GM6_r.1
5GMK_o.1
5GMK_p.1
5GMK_q.1
5GMK_r.1
5WSG_o.1
5WSG_p.1
5WSG_q.1
5WSG_r.1
5Y88_q.1
5Y88_r.1
5Y88_s.1
5Y88_t.1
5YLZ_q.1
5YLZ_r.1
5YLZ_s.1
5YLZ_t.1
6BK8_u.1
6BK8_v.1
6BK8_w.1
6BK8_x.1
6EXN_t.1
6EXN_u.1
6EXN_v.1
6EXN_w.1
6J6G_o.1
6J6G_p.1
6J6G_q.1
6J6G_r.1
6J6H_o.1
6J6H_p.1
6J6H_q.1
6J6H_r.1
6J6N_o.1
6J6N_p.1
6J6N_q.1
6J6N_r.1
6J6Q_o.1
6J6Q_p.1
6J6Q_q.1
6J6Q_r.1
P32523.2