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PRORP2 proteinaceous RNase P 2 [ Arabidopsis thaliana (thale cress) ]

Gene ID: 816166, updated on 7-Sep-2023

Summary

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Gene symbol
PRORP2
Gene description
proteinaceous RNase P 2
Primary source
Araport:AT2G16650
Locus tag
AT2G16650
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Arabidopsis thaliana (ecotype: Columbia)
Lineage
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as
proteinaceous RNase P 2; T24I21.6; T24I21_6
Summary
Encodes a proteinaceous RNase P that supports RNase P activity in vivo in both organelles and the nucleus. It is also involved in the maturation of small nucleolar RNA (snoRNA) and mRNA.
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Genomic context

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Location:
chromosome: 2
Exon count:
7
Sequence:
Chromosome: 2; NC_003071.7 (7215591..7218558, complement)

Chromosome 2 - NC_003071.7Genomic Context describing neighboring genes Neighboring gene Protein kinase superfamily protein Neighboring gene Pollen Ole e 1 allergen and extensin family protein Neighboring gene multimeric translocon complex in the outer envelope membrane 132 Neighboring gene Major facilitator superfamily protein Neighboring gene pseudo

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_003071.7

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Nuclear Protein-Only Ribonuclease P2 Structure and Biochemical Characterization Provide Insight into the Conserved Properties of tRNA 5' End Processing Enzymes. Karasik A, et al. J Mol Biol, 2016 Jan 16. PMID 26655022, Free PMC Article
  2. Crystallization and crystallographic analysis of an Arabidopsis nuclear proteinaceous RNase P. Pinker F, et al. Acta Crystallogr F Struct Biol Commun, 2015 Nov. PMID 26527263, Free PMC Article
  3. Biophysical analysis of Arabidopsis protein-only RNase P alone and in complex with tRNA provides a refined model of tRNA binding. Pinker F, et al. J Biol Chem, 2017 Aug 25. PMID 28696260, Free PMC Article
  4. tRNA processing by protein-only versus RNA-based RNase P: kinetic analysis reveals mechanistic differences. Pavlova LV, et al. Chembiochem, 2012 Oct 15. PMID 22976545
  5. A single Arabidopsis organellar protein has RNase P activity. Gobert A, et al. Nat Struct Mol Biol, 2010 Jun. PMID 20473316

See all (8) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. The comparative biology of Escherichia coli RNase P and Arabidopsis thaliana PRORP isozymes has been reported.
    Title: Protein-only RNase P function in Escherichia coli: viability, processing defects and differences between PRORP isoenzymes.
  2. Data suggest that PRORP2 undergoes conformational changes to accommodate its substrate (precursor tRNA) and product (mature tRNA); the catalytic domain and the RNA-binding domain appear to move around a central hinge; cysteine-specific tRNA(GCA) was used in these experiments.
    Title: Biophysical analysis of Arabidopsis protein-only RNase P alone and in complex with tRNA provides a refined model of tRNA binding.
  3. The purification and crystallization of PRORP2 from Arabidopsis was identified.
    Title: Crystallization and crystallographic analysis of an Arabidopsis nuclear proteinaceous RNase P.
  4. The authors solved the crystal structure of PRORP2 (3.2A) revealing an overall V-shaped protein and conserved metallonuclease active-site structure.
    Title: Nuclear Protein-Only Ribonuclease P2 Structure and Biochemical Characterization Provide Insight into the Conserved Properties of tRNA 5' End Processing Enzymes.
  5. The study presents the first kinetic analysis of PRORP1, PRORP2 and PRORP3.
    Title: tRNA processing by protein-only versus RNA-based RNase P: kinetic analysis reveals mechanistic differences.
Submit: New GeneRIF Correction

General gene information

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Homology

NM_001335484.1
NM_127217.4

Gene Ontology Provided by TAIR

Function Evidence Code Pubs
enables ribonuclease P activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables ribonuclease P activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
acts_upstream_of_or_within mRNA processing IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within sno(s)RNA processing IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within tRNA 5'-leader removal IDA
Inferred from Direct Assay
more info
 PubMed 
Component Evidence Code Pubs
located_in chloroplast ISM
Inferred from Sequence Model
more info
  
located_in nucleus IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nucleus ISM
Inferred from Sequence Model
more info
  

General protein information

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Preferred Names
proteinaceous RNase P 2
NP_179256.3
  • proteinaceous RNase P 2 (PRORP2); FUNCTIONS IN: antiporter activity, drug transmembrane transporter activity; INVOLVED IN: drug transmembrane transport, transmembrane transport; LOCATED IN: nucleus; EXPRESSED IN: male gametophyte, cultured cell; EXPRESSED DURING: M germinated pollen stage; CONTAINS InterPro DOMAIN/s: Ribonuclease Zc3h12a-like (InterPro:IPR021869), Multi antimicrobial extrusion protein MatE (InterPro:IPR002528); BEST Arabidopsis thaliana protein match is: proteinaceous RNase P 3 (TAIR:AT4G21900.1); Has 397 Blast hits to 377 proteins in 89 species: Archae - 0; Bacteria - 0; Metazoa - 82; Fungi - 0; Plants - 249; Viruses - 0; Other Eukaryotes - 66 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_003071.7 Reference assembly

    Range
    7215591..7218558 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_127217.4 → NP_179256.3  proteinaceous RNase P 2 [Arabidopsis thaliana]

    See identical proteins and their annotated locations for NP_179256.3

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    Q680B9, Q9SLF2
    UniProtKB/TrEMBL
    A0A5S9WYL0, A0A654ETE4, A0A7G2ECQ7
    Conserved Domains (3) summary
    sd00004
    Location:109 → 138
    PPR; PPR repeat [structural motif]
    cd18671
    Location:340 → 466
    PIN_PRORP-Zc3h12a-like; PIN domain of protein-only RNase P (PRORP), ribonuclease Zc3h12a, and related proteins
    pfam17177
    Location:20 → 233
    PPR_long; Pentacotripeptide-repeat region of PRORP

  2. NM_001335484.1 → NP_001324533.1  proteinaceous RNase P 2 [Arabidopsis thaliana]

    Status: REVIEWED

    UniProtKB/TrEMBL
    A0A178VQ12, A0A1P8B0H9, A0A7G2ECQ7
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q680B9.1

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