chk1 protein kinase Chk1 [Schizosaccharomyces pombe (fission yeast)] - Gene

Summary

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Gene symbol: chk1
Gene description: protein kinase Chk1
Locus tag: SPCC1259.13
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Schizosaccharomyces pombe (strain: 972h-)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina; Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces
Also known as: rad27
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Genomic context

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Location:: chromosome: III

Exon count:: 8

Sequence:: Chromosome: III; NC_003421.2 (1059799..1064443)

Chromosome III - NC_003421.2 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003421.2

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

chk1- and wat1-null mutants exhibit conditional synthetic lethality.
Title: Activation of Checkpoint Kinase Chk1 by Reactive Oxygen Species Resulting from Disruption of wat1/pop3 in Schizosaccharomyces pombe.
The isolation of a mutant allele of rna14 in Schizosaccharomyces pombe has been reported that exhibits reduction in protein level of Chk1 at the nonpermissive temperature, primarily due to the defects in posttranscriptional processing.
Title: Mutant allele of rna14 in fission yeast affects pre-mRNA splicing.
Rad4 mainly functions as a scaffold protein in the Chk1, not the Cds1(CHK2 in humans), checkpoint pathway. It plays a minor role or is functionally redundant with an unknown factor in Rad3 activation.
Title: Rad4 mainly functions in Chk1-mediated DNA damage checkpoint pathway as a scaffold protein in the fission yeast Schizosaccharomyces pombe.
The combination of wat1-17 with the chk1 deletion aggravates the defects in the maintenance of fungal genome ploidy.
Title: Wat1/pop3, a conserved WD repeat containing protein acts synergistically with checkpoint kinase Chk1 to maintain genome ploidy in fission yeast S. pombe.
study describes an analysis of a kinase-associated 1 domain in the C-terminus of Chk1, plus an additional highly conserved motif present in an unique C-terminal extension, both of which are required for Chk1 activation; presence of these motifs provides a rationale as to the dual nature of the regulatory domain
Title: Regulatory motifs in Chk1.
These results suggest that the main role of Crb2 in DNA damage checkpoint signaling is recruiting Chk1 to sites of DNA lesions.
Title: Phosphorylation-dependent interactions between Crb2 and Chk1 are essential for DNA damage checkpoint.
Rather than being an autoinhibitory domain, the C-terminus of Chk1 also contains domains critical for adopting an active configuration.
Title: Regulation of Chk1 by its C-terminal domain.
C-terminal region of Chk1 is important for checkpoint function and predict that a limiting factor capable of associating with Chk1D469G, but not Chk1D469A, interacts with Chk1 to elicit checkpoint activation in response to a subset of DNA lesions
Title: Importance of a C-terminal conserved region of Chk1 for checkpoint function.
Chk1 DNA damage pathway induces sustained activation of the spindle checkpoint
Title: The fission yeast Crb2/Chk1 pathway coordinates the DNA damage and spindle checkpoint in response to replication stress induced by topoisomerase I inhibitor.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by PomBase

Function	Evidence Code	Pubs
enables ATP binding	ISM Inferred from Sequence Model more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein serine/threonine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein serine/threonine kinase activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
NOT involved_in DNA repair	EXP Inferred from Experiment more info	PubMed
involved_in meiotic recombination checkpoint signaling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic DNA damage checkpoint signaling	IGI Inferred from Genetic Interaction more info	PubMed
involved_in mitotic DNA damage checkpoint signaling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic G2 DNA damage checkpoint signaling	EXP Inferred from Experiment more info	PubMed
involved_in mitotic G2 DNA damage checkpoint signaling	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitotic G2 DNA damage checkpoint signaling	IDA Inferred from Direct Assay more info	PubMed
involved_in mitotic G2 DNA damage checkpoint signaling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of G2/M transition of mitotic cell cycle	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein phosphorylation	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
part_of chromatin	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasm	HDA more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
is_active_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytosol	HDA more info	PubMed
is_active_in nucleus	HDA more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
is_active_in nucleus	IDA Inferred from Direct Assay more info	PubMed
is_active_in site of double-strand break	IBA Inferred from Biological aspect of Ancestor more info
is_active_in site of double-strand break	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: protein kinase Chk1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003421.2 Reference assembly

Range

1059799..1064443

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001355907.1 → NP_001342786.1 protein kinase Chk1 [Schizosaccharomyces pombe]

Status: PROVISIONAL

UniProtKB/Swiss-Prot

P34208

Conserved Domains (1) summary

cd14069
Location:8 → 272

STKc_Chk1; Catalytic domain of the Serine/Threonine kinase, Checkpoint kinase 1